Results 21 to 30 of about 1,050,605 (265)

Validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich Elisa [PDF]

Ветеринария сегодня, 2018
The paper describes the process of the validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich ELISA for FMD types A, 0 and Asial.
T. K. Mayorova, Ye. N. Kalinina, O. V. Vorobyova, N. N. Lugovskaya, K. S. Maikova, G. M. Goryacheva   +5 more
core   +2 more sources

A novel biotinylated nanobody-based blocking ELISA for the rapid and sensitive clinical detection of porcine epidemic diarrhea virus. [PDF]

J Nanobiotechnology, 2019
Background Porcine epidemic diarrhea virus (PEDV), which is characterized by severe watery diarrhea, vomiting, dehydration and a high mortality rate in piglets, leads to enormous economic losses to the pork industry and remains a large challenge ...
Ma Z, Wang T, Li Z, Guo X, Tian Y, Li Y, Xiao S.   +6 more
europepmc   +2 more sources

Preparation of Monoclonal Antibodies Against Porcine Circovirus Type 2 Capsid Protein and Development of a Blocking ELISA for Detection of the Antibody Against the Virus. [PDF]

Vet Sci
Porcine circovirus type 2 (PCV2) is the primary causative agent of a spectrum of porcine circovirus-associated diseases (PCVDs) and remains a major threat to the global swine industry.
Sun H, Liu Q, Zhai S, Wu B, Ma Z, Sun Y, Ye K, Wang H, Gao Y, Wang X, Bai J, Jiang P.   +11 more
europepmc   +2 more sources

Development and application of a blocking ELISA method based on Cap protein for detecting antibodies against porcine circovirus 2. [PDF]

Microbiol Spectr
Porcine circovirus type 2 (PCV2) serves as the key pathogen linked to porcine circovirus-associated disease, representing a considerable risk to the worldwide swine industry.
Liu Q, Gao S, Li J, Yang J, Zhu Y, Zhu J, Zhou Y, Shan T, Tong W, Zheng H, Kong N, Jiang Y, Liu C, Tong G, Yu H.   +14 more
europepmc   +2 more sources

Establishment of a Blocking ELISA Detection Method for Against African Swine Fever Virus p30 Antibody. [PDF]

Front Vet Sci, 2021
African swine fever (ASF) is a highly lethal hemorrhagic viral disease of domestic pigs caused by African swine fever virus (ASFV). A sensitive and reliable serological diagnostic assay is required, so laboratories can effectively and quickly detect ASFV
Yu X, Zhu X, Chen X, Li D, Xu Q, Yao L, Sun Q, Ghonaim AH, Ku X, Fan S, Yang H, He Q.   +11 more
europepmc   +2 more sources

Development and application of a monoclonal antibody-based blocking ELISA for detection of antibodies to Tembusu virus in multiple poultry species. [PDF]

BMC Vet Res, 2018
Background Tembusu virus (TMUV) is a member of the genus Flavivirus. Outbreak of this virus infection in duck flocks was first observed in China in April 2010, causing severe egg drop and neurological signs in laying ducks.
Zhang L, Li Z, Jin H, Hu X, Su J.
europepmc   +2 more sources

Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65. [PDF]

J Vet Med Sci, 2016
Mycoplasma hyopneumoniae causes porcine enzootic pneumonia, an economically important disease of swine. A more sensitive and reliable method for detection of serum antibodies is needed for epidemiological investigations and to evaluate the effect of ...
Liu M, DU G, Zhang Y, Wu Y, Wang H, Li B, Bai Y, Feng Z, Xiong Q, Bai F, Browning GF, Shao G.   +11 more
europepmc   +2 more sources

Development of epitope-blocking ELISA for universal detection of antibodies to human H5N1 influenza viruses. [PDF]

PLoS ONE, 2009
BACKGROUND: Human infections with highly pathogenic H5N1 avian influenza viruses have generally been confirmed by molecular amplification or culture-based methods. Serologic surveillance has potential advantages which have not been realized because rapid
Mookkan Prabakaran, Hui-Ting Ho, Nayana Prabhu, Sumathy Velumani, Milene Szyporta, Fang He, Kwai-Peng Chan, Li-Mei Chen, Yumiko Matsuoka, Ruben O Donis, Jimmy Kwang   +10 more
doaj   +1 more source

Enzyme-Linked Immunosorbent Assays with High Sensitivity for Antigen-Specific and Total Murine IgE: A Useful Tool for the Study of Allergies in Mouse Models

Allergology International, 2009
Background: In studies on allergies in mouse models, IgE production is an essential parameter to be evaluated. Here, we examine the effect of commercially available immunoreaction enhancer solutions and different blocking reagents in enzyme-linked ...
Toshiro Takai, Yuri Ochiai, Saori Ichikawa, Emi Sato, Takasuke Ogawa, Tomoko Tokura, Takatoshi Kuhara, Hiroshi Kawai, Hideki Hatanaka, Seizo Takahashi, Hideoki Ogawa, Ko Okumura   +11 more
doaj   +1 more source

Sensitivity and specificity for African horse sickness antibodies detection using monovalent and polyvalent vaccine antigen-based dot blotting [PDF]

Veterinary World, 2022
Background and Aim: The immune responses of animals infected with African horse sickness (AHS) virus are determined by enzyme-linked immunosorbent assay (ELISA), complement fixation, and virus neutralization test. During the outbreaks of AHS in Thailand,
Machimaporn Taesuji, Khate Rattanamas, Usakorn Kulthonggate, Thanongsak Mamom, Sakchai Ruenphet   +4 more
doaj   +1 more source