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Molecular Biotechnology, 1994
The different steps involved in protein (Western) blotting and subsequent analysis of the proteins are reviewed. Electrophoretic separation of proteins, procedures of transfer to membranes, immunological and nonimmunological protein detection systems, and characterization of protein-nucleic acid and protein-protein interactions are described.
D, Egger, K, Bienz
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The different steps involved in protein (Western) blotting and subsequent analysis of the proteins are reviewed. Electrophoretic separation of proteins, procedures of transfer to membranes, immunological and nonimmunological protein detection systems, and characterization of protein-nucleic acid and protein-protein interactions are described.
D, Egger, K, Bienz
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Analytical Chemistry, 2010
We develop a novel method for Western blot based on microfluidics, incorporating the internal molecular weight marker, loading control, and antibody titration in the same protocol. Compared with the conventional method which could detect only one protein, the microfluidic Western blot could analyze at least 10 proteins simultaneously from a single ...
Wenying, Pan, Wei, Chen, Xingyu, Jiang
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We develop a novel method for Western blot based on microfluidics, incorporating the internal molecular weight marker, loading control, and antibody titration in the same protocol. Compared with the conventional method which could detect only one protein, the microfluidic Western blot could analyze at least 10 proteins simultaneously from a single ...
Wenying, Pan, Wei, Chen, Xingyu, Jiang
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2009
The ''Rainbow western'' method permits detection of multiple antigens on a single protein blot. The procedure utilizes horseradish peroxidase (HRPase)-based detection with both a chemiluminescent and colorimetric substrate. In the ''Rainbow western'' procedure four different HRPase-colorimetric substrates that produce black, brown, red, and green ...
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The ''Rainbow western'' method permits detection of multiple antigens on a single protein blot. The procedure utilizes horseradish peroxidase (HRPase)-based detection with both a chemiluminescent and colorimetric substrate. In the ''Rainbow western'' procedure four different HRPase-colorimetric substrates that produce black, brown, red, and green ...
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2017
The Western blot is an important laboratory technique that allows for specific identification and characterization of proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins are electophoretically transferred to a polyvinylidene fluoride (PVDF) membrane which is then incubated with specific antibodies, then ...
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The Western blot is an important laboratory technique that allows for specific identification and characterization of proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins are electophoretically transferred to a polyvinylidene fluoride (PVDF) membrane which is then incubated with specific antibodies, then ...
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2003
Western blotting (reviewed in 1-3; see also this vol., Chapter 24 ) refers to formation and detection of an antibody-antigen complex between an antibody and a polypeptide that is immobilized on derivatized paper.
S H, Kaufmann, J H, Shaper
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Western blotting (reviewed in 1-3; see also this vol., Chapter 24 ) refers to formation and detection of an antibody-antigen complex between an antibody and a polypeptide that is immobilized on derivatized paper.
S H, Kaufmann, J H, Shaper
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2003
Using slot blots, RNA can be applied, unfractionated, to a solid matrix. Slot blotting can be used as a rapid method for analyzing changes in transgene RNA quantity following developmental or physiological changes. However, it can only be applied to animals that bear a transgene that has hybridizable segments with little or no homology to host RNAs ...
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Using slot blots, RNA can be applied, unfractionated, to a solid matrix. Slot blotting can be used as a rapid method for analyzing changes in transgene RNA quantity following developmental or physiological changes. However, it can only be applied to animals that bear a transgene that has hybridizable segments with little or no homology to host RNAs ...
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2012
Electrophoresis and the following western blot analysis are indispensable to investigate biochemical changes in cells and tissues exposed to nanoparticles or nanomaterials. Proteins should be extracted from the cells and tissues using a proper method, especially when phosphorylated proteins are to be detected.
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Electrophoresis and the following western blot analysis are indispensable to investigate biochemical changes in cells and tissues exposed to nanoparticles or nanomaterials. Proteins should be extracted from the cells and tissues using a proper method, especially when phosphorylated proteins are to be detected.
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A systematic approach to quantitative Western blot analysis
Analytical Biochemistry, 2020Lakshmi Pillai-Kastoori
exaly