Results 11 to 20 of about 1,050,605 (265)

PENGEMBANGAN TEKNIK ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) MENGGUNAKAN ANTIBODI MONOKLONAL UNTUK MENDETEKSI ANTIBODI PENYAKIT BOVINE EPHEMERAL FEVER [PDF]

Jurnal Kedokteran Hewan, 2015
Penelitian ini bertujuan mengembangkan teknik enzyme-linked immunosorbent assay (ELISA) untuk mendeteksi antibodi terhadap virus bovine ephemeral fever (BEF). Pada penelitian ini dikembangkan uji ELISA langsung (direct ELISA) dan tidak langsung (indirect
Saepulloh, Muharam, R.M. Abdul Adjid, Atik Ratnawati, Indrawati Sendow, Sendow, Indrawati, Ratnawati, Atik, Muharam Saepulloh, Adjid, R.M. Abdul   +7 more
core   +3 more sources

A Blocking ELISA for the Detection of Specific Antibodies to Bovine Respiratory Syncytial Virus [PDF]

Journal of Veterinary Diagnostic Investigation, 1989
A blocking enzyme-linked immunosorbent assay (ELISA) has been adapted to detect specific antibodies in bovine sera to respiratory syncytial virus using a horseradish peroxidase-labeled monclonal antibody to the fusion protein of the virus. This assay plus an indirect blocking ELISA and indirect ELISA were used to detect antibodies to the bovine ...
Rhodes, Marvin B., Klucas, Carol A., Frey, Merwin L., Anderson, Gary A.   +3 more
core   +5 more sources

Evaluation of a Blocking ELISA Using a Urease Conjugate for the Detection of Antibodies to Pseudorabies Virus [PDF]

Journal of Veterinary Diagnostic Investigation, 2000
A blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions. A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA
Echeverría, María Gabriela, Nosetto, Edgardo Omar, Etcheverrigaray, María Elisa   +2 more
openaire   +6 more sources

Development of a sensitive and specific epitope-blocking ELISA for universal detection of antibodies to human enterovirus 71 strains. [PDF]

PLoS ONE, 2013
BACKGROUND: Human Enterovirus 71 (EV71) is a common cause of hand, foot and mouth disease (HFMD) in young children. It is often associated with severe neurological diseases and mortalities in recent outbreaks across the Asia Pacific region.
Yunrui Tan, Manli Tang, Kattur Venkatachalam Ashok Raj, Qingfeng Chen, Xiao Fang Lim, Tanja K Kiener, Vincent T K Chow, Jimmy Kwang, Fang He   +8 more
core   +2 more sources

Evaluation of a blocking ELISA for the detection of antibodies against Lawsonia intracellularis in pig sera [PDF]

Acta Veterinaria Scandinavica, 2011
Lawsonia intracellularis is a common cause of chronic diarrhoea and poor performance in young growing pigs. Diagnosis of this obligate intracellular bacterium is based on the demonstration of the microbe or microbial DNA in tissue specimens or faecal samples, or the demonstration of L. intracellularis-specific antibodies in sera. The aim of the present
Merza Malik, Nordengrahn Ann, Wallgren Per, Jacobson Magdalena, Emanuelson Ulf   +4 more
openaire   +4 more sources

Development of a Monoclonal Blocking ELISA for the Detection of Antibodies Against Fowlpox Virus

Journal of Veterinary Medicine, Series B, 2002
To provide a fast and easy method to detect antibodies against fowlpox virus (FWPV) particularly in high numbers of chicken sera we established a monoclonal blocking enzyme‐linked immunosorbent assay (ELISA). We chose two different monoclonal antibodies (mAb), anti‐FWPV 3D9/2B3 and anti‐FWPV 8F3/2E11, which are both directed against the 39‐kDa protein ...
Isa, G., Pfister, K., Kaaden, O. R., Czerny, Claus-Peter   +3 more
openaire   +4 more sources

Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animals [PDF]

mSphere, 2023
The global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses a significant threat to public health. Besides humans, SARS-CoV-2 can infect several animal species.
Yuan F, Chen C, Covaleda LM, Martins M, Reinhart JM, Sullivan DR, Diel DG, Fang Y.   +7 more
europepmc   +3 more sources

A blocking ELISA based on virus-like nanoparticles chimerized with an antigenic epitope of ASFV P54 for detecting ASFV antibodies. [PDF]

Sci Rep, 2023
African swine fever virus (ASFV) is a highly lethal pathogen of domestic and wild pigs. Due to no vaccines or drugs available, early accurate diagnosis and eradication of infected animals are the most important measures for ASFV prevention and control ...
Huang C, Cao C, Xu Z, Lin Y, Wu J, Weng Q, Liu Z, Jin Y, Chen P, Hua Q.   +9 more
europepmc   +2 more sources

A Customized Novel Blocking ELISA for Detection of Bat-Origin Swine Acute Diarrhea Syndrome Coronavirus Infection. [PDF]

Microbiol Spectr, 2023
Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a newly discovered emerging alphacoronavirus. SADS-CoV shares over 90% genome sequence identity with bat alphacoronavirus HKU2.
Cao L, Kong X, Li X, Suo X, Duan Y, Yuan C, Zhang Y, Zheng H, Wang Q.   +8 more
europepmc   +2 more sources

Detection of PPMV-1 antibodies in pigeons using a blocking ELISA based on an NP-specific monoclonal antibody. [PDF]

Poult Sci
Pigeon paramyxovirus type 1 (PPMV-1) is a prevalent infectious agent causing Newcastle disease in pigeons, yet serological methods for detecting PPMV-1-specific antibodies remain limited. In this study, we developed a blocking enzyme-linked immunosorbent
Zhao Z, Huang J, Duan X, Meng S, Chen H, Li C, He J, Wei T.   +7 more
europepmc   +2 more sources