Results 51 to 60 of about 1,558,933 (353)

ATF4‐mediated stress response as a therapeutic vulnerability in chordoma

Molecular Oncology, EarlyView.
We screened 5 chordoma cell lines against 100+ inhibitors of epigenetic and metabolic pathways and kinases and identified halofuginone, a tRNA synthetase inhibitor. Mechanistically halofuginone induces an integrated stress response, with eIF2alpha phosphorylation, activation of ATF4 and its target genes CHOP, ASNS, INHBE leading to cell death ...
Lucia Cottone, James Dunford, Eleanor Calcutt, Vicki Gamble, Filiz Senbabaoglu Aksu, Lorena Ligammari, Georgina Wherry, Giorgia Gaeta, John C. Christianson, Adrienne M. Flanagan, Udo Oppermann, Adam P. Cribbs   +11 more
wiley   +1 more source

Le Baratze (cercle de pierres) Méatsé 11 (commune d'Itxassou, Labourd. PA). [PDF]

Munibe Antropologia-Arkeologia, 1997
El Cromlech de Méatsé 11 presenta una arquitectura muy esmerada que contrasta con la ausencia de restos humanos, calcinados o no. Es muy parecido al círculo Méatsé 8 con el que es tangente, pero es posterior a él en el tiempo (fechado con el 14 C 2705 ...
Jacques Blot
doaj  

Les rapports entre société et eau : territorialité et/ou technicité [PDF]

, 2004
National audienceAfin d'appréhender la complexité des relations qui peuvent se tisser entre les sociétés et l'eau dans le cadre de la mise en oeuvre de nouvelles politiques de l'eau "territorialisantes", il s'agit de proposer une réflexion autour d ...
Blot, Frédérique
core   +1 more source

The novel mTOR inhibitor RAD001 (Everolimus) induces antiproliferative effects in human pancreatic neuroendocrine tumor cells [PDF]

, 2007
Background/Aim: Tumors exhibiting constitutively activated PI(3) K/Akt/mTOR signaling are hypersensitive to mTOR inhibitors such as RAD001 (everolimus) which is presently being investigated in clinical phase II trials in various tumor entities, including
Albert JM, Auernhammer CJ, Boulay A, Boulay A, Bousquet C, Burkhard Göke, Cao C, Christoph J. Auernhammer, Enrico N. De Toni, Faivre S, Gerald Spöttl, Grewe M, Heinrich H.D. Meyer, Hosoi H, Jeng YJ, Jennifer Meinecke, Kathrin Zitzmann, Kawamata S, Kirchner GI, Luo J, O?Reilly KE, Oberg K, Ramage JK, Sawyers CL, Shah T, Shaw RJ, Stephan Brand, Sun SY, Vignot S, von Wichert G, Wang L, Wanner K, Wendel HG, Wullschleger S, Yao JC, Yee KW   +35 more
core   +1 more source

Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma

Molecular Oncology, EarlyView.
Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken, Andrea Abel Gutierrez, Imke van Rossum, Cornelia G. Spruijt, Michiel Vermeulen, Guido van Mierlo, Blanca Scheijen, Annemiek B. van Spriel   +7 more
wiley   +1 more source

Study of protein expresion [sic] in peri-infarct tissue after cerebral ischemia [PDF]

, 2015
In this work, we report our study of protein expression in rat peri-infarct tissue, 48 h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal ...
Agulla, Jesús, Blanco, Miguel, Brea, David, Campos, Francisco, Castillo, José, Dávalos, Antoni, Gevaert, Kris, Ramos-Cabrer, Pedro, Sobrino, Tomás, Staes, An   +9 more
core   +5 more sources

PARP inhibition and pharmacological ascorbate demonstrate synergy in castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
Pharmacologic ascorbate (vitamin C) increases ROS, disrupts cellular metabolism, and induces DNA damage in CRPC cells. These effects sensitize tumors to PARP inhibition, producing synergistic growth suppression with olaparib in vitro and significantly delayed tumor progression in vivo. Pyruvate rescue confirms ROS‐dependent activity.
Nicolas Gordon, Peter T. Gallagher, Orly I. Richter, Neermala Poudel Neupane, Amy C. Mandigo, Jennifer J. McCann, Emanuela Dylgjeri, Irina Vasilevskaya, Christopher McNair, Channing J. Paller, Wm. Kevin Kelly, Karen E. Knudsen, Matthew J. Schiewer, Ayesha A. Shafi   +13 more
wiley   +1 more source

Functional compartmentalization of Rad9 and Hus1 reveals diverse assembly of the 9-1-1 complex components during the DNA damage response in Leishmania [PDF]

, 2016
The Rad9-Rad1-Hus1 (9-1-1) complex is a key component in the coordination of DNA damage sensing, cell cycle progression and DNA repair pathways in eukaryotic cells. This PCNA-related trimer is loaded onto RPA-coated single stranded DNA and interacts with
Damasceno, Jeziel D., McCulloch, Richard, Obonaga, Ricardo, Santos, Elaine V., Scott, Alan, Tosi, Luiz R.O.   +5 more
core   +1 more source

Western Blot (Tank-Blot) v1 [PDF]

, 2019
Blotting Buffer: 100mL 10x SDS running buffer 200mL 100% Methanol 700mL H20 Fill a tray with blotting buffer Transfer SDS gel to the blotting buffer Assemble blotting sandwich (take it, soak sponge and place on it, soak filter paper and place on it, place inverted SDS gel on top, without creating bubbles.Add charged PDVF membrane with forceps ...
openaire   +1 more source

Microfluidic Western blotting [PDF]

Proceedings of the National Academy of Sciences, 2012
Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe a Western blotting assay conducted in a single glass microchannel under purely electronic control. The μWestern blot is comprised of multiple steps: sample enrichment, protein sizing, protein immobilization (blotting), and in situ ...
Alex J, Hughes, Amy E, Herr
openaire   +2 more sources