Results 111 to 120 of about 1,050,605 (265)
This study reveals a copper‐driven mechanism of COPD acute exacerbations mediated by the TNF‐α/ATP7A axis. TNF‐α suppresses the copper transporter ATP7A via competitive NF‐κB/CREB1/CBP signaling, resulting in intracellular copper accumulation, mitochondrial oxidative stress, and epithelial barrier disruption.
Xinru Xiao +7 more
wiley +1 more source
Objective-To develop and apply the liquid-phase blocking sandwich ELISA (BLOCKING-ELISA) for the quantification of antibodies against foot-and-mouth disease virus (FMDV) strains O-1 Campos, A(24) Cruzeiro, and C-3 Indaial.Design-Antibody quantification ...
Montassier, H. J. +2 more
core +1 more source
Porcine deltacoronavirus (PDCoV) is an emerging enteric pathogen that poses a significant threat to the global swine industry and carries a potential public health risk.
Dexin Li +11 more
doaj +1 more source
Mechanical stress activates BAP31 in chondrocytes. BAP31 competes with ATG14 for binding to STX17, disrupting the STX17–ATG14 complex required for autophagosome–lysosome fusion. The resultant autophagic flux blockade drives the generation of autophagy‐derived exosomes, which mediate pathological cartilage calcification in OA. Chondrocyte‐targeted BAP31
Zhi‐hua Xu +13 more
wiley +1 more source
Sensitivity and specificity of the EB-ELISA.
Performance of the EB-ELISA with sera from guinea pigs immunized with different strains of EV71 or mice with different coxsackievirus. Sera were collected 14 days after the 2nd immunization and normalized to a virus neutralization titer of 16 before the ...
Xiao Fang Lim (285655) +8 more
core +1 more source
ЗНАЧЕНИЕ КОМПЛЕКСА МЕТОДОВ ЛАБОРАТОРНОЙ ДИАГНОСТИКИ В БОРЬБЕ С ЯЩУРОМ
A reliable combination of methods for detection of FMD virus antigen and its identification was developed. It is recommended to carry out seromonitoring in zones under threat and during FMD eradication campaigns using a liquid-phase blocking ELISA for ...
doaj
Establishment of an Indirect ELISA Method for the Detection of the Bovine Rotavirus VP6 Protein
The objective of this study was to develop an indirect ELISA utilizing a polyclonal antibody against bovine rotavirus (BRV) VP6 protein. To achieve this, pcDNA3.1-VP6, a recombinant eukaryotic expression plasmid, was constructed based on the sequence of ...
Xiaoxia Niu +9 more
doaj +1 more source
The adipocyte MKK3–p38β axis emerges as an adaptive stress‐response pathway during obesity. Human visceral adipose tissue analyses, adipocyte‐specific mouse models, and primary adipocytes show that the MKK3–p38β axis supports insulin responsiveness by restraining p70S6K‐dependent inhibitory IRS1 feedback and preserving AKT–GLUT4 signaling. This pathway
Edgar Bernardo +20 more
wiley +1 more source
Steverink, P.; Muller, K.; Fichtner, D.; Kaden, V.. (1999). Porcine Reproductive and Respiratory Syndrome Virus Serotype-distinguishing performance of the Immunoperoxidase Monolayer Assay and the double-Blocking ELISA.
Fichtner, D. +3 more
core
Development of a polyclonal competitive enzyme-linked immunosorbent assay for detection of antibodies to Ehrlichia ruminantium [PDF]
A polyclonal competitive enzyme-linked immunosorbent assay (PC-ELISA) is described for detection of antibodies to Ehrlichia (Cowdria) ruminantium by using a soluble extract of endothelial cell culture-derived E.
Sumption, Keith J +2 more
core

