Results 41 to 50 of about 1,050,605 (265)

Improving an anti-beta2 GPI ELISA by reducing the influence of a blocking agent [PDF]

Arthritis Research & Therapy, 2001
There are still considerable interlaboratory differences in positivity rate in anti-β2GPI ELISA. We have already shown that BSA as ablocking agent could introduce a substantial interference effect in an anti-β2GPI ELISA. The aim of this study was to validate and possibly reduce an interference effect of different blocking agents on the detection of ...
Ambrozic, A, Bozic, B, Hojnik, M, Kveder, T   +3 more
openaire   +1 more source

Development of a Blocking ELISA for Screening Antibodies to Porcine Rubulavirus, La Piedad Michoacan Virus

, 1999
A blocking enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to porcine rubulavirus (La Piedad Michoacan Virus [LPMV]) in serum samples from pigs.
J. Moreno-Lopez, F. McNeilly, M. Merza, P. Hernandez, G. Allan, A. Bergvall, M. Svenda, A. Nordengrahn   +7 more
core   +1 more source

Direct replacement of antibodies with molecularly imprinted polymer (MIP) nanoparticles in ELISA - development of a novel assay for vancomycin [PDF]

, 2013
A simple and straightforward technique for coating microplate wells with molecularly imprinted polymer nanoparticles (nanoMIPs) to develop ELISA type assays is presented here for the first time.
Guerreiro, Antonio R., Piletska, Elena V., Moczko, Ewa, Chianella, Iva, Caygill, J. S., Piletsky, Sergey A., Perez De Vargas Sansalvador, Isabel M., Whitcombe, Michael J.   +7 more
core   +1 more source

Optimisation of indirect elisa by comparison of different antigen preparations for detection of antibodies against schmallenberg virus [PDF]

Kafkas Universitesi Veteriner Fakültesi Dergisi, 2020
Schmallenberg virus (SBV) infection, discovered in 2011, was reported in Europe including Turkey, Africa and recently in some Asian countries.
Ahmet Kursat AZKUR, Emel AKSOY, Murat YILDIRIM, Kader YILDIZ   +3 more
doaj   +1 more source

Neutralization of the PTX3/CD44 axis suppresses TGF‐β1 signaling in kidney fibrosis

FEBS Letters, EarlyView.
This study investigates how the PTX3/CD44 axis promotes kidney fibrosis by activating TGF‐β1 signaling. We demonstrate that the neutralizing antibody WHC‐001 effectively inhibits this pathway, suppressing myofibroblast activation and partial epithelial–mesenchymal transition (EMT).
Chao‐Chun Cheng, Shen‐Shin Chang, Feng‐Wei Chen, Ying‐Chieh Su, Thanh Phuong Nguyen, Yu‐Wei Hsiao, Jhih‐Ying Chi, Hsin‐Yin Liang, Ju‐Ming Wang   +8 more
wiley   +1 more source

ELISA

, 2022
 origin data of ELISA 
Lai Jiang (12959588)
core   +2 more sources

Solid‐phase extraction treatment is required for measurement of active glucagon‐like peptide‐1 by enzyme‐linked immunosorbent assay kit affected by heterophilic antibodies

Journal of Diabetes Investigation, 2019
Aims/Introduction It is reported that interfering substances in the blood might influence the value for measurement of active glucagon‐like peptide‐1 (GLP‐1) in human plasma.
Tomonori Hasegawa, Miho Komagata, Akihiro Hamasaki, Norio Harada, Yutaka Seino, Nobuya Inagaki   +5 more
doaj   +1 more source

Identification and elimination of false-positives in an ELISA-based system for qualitative assessment of glycoconjugate binding using a selection of plant lectins

BioTechniques, 2007
Systematic optimization of a lectin-based enzyme-linked immunosorbent assay (ELISA) procedure using a panel of 21 biotinylated plant lectins and a glycoprotein with defined glycosylation (i) identified blocking as a limiting step in solid phase sugar ...
Babak Afrough, Miriam V. Dwek, Pamela Greenwell   +2 more
doaj   +1 more source

Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy

FEBS Letters, EarlyView.
The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley   +1 more source

The use of an indirect ELISA with protein G-peroxidase conjugate and a blocking ELISA to demonstrate recent bluetongue infection in sheep

Journal of Virological Methods, 1994
The humoral immune response of sheep infected with bluetongue virus serotypes 3, 9 and 16 was monitored by plaque inhibition (PI), blocking ELISA (BELISA) and indirect ELISA over a period of 63 days post-infection. Results indicated that testing of a single plasma or serum sample by both a BELISA and an indirect ELISA using a recombinant streptococcal ...
Lunt, R, Blacksell, S, Newberry, K
openaire   +3 more sources