Results 61 to 70 of about 842,305 (278)
Western Multi-blotting Device - Improve the Productivity of Protein Transfer [PDF]
, 2014 poster abstractDr. Stanley Chien from the Department of Electrical and Computer Engineering and Dr. Hiroki Yokota from Department of Biomedical Engineering jointly formed an interdisciplinary research team for the development of new devices to improve ...
Chien, Stanley, Yokota, Hiroki
core
CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein [PDF]
, 2019 The use of alternative promoters for the cell type-specific expression of a given mRNA/protein is a common cell strategy. NEMO is a scaffold protein required for canonical NF-κB signaling.
Babaei, Milad +8 more
core +1 more source
ATF4‐mediated stress response as a therapeutic vulnerability in chordoma
Molecular Oncology, EarlyView.We screened 5 chordoma cell lines against 100+ inhibitors of epigenetic and metabolic pathways and kinases and identified halofuginone, a tRNA synthetase inhibitor. Mechanistically halofuginone induces an integrated stress response, with eIF2alpha phosphorylation, activation of ATF4 and its target genes CHOP, ASNS, INHBE leading to cell death ...
Lucia Cottone +11 more
wiley +1 more source
Evidence for the existence of Dm0-like Lamin in Sf9 cells
浙江大学学报. 农业与生命科学版, 2015 The nuclear membrane of mammalian cells was composed of inner nuclear membrane, outer nuclear membrane and perinuclear space. The lamina was localized under the nucleoplasm face of inner nuclear membrane. It has been known that the lamina was distributed
Wei Wenqiang, Ji Shaoping, Zhang Yinyan
doaj +1 more source
Anales de la Facultad de Medicina, 2020 Introducción. La anisakidosis es una zoonosis causada por la ingestión accidental de larvas (L3) de anisákidos. Objetivo. Caracterizar el patrón proteico y perfil antigénico de la L3 de Anisakis simplex s.l. (tipo I), A. physeteris s.l.
Rosa Martínez-Rojas +3 more
doaj +1 more source
Measurement of autophagy via LC3 western blotting following DNA-damage-induced senescence
STAR Protocols, 2022 Summary: Senescent cells accumulation is associated with aging and age-related diseases, and recent findings suggest that autophagy, the activity of the intracellular degradation system, decreases during senescence.
Hitomi Yamamoto-Imoto +3 more
doaj +1 more source
, 2022 western blotting analysis of CD63,CD81,TSG101,calnexin,CD56,perforin and ...
openaire +1 more source
Giant FAZ10 is required for flagellum attachment zone stabilization and furrow positioning in Trypanosoma brucei [PDF]
, 2017 The flagellum and flagellum attachment zone (FAZ) are important cytoskeletal structures in trypanosomatids, being required for motility, cell division and cell morphogenesis.
Baqui, Munira M.A. +3 more
core +1 more source
Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma
Molecular Oncology, EarlyView.Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken +7 more
wiley +1 more source
Quantifying cytoskeletal protein interactions with far Western blotting
Frontiers in Molecular BiosciencesThe study of cytoskeletal proteins has been limited by some of their intrinsic properties, such as fixed intracellular localization and insolubility, which limit the implementation of many protein–protein interaction assays.
Marcelo M. Melo +4 more
doaj +1 more source