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The Southern Blot: An Update [PDF]
Molecular Biotechnology, 1994 This article is devoted to the recent advances made in the Southern blotting technique, which is used for the detection of gel-fractionated DNA molecules following transfer to a membrane. The latest practical improvements made to the techniques of Southern blotting, probe labeling, and hybridization are discussed.
Dennis W. Harris +2 more
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Western Blot Processing Optimization: The Perfect Blot
2021Western blot processing is a well-established procedure that includes protein extraction from tissues and cells, gel electrophoresis separation, transfer to a membrane, and immunodetection with specific antibodies. Here, we show that optimization of washing helps to maximize the specific interactions of antigens and antibodies.
David P. Chimento +2 more
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2020
TheblotisasigninPeirce’sdiagrammaticsyntaxofexistentialgraphs that has hitherto been neglected in the literature on logical graphs. It is needed in order to trigger the cut-as-negation to come out from the scroll, namely from the implicational sign of a positive implicational (paradisiacal) logic.
Ahti-Veikko Pietarinen +4 more
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TheblotisasigninPeirce’sdiagrammaticsyntaxofexistentialgraphs that has hitherto been neglected in the literature on logical graphs. It is needed in order to trigger the cut-as-negation to come out from the scroll, namely from the implicational sign of a positive implicational (paradisiacal) logic.
Ahti-Veikko Pietarinen +4 more
openaire +2 more sources
Methods, 2006
Western blotting (protein blotting or immunoblotting) is a powerful and important procedure for the immunodetection of proteins post-electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly.
Biji T, Kurien, R Hal, Scofield
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Western blotting (protein blotting or immunoblotting) is a powerful and important procedure for the immunodetection of proteins post-electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly.
Biji T, Kurien, R Hal, Scofield
openaire +2 more sources
Protein blotting with direct blotting electrophoresis
Analytical Biochemistry, 1988Direct blotting electrophoresis, a method designed to be of general application for the separation and electroblotting of macromolecules, has been adapted to produce protein blots suitable for subsequent processing by standard techniques such as dye staining or immunological detection.
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Current Protocols in Immunology, 1993
AbstractSouthern blotting is the transfer of DNA fragments from an electrophoresis gel to a membrane support, resulting in immobilization of the DNA fragments, so the membrane carries a semipermanent reproduction of the banding pattern of the gel. After immobilization, the DNA can be subjected to hybridization analysis, enabling bands with sequence ...
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AbstractSouthern blotting is the transfer of DNA fragments from an electrophoresis gel to a membrane support, resulting in immobilization of the DNA fragments, so the membrane carries a semipermanent reproduction of the banding pattern of the gel. After immobilization, the DNA can be subjected to hybridization analysis, enabling bands with sequence ...
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2015
Western blotting is a technique that involves the separation of proteins by gel electrophoresis, their blotting or transfer to a membrane, and selective immunodetection of an immobilized antigen. This is an important and routine method for protein analysis that depends on the specificity of antibody-antigen interaction and is useful for the qualitative
Thomas S. Hnasko, Robert Hnasko
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Western blotting is a technique that involves the separation of proteins by gel electrophoresis, their blotting or transfer to a membrane, and selective immunodetection of an immobilized antigen. This is an important and routine method for protein analysis that depends on the specificity of antibody-antigen interaction and is useful for the qualitative
Thomas S. Hnasko, Robert Hnasko
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2003
Northern blotting is the technique whereby RNA molecules are denatured, fractionated through agarose on the basis of size, and then transferred to a solid matrix for subsequent hybridization to a specific labeled probe. The technique can be used to analyze the pattern of transgene expression in an organism, and changes in that pattern following ...
J W, Pollard, C R, Perry, C F, Thurston
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Northern blotting is the technique whereby RNA molecules are denatured, fractionated through agarose on the basis of size, and then transferred to a solid matrix for subsequent hybridization to a specific labeled probe. The technique can be used to analyze the pattern of transgene expression in an organism, and changes in that pattern following ...
J W, Pollard, C R, Perry, C F, Thurston
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2009
Slice blotting is a technique for recording the spatial distribution of extracellular signaling molecules released from thin slices of living tissue. Slices are positioned on the surface of a membrane that can trap secreted substances diffusing from the tissue. The pattern of membrane-bound antigens is subsequently visualized by immunoblotting.
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Slice blotting is a technique for recording the spatial distribution of extracellular signaling molecules released from thin slices of living tissue. Slices are positioned on the surface of a membrane that can trap secreted substances diffusing from the tissue. The pattern of membrane-bound antigens is subsequently visualized by immunoblotting.
openaire +3 more sources