Results 91 to 100 of about 1,050,605 (265)

Artemisinin‐Loaded Core‐Shell Microspheres Modulate Histone Lactylation to Remodel the Microenvironment for Cartilage Protection in Rheumatoid Arthritis

Advanced Healthcare Materials, EarlyView.
ART@PLGA@GH‐A microspheres are developed via microfluidic technology for intra‐articular delivery of artemisinin. The system remodels rheumatoid arthritis microenvironments by regulating metabolic and inflammatory disorders, reducing lactate‐driven histone lactylation and cellular senescence, while restoring cartilage homeostasis and protecting joint ...
Shijie Meng, Jianxin Li, Yuyang Luo, Yisi Liu, Feng Xing, Yingchen Ni, Fengyuan Zhang, Zhicheng Fan, Hua Xu, Weidong Zhang, Youhua Wang   +10 more
wiley   +1 more source

Development of a quantification method to specific Anti-ORF2 antibody using a blocking ELISA

, 2007
Guillossou, S.; Lebon, E.; Mieli, L.; Bonnard, M.; Thomson, D.. (2007). Development of a quantification method to specific Anti-ORF2 antibody using a blocking ELISA.
Mieli, L., Lebon, E., Bonnard, M., Thomson, D., Guillossou, S.   +4 more
core  

Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni

Memorias do Instituto Oswaldo Cruz, 1992
A new serological assay dot-dye-immunoassay (dot-DIA) was evaluated for the diagnosis of schistosomiasis mansoni. This method consist of four steps: (a) biding of antigens to a nitrocellulose membrane (NC); (b) blocking of free sites of the NC; (c ...
Ana Lúcia Teles Rabello, Emmanuel Dias Neto, Maria Monica Aguiar Garcia, Naftale Katz   +3 more
doaj   +1 more source

Bacteria‐Trained, MnO2‐Armored Dendritic Cells for Immunotherapy

Advanced Materials, EarlyView.
MnO2‐armored, bacteria‐trained dendritic cells undergo tumor‐responsive mechanical and immunological transformation. The nanocoating masks immune ligands and maintains a mechanically stiff, quiescent state during circulation, while the tumor microenvironment removes the armor and restores cellular deformability and immune accessibility.
Zhao Guo, Min Ge, Zesong Ruan, Tingwang Shi, Mahyar Mahmoudi, Soohwan An, Na Kong, Yunfeng Chen, Han Lin, Wei Tao   +9 more
wiley   +1 more source

Resolution of Unexpected IDEXX ELISA Positive Results for Antibodies Against Porcine Reproductive and Respiratory Syndrome Virus with IFA and A Blocking ELISA

, 2002
Ferrin, N.H.; Torremorell, M.; Gramer, M.; Fang, Y.; Murtaugh, M.P.; Johnson, C.R.; Nelson, E.A.. (2002). Resolution of Unexpected IDEXX ELISA Positive Results for Antibodies Against Porcine Reproductive and Respiratory Syndrome Virus with IFA and A ...
Johnson, C.R., Torremorell, M., Murtaugh, M.P., Nelson, E.A., Gramer, M., Ferrin, N.H., Fang, Y.   +6 more
core  

A Retinal Matrix Stiffening Model Reveals FAK‐Dependent Mechanotransduction in Pericytes Underlies Vascular Destabilization

Advanced Materials, EarlyView.
In this study, we show that retinal matrix stiffness is increased in diabetic mice. Using engineered human vascular models with controlled matrix stiffness, we demonstrate that stiffening activates focal adhesion kinase (FAK) signaling in pericytes, suppresses Notch signaling, and disrupts vascular morphology.
Emily Warren, Alex Wu, Lucas Ramirez, Yaqing Huang, Connor Amelung, Sophia Saxonhouse, Sharon Gerecht   +6 more
wiley   +1 more source

Defined Matrices Reveal Epithelial‐ and Mesenchymal‐Specific Responses to Stiffness and Adhesion in Human iPSC‐Derived Intestinal Organoids

Advanced Materials, EarlyView.
Defined PEG hydrogels enable human iPSC‐derived intestinal organoid culture without Matrigel. PEG‐ and Matrigel‐cultured organoids both support duodenal‐like epithelial identity, but direct comparison reveals subtle epithelial differentiation‐marker shifts and stronger mesenchymal ECM‐remodeling/integrin programs in PEG.
Alicja Kuziola, Tamara Fawzi, Emma H. Kromann, Marion Leleu, Joanna M. Drelinkiewicz, Dusko Ilic, Cécile A. Dreiss, Joana F. Neves, Eileen Gentleman   +8 more
wiley   +1 more source

ELISA

, 2015
Raw data form TGF-b activation performed by ELISA ...
Tania C. Araujo-Jorge (3322635), Antônio S. Gonçalves (3322632), Mariana C. Waghabi (158752), Leila Mendonça-Lima (319640), Patrícia M. Ferrão (319637), Luciana R. Garzoni (367277), Jean Jacques Feige (734539), Sabine Bailly (158749), Ana Paula Lima (734538), Wim M. Degrave (158743), Claudia M. d'Avila-Levy (225684)   +10 more
core   +1 more source

The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA

Brazilian Journal of Medical and Biological Research, 2000
F1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best
G.H.T.S. Barbosa, E.M. Santana, A.M.P. Almeida, A.M. Araujo, O. Fatibello-Filho, L.B. Carvalho Jr.   +5 more
doaj   +1 more source

An Engineered Thio‐Amino Polymer Achieves Potent, Safe, and Broad Cell Type Delivery in the Lung Mapped by Spatial Transcriptomics

Advanced Materials, EarlyView.
Synthesis and screening of a polymer library produced a versatile and inhalable mRNA vehicle for the lungs (P81). Compared to a published LNP, P81 provides increased delivery efficacy, achieving widespread expression in broad cell types, with no toxicity.
Pramodkumar EK, Younghun Jung, Elex S. Rose, Jae Yeon Joo, James R. Rose, José A. F. Assumpção, Hyeyoon Jeong, Lorena C. S. Chaves, Blake Andrew Miller, João Paulo Pera Mendes, Hannah E. Peck, Charmy J. Patel, Loren E. Sasser, Heather Pathak, Varun V. Mosur, Jaehyeon Hwang, Guilherme de Oliveira Lopes, Merrilee Thoresen, Santiago Cornejo, Peres Badial, Alicia Olivier, Amelia R. Woolums, Samuele Stazzoni, Noemi Manganaro, Eleonora Nencini, Giulio Pierleoni, Emanuele Andreano, Rino Rappuoli, Nichole Orr‐Burks, Sarah R. Hosking, Nicholas S. Heaton, Eric R. Lafontaine, Robert J. Hogan, Francois Villinger, Daryll Vanover, Chiara Zurla, Philip J. Santangelo   +36 more
wiley   +1 more source