Results 41 to 50 of about 679 (168)

A construction strategy for a baculovirus‐silkworm multigene expression system and its application for coexpression of type I and type II interferons

MicrobiologyOpen, Volume 9, Issue 3, March 2020., 2020
In this study, we developed a construction strategy of recombinant Bombyx mori nucleopolyhedrovirus for multigene coexpression. This strategy provides a method for gene deletion or insertion at any site of the baculovirus genome in Escherichia coli.
Xingjian Liu, Xin Yang, Arslan Mehboob, Xiaoyuan Hu, Yongzhu Yi, Yinü Li, Zhifang Zhang   +6 more
wiley   +1 more source

Gene Expression Pattern and Regulatory Network of α‐Toxin Treatment in Bombyx mori

International Journal of Genomics, Volume 2019, Issue 1, 2019., 2019
Bacillus bombyseptieus is a pathogen of Bombyx mori; it can cause bacterial septicemia in silkworm. One of the components of the parasporal crystal toxin of B. bombyseptieus, α‐toxin, plays an important role in the process of infection in silkworm. In this study, we investigated the immune response of silkworm induced by α‐toxin by using RNA‐seq.
Tieshan Feng, Ping Lin, Jiao Gong, Dong Cheng, Xi Yang, Quan Zhang, Tingcai Cheng, Luigi Ceci   +7 more
wiley   +1 more source

PTP interacts with ORF1629 in BmNPV-infected cells.

, 2012
(A) A yeast two-hybrid screen was performed to identify interactions between BmNPV PTP and proteins in BmNPV-infected (12 h p.i.) BmN cells or in epidermal tissues from BmNPV-infected (2 d p.i.) 5th instar B. mori.
Susumu Katsuma (173041), Shizuo George Kamita (173055), Toru Shimada (12941), WonKyung Kang (173048), Yasue Koyano (173044), Ryuhei Kokusho (173052)   +5 more
core   +1 more source

Transcriptomic Analysis of Resistant and Susceptible Bombyx mori Strains Following BmNPV Infection Provides Insights into the Antiviral Mechanisms

International Journal of Genomics, Volume 2016, Issue 1, 2016., 2016
Purpose. To decipher transcriptomic changes and related genes with potential functions against Bombyx mori nucleopolyhedrovirus infection and to increase the understanding of the enhanced virus resistance of silkworm on the transcriptomic level. Methods.
Gang Li, Heying Qian, Xufang Luo, Pingzhen Xu, Jianhua Yang, Mingzhu Liu, Anying Xu, Kristina M. Herbert   +7 more
wiley   +1 more source

The Expression, Purification, and Characterization of a Ras Oncogene (Bras2) in Silkworm (Bombyx mori)

International Journal of Genomics, Volume 2013, Issue 1, 2013., 2013
The Ras oncogene of silkworm pupae (Bras2) may belong to the Ras superfamily. It shares 77% of its amino acid identity with teratocarcinoma oncogene 21 (TC21) related ras viral oncogene homolog‐2 (R‐Ras2) and possesses an identical core effector region. The mRNA of Bombyx mori Bras2 has 1412 bp. The open reading frame contains 603 bp, which encodes 200
Zhengbing Lv, Tao Wang, Wenhua Zhuang, Dan Wang, Jian Chen, Zuoming Nie, Lili Liu, Wenping Zhang, Lisha Wang, Deming Wang, Xiangfu Wu, Jun Li, Lian Qian, Yaozhou Zhang, Graziano Pesole   +14 more
wiley   +1 more source

Sensitivity of Polyvoltine Thai Strains of Bombyx mori to a BmNPV Isolate From Mahasarakham

, 2020
Virus infection by the Bombyx mori nucleopolyhedrovirus (BmNPV) is the most severe disease in Thai sericultural practice of polyvoltine silkworms. Here, we characterized a newly isolated BmNPV isolated from the Mahasarakham province in Thailand (BmNPV ...
Suraporn, Siripuk,, Terenius, Olle, Suraporn, Siripuk, Terenius, Olle,   +3 more
core   +1 more source

Use of Bacterial Artificial Chromosomes in Baculovirus Research and Recombinant Protein Expression: Current Trends and Future Perspectives

International Scholarly Research Notices, Volume 2012, Issue 1, 2012., 2012
The baculovirus expression system is one of the most successful and widely used eukaryotic protein expression methods. This short review will summarise the role of bacterial artificial chromosomes (BACS) as an enabling technology for the modification of the virus genome. For many years baculovirus genomes have been maintained in E.
Polly Roy, Rob Noad, M. Feiss, P. D. Ghiringhelli, T. Krishnan   +4 more
wiley   +1 more source

Detection of BmNPV in infected larvae and hemolymph.

, 2015
A, Silkworms in the control group at 120 h; red arrows point to the silkworms with apparent symptoms of BmNPV disease, and yellow arrows point to the silkworms without apparent symptoms of BmNPV disease. B, Silkworms in the experimental group at 120 h. C
Binbin Wang (161690), Kaizun Xu (693284), Hua Zhang (12549), Fashui Hong (280501), Weide Shen (589875), Bing Li (43671), Fanchi Li (589874), Min Ni (34723), Lie Ma (533809)   +8 more
core   +1 more source

RNAi Regulator C3PO Promotes Arbovirus Infection in Insect Vectors

Advanced Science, Volume 13, Issue 4, 19 January 2026.
RNA interference (RNAi) is vital for eukaryotes to defend against virus infection. The C3PO complex takes part in RNAi but its role in regulating viral infection remains unclear. This work finds that insect C3PO facilitates arbovirus replication by degrading precursors of miRNAs, especially miR‐971‐3p.
Yan Xiao, Tianyu Guan, Qianfeng Xia, Chen Chen, Qian Wang, Lan Luo, Hong Lu, Feng Cui   +7 more
wiley   +1 more source

Protection against Autoimmune Diabetes by Silkworm‐Produced GFP‐Tagged CTB‐Insulin Fusion Protein

Journal of Immunology Research, Volume 2011, Issue 1, 2011., 2011
In animals, oral administration of the cholera toxin B (CTB) subunit conjugated to the autoantigen insulin enhances the specific immune‐unresponsive state. This is called oral tolerance and is capable of suppressing autoimmune type 1 diabetes (T1D).
Qiaohong Meng, Wenfeng Wang, Xiaowen Shi, Yongfeng Jin, Yaozhou Zhang, Nick Giannoukakis   +5 more
wiley   +1 more source