Results 51 to 60 of about 1,050,605 (265)

Antibodies against bovine herpesvirus (BHV) 5 may be differentiated from antibodies against BHV1 in a BHV1 glycoprotein E blocking ELISA

, 2001
We examined whether antibodies against bovine herpesvirus (BHV) 5 cross-react with BHV1 antigens and whether they could interfere with BHV1 eradication programmes.
Oirschot, J.T., van, Mars, M.H., Wellenberg, G.J.   +2 more
core   +1 more source

Characterization of therapeutic antibodies targeting lymphatic and vascular resistance factor for the treatment of hypervascularized and highly metastatic cancers

Molecular Oncology, EarlyView.
LVRF (lymphatic and vascular resistance factor) is a VEGFA splice variant upregulated in response to anti‐angiogenic therapy that fosters tumor adaptation through enhanced vascularization, lymphangiogenesis, and metastatic dissemination. Targeting LVRF with fully human neutralizing antibodies disrupts these processes and limits resistant tumor growth ...
Marion David, Arthur Karaulic, Audrey Bennetot, Clémence Fournier, Quentin Odet, Arthur Gouraud, Isabelle Bourget, Cédric Gaggioli, Sandy Giuliano, Frédéric Luciano, Olivier Léger, Zaki Sellam, Gilles Pagès   +12 more
wiley   +1 more source

Establishment of an In-House Indirect Enzyme-Linked Immunosorbent Assay to Detect Antibodies Against African Horse Sickness Based on Monovalent and Polyvalent Live Attenuated Vaccines During the First Outbreak in Thailand

Animals
Standard serological tests post-vaccination, such as enzyme-linked immunosorbent assay (ELISA), complement fixation, and virus neutralization, are crucial for monitoring African horse sickness (AHS). However, the availability of commercial test kits such
Darsaniya Punyadarsaniya, Machimaporn Taesuji, Khate Rattanamas, Sakchai Ruenphet   +3 more
doaj   +1 more source

An ELISA protocol to improve the accuracy and reliability of serological antibody assays

MethodsX, 2017
To assay serum antibodies by indirect ELISA, it is critical to eliminate a variety of false positive and negative reactions attributed to the principle.
Takaki Waritani, Jessica Chang, Bonnie McKinney, Kuniaki Terato   +3 more
doaj   +1 more source

USP7 inhibits FOXO1 activity in chronic lymphocytic leukaemia cells enhancing cell survival

Molecular Oncology, EarlyView.
CLL cells rely on BCR‐mediated signalling for survival and proliferation in part through suppression of FOXO1. FOXO1 activity is regulated by a plethora of post‐translational modifications including phosphorylation and ubiquitination. We demonstrated that dual inhibition of BCR signalling and the deubiquitinase USP7 activates FOXO1, modulating its ...
Hassan N.B. Almuhanna, Jodie Hay, Michael W. Moles, Lucy Clarke, Jennifer Cassels, Alison Sefcick, Alison M. McCaig, Heather G. Jørgensen, Alison M. Michie   +8 more
wiley   +1 more source

Hydrophilic Polyelectrolyte Multilayers Improve the ELISA System: Antibody Enrichment and Blocking Free [PDF]

Polymers, 2017
In this study, polyelectrolyte multilayers were fabricated on a polystyrene (PS) plate using a Layer-by-Layer (LbL) self-assembly technique. The resulting functional platform showed improved performance compared with conventional enzyme-linked immunosorbent assay (ELISA) systems. Poly(diallyldimethylammonium chloride) (PDDA) and poly(acrylic acid) (PAA)
Lai, Xing, Gao, Gan, Watanabe, Junji, Liu, Huiyu, Shen, Heyun   +4 more
openaire   +2 more sources

Non-specific adsorption of fish immunoglobulin M (IgM) to blocking reagents on ELISA plate wells [PDF]

, 2007
Enzyme-linked immunosorbent assay (ELISA) is a popular technique for quantifiable detection of specific antibodies in warm-blooded animals, but it has not been accepted for detection of fish antibodies because of its low reproducibility, which is due in ...
Yoshimizu, M., Kim, W.S., Nishizawa, T.
core   +1 more source

Establishment of an indirect ELISA method for detection of human cytomegalovirus [PDF]

精准医学杂志
Objective To establish an indirect ELISA method for the detection of human cytomegalovirus (HCMV) based on HCMV IE1/pp65/pp150 recombinant protein. Methods From June to December 2024, 5 serum samples from HCMV IgG-positive patients, 50 serum samples from
LI Xu, WANG Shan, HUANG Huan, WANG Bin, QIAN Dongmeng
doaj   +1 more source

Peripheral Neutrophil Activation and Extracellular Trap Formation in Amyotrophic Lateral Sclerosis

Annals of Clinical and Translational Neurology, EarlyView.
Markers of neutrophil activation are increased in plasma during ALS, and markers of NET formation associate with ALS survival. ABSTRACT Objectives Peripheral neutrophil levels in amyotrophic lateral sclerosis (ALS) inversely correlate with survival, suggesting a role for neutrophils in disease progression.
Lillia A. Baird, Haley McQuown, Jihyun Park, Samuel J. Teener, Ian F. Webber‐Davis, Andrew D. Carter, Dae‐Gyu Jang, Eva L. Feldman, Stephen A. Goutman, Benjamin J. Murdock   +9 more
wiley   +1 more source

Results of B-ELISA, I-ELISA and SNT with reference sera.

, 2013
Note: Coincidence rates between different methods were calculated using Microsoft Excel's CORREL function. The results showed that the blocking ELISA and the SNT have a positive coincidence with coincidence rate of 70.6%.
Lei Yu (70258), Guoxin Li (291236), Qiaoyang Teng (291237), Zejun Li (176523), Xuesong Li (291235), Xiaogang Wu (115894)   +5 more
core   +1 more source