Results 301 to 310 of about 1,441,912 (353)
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2015
Centrifuge blotting is an efficient and convenient method for elution and transfer of proteins from a polyacrylamide gel onto a polyvinylidene difluoride (PVDF) membrane by centrifugation.
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Centrifuge blotting is an efficient and convenient method for elution and transfer of proteins from a polyacrylamide gel onto a polyvinylidene difluoride (PVDF) membrane by centrifugation.
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2007
Western blotting is one of the most commonly used biochemical techniques to detect a specific protein from a mixture of proteins such as tissue extracts. Antibodies to the specific antigen are used to detect the protein. The mixture of proteins is resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a membrane.
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Western blotting is one of the most commonly used biochemical techniques to detect a specific protein from a mixture of proteins such as tissue extracts. Antibodies to the specific antigen are used to detect the protein. The mixture of proteins is resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a membrane.
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2009
Slice blotting is a technique for recording the spatial distribution of extracellular signaling molecules released from thin slices of living tissue. Slices are positioned on the surface of a membrane that can trap secreted substances diffusing from the tissue. The pattern of membrane-bound antigens is subsequently visualized by immunoblotting.
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Slice blotting is a technique for recording the spatial distribution of extracellular signaling molecules released from thin slices of living tissue. Slices are positioned on the surface of a membrane that can trap secreted substances diffusing from the tissue. The pattern of membrane-bound antigens is subsequently visualized by immunoblotting.
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Detection of Damage-Activated Metacaspase Activity by Western Blot in Plants.
Methods in molecular biology, 2022S. Stael +3 more
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2013
Western analysis of apolipoproteins, lipoproteins, and proteins involved in lipoprotein metabolism can be challenging due to their size, hydrophobic nature, and, in some cases, low abundance. Here we describe a Western blotting method that has been used successfully for many proteins involved in lipoprotein metabolism, as well as intact LDL or HDL ...
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Western analysis of apolipoproteins, lipoproteins, and proteins involved in lipoprotein metabolism can be challenging due to their size, hydrophobic nature, and, in some cases, low abundance. Here we describe a Western blotting method that has been used successfully for many proteins involved in lipoprotein metabolism, as well as intact LDL or HDL ...
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Molecular Biotechnology, 1994
The different steps involved in protein (Western) blotting and subsequent analysis of the proteins are reviewed. Electrophoretic separation of proteins, procedures of transfer to membranes, immunological and nonimmunological protein detection systems, and characterization of protein-nucleic acid and protein-protein interactions are described.
D, Egger, K, Bienz
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The different steps involved in protein (Western) blotting and subsequent analysis of the proteins are reviewed. Electrophoretic separation of proteins, procedures of transfer to membranes, immunological and nonimmunological protein detection systems, and characterization of protein-nucleic acid and protein-protein interactions are described.
D, Egger, K, Bienz
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Analytical Chemistry, 2010
We develop a novel method for Western blot based on microfluidics, incorporating the internal molecular weight marker, loading control, and antibody titration in the same protocol. Compared with the conventional method which could detect only one protein, the microfluidic Western blot could analyze at least 10 proteins simultaneously from a single ...
Wenying, Pan, Wei, Chen, Xingyu, Jiang
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We develop a novel method for Western blot based on microfluidics, incorporating the internal molecular weight marker, loading control, and antibody titration in the same protocol. Compared with the conventional method which could detect only one protein, the microfluidic Western blot could analyze at least 10 proteins simultaneously from a single ...
Wenying, Pan, Wei, Chen, Xingyu, Jiang
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2009
The ''Rainbow western'' method permits detection of multiple antigens on a single protein blot. The procedure utilizes horseradish peroxidase (HRPase)-based detection with both a chemiluminescent and colorimetric substrate. In the ''Rainbow western'' procedure four different HRPase-colorimetric substrates that produce black, brown, red, and green ...
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The ''Rainbow western'' method permits detection of multiple antigens on a single protein blot. The procedure utilizes horseradish peroxidase (HRPase)-based detection with both a chemiluminescent and colorimetric substrate. In the ''Rainbow western'' procedure four different HRPase-colorimetric substrates that produce black, brown, red, and green ...
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2003
Western blotting (reviewed in 1-3; see also this vol., Chapter 24 ) refers to formation and detection of an antibody-antigen complex between an antibody and a polypeptide that is immobilized on derivatized paper.
S H, Kaufmann, J H, Shaper
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Western blotting (reviewed in 1-3; see also this vol., Chapter 24 ) refers to formation and detection of an antibody-antigen complex between an antibody and a polypeptide that is immobilized on derivatized paper.
S H, Kaufmann, J H, Shaper
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2003
Using slot blots, RNA can be applied, unfractionated, to a solid matrix. Slot blotting can be used as a rapid method for analyzing changes in transgene RNA quantity following developmental or physiological changes. However, it can only be applied to animals that bear a transgene that has hybridizable segments with little or no homology to host RNAs ...
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Using slot blots, RNA can be applied, unfractionated, to a solid matrix. Slot blotting can be used as a rapid method for analyzing changes in transgene RNA quantity following developmental or physiological changes. However, it can only be applied to animals that bear a transgene that has hybridizable segments with little or no homology to host RNAs ...
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