Results 111 to 120 of about 20,947 (214)

Impact of rapidly cleaving embryos on blastocyst formation and single-blastocyst transfer outcomes [PDF]

BackgroundClarifying the impact of day-3 cell number on blastulation and subsequent pregnancy outcomes is essential for optimizing blastocyst selection criteria and refining embryo assessment protocols.
Song Quan, Xiao-cong Wang, Wang, Xiao-cong, Wen-jie Huo, Quan, Song, Fei Peng, Chen Luo, Huo, Wen-jie, Peng, Fei, Luo, Chen   +9 more
core   +1 more source

New biosensors and transgenic mice for multiplex cGMP imaging

British Journal of Pharmacology, EarlyView.
Background and Purpose Cyclic guanosine monophosphate (cGMP) is a versatile second messenger that is important for human (patho‐)physiology and pharmacotherapy. Live‐cell imaging of cGMP with biosensors allows to elucidate its spatiotemporal dynamics in real time under close‐to‐native conditions. However, to monitor two separate cGMP pools or cGMP/cAMP
Markus Wolters, Martin Thunemann, Barbara Birk, Susanne Feil, Viacheslav O. Nikolaev, Moritz Lehners, Robert Feil   +6 more
wiley   +1 more source

G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst

eLife, 2018
Early mouse development is regulated and accompanied by dynamic changes in chromatin modifications, including G9a-mediated histone H3 lysine 9 dimethylation (H3K9me2).
Jan J Zylicz, Maud Borensztein, Frederick CK Wong, Yun Huang, Caroline Lee, Sabine Dietmann, M Azim Surani   +6 more
doaj   +1 more source

Overall Blastocyst Quality, Trophectoderm Grade, and Inner Cell Mass Grade Predict Pregnancy Outcome in Euploid Blastocyst Transfer Cycles

, 2018
Background: Despite recent advances that have improved the pregnancy success rates that can be achieved via in vitro fertilization (IVF) therapy, it is not yet clear which blastocyst morphological parameters best predict the outcomes of single blastocyst
Yang Yu, Yan-Yu Zhao, Xiao-Wei Zhang
core   +1 more source

Evidence That the Protein Phosphatase Activity of PTEN Contributes to Embryonic Development and Tumor Suppression

Cancer Science, EarlyView.
Mice expressing only mutant PTEN Y138L, a protein which shows normal suppression of cellular AKT yet lacks protein phosphatase activity, die in utero, and heterozygous mice display a range of tumors. This indicates both the lipid and protein phosphatase activities of PTEN work together for normal tumor suppression and embryonic development.
Priyanka Tibarewal, Laura Spinelli, Nisha Kriplani, Helen Wise, Nadege Poncet, Giulia Marzano, Karen E. Anderson, Katarzyna M. Grzes, Zofia Varyova, Mahreen Adil, C. Peter Downes, Phillip T. Hawkins, Len R. Stephens, Kate G. Storey, Doreen A. Cantrell, Bart Vanhaesebroeck, Nicholas R. Leslie   +16 more
wiley   +1 more source

Elevated concentration of TNF-a induces trophoblast differentiation in mouse blastocyst outgrowths

, 2003
Elevated expression of tumour necrosis factora\ud (TNF-a) is associated with adverse pregnancy outcome.\ud This study has examined the expression of TNF-a and its\ud receptors (TNF-Rs) by mouse blastocysts and blastocyst\ud outgrowths from day 4 to 9.5 ...
Harvey, Mark, Herington, Adrian, Teh, Alison, Boucaut, Kerry, Whiteside, Eliza, Garcia-Aragon, Juanita   +5 more
core   +1 more source

Biallelic Truncating Variant in LRGUK Is Associated With Severe Multiple Morphological Abnormalities of the Sperm Flagella and Sperm Nuclear Defects in Humans

Clinical Genetics, EarlyView.
A homozygous truncating variant in LRGUK results in loss of the LRGUK protein and causes multiple morphological abnormalities of the flagella (MMAF), disrupted axonemal architecture, central pair defects, abnormal chromatin organization, and severe male infertility, establishing LRGUK as a novel human infertility gene.
Wiâme Mokkedem, Zeina Wehbe, Anne‐Laure Barbotin, Angèle Boursier, Célia Tebbakh, Nicolas Thierry‐Mieg, Marie Bidart, Antonella Migliaccio, Rosanna Chianese, Christophe Arnoult, Raoudha Zouari, Zine‐Eddine Kherraf, Pierre F. Ray, Guillaume Martinez, Charles Coutton   +14 more
wiley   +1 more source

Blastocyst Implantation in Rats : A Morphological Approach

, 1998
Very complex cellular events take place at the morphological and molecular levels both in the maternal and embryonic sites during blastocyst implantation.
Basimo?lu-Koca Y., Bulut H.E., Gursoy E.
core   +1 more source

Human blastocyst biopsy and vitrification

, 2019
Blastocyst biopsy is performed to obtain a reliable genetic diagnosis during IVF cycles with preimplantation genetic testing. Then, the ideal workflow entails a safe and efficient vitrification protocol, due to the turnaround time of the diagnostic ...
Maggiulli, Roberta, Ubaldi, Filippo M, CIMADOMO, DANILO, Rienzi L., GIANCANI, ADRIANO, Rienzi, Laura, Maggiulli R., Ubaldi F. M.   +7 more
core   +1 more source

Ploidy‐Dependent Response to Anticancer Drugs of Human Embryonic Stem Cells

Cell Proliferation, EarlyView.
Using isogenic hESCs, differing only in their ploidy level, we show the effect of ploidy on the resistance to anticancer drugs. We demonstrate that polyploidy, by itself, confers sensitivity to chemotherapies, leading to higher apoptosis and delayed proliferation, while the loss of p53 reverses the phenotype, demonstrating higher resistance.
Guy Haim‐Abadi, Nissim Benvenisty
wiley   +1 more source