Results 51 to 60 of about 693 (148)

Evaluation of Amplification Targets for the Specific Detection of Bordetella pertussis Using Real‐Time Polymerase Chain Reaction

Canadian Journal of Infectious Diseases and Medical Microbiology, Volume 25, Issue 4, Page 217-221, 2014., 2014
BACKGROUND: Bordetella pertussis infections continue to be a major public health challenge in Canada. Polymerase chain reaction (PCR) assays to detect B pertussis are typically based on the multicopy insertion sequence IS481, which offers high sensitivity but lacks species specificity. METHODS: A novel B pertussis real‐time PCR assay based on the porin
Mohammad Rubayet Hasan, Rusung Tan, Ghada N Al-Rawahi, Eva Thomas, Peter Tilley   +4 more
wiley   +1 more source

Laboratory-based surveillance of pertussis using multitarget real-time PCR in Japan: evidence for Bordetella pertussis infection in preteens and teens

New Microbes and New Infections, 2015
Between January 2013 and December 2014, we conducted laboratory-based surveillance of pertussis using multitarget real-time PCR, which discriminates among Bordetella pertussis, Bordetella parapertussis, Bordetella holmesii and Mycoplasma pneumoniae.
K. Kamachi, S. Yoshino, C. Katsukawa, N. Otsuka, Y. Hiramatsu, K. Shibayama   +5 more
doaj   +1 more source

Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum

International Journal of Bacteriology, Volume 2013, Issue 1, 2013., 2013
Objective. We compared laboratory developed real‐time PCR assays for detection of Mycoplasma hominis and for detection and differentiation of Ureaplasma urealyticum and parvum to culture using genitourinary specimens submitted for M. hominis and Ureaplasma culture. Methods.
Scott A. Cunningham, Jayawant N. Mandrekar, Jon E. Rosenblatt, Robin Patel, Sam R. Telford   +4 more
wiley   +1 more source

Detection of Bordetella pertussis from Clinical Samples by Culture and End‐Point PCR in Malaysian Patients

International Journal of Bacteriology, Volume 2013, Issue 1, 2013., 2013
Pertussis or whooping cough is a highly infectious respiratory disease caused by Bordetella pertussis. In vaccinating countries, infants, adolescents, and adults are relevant patients groups. A total of 707 clinical specimens were received from major hospitals in Malaysia in year 2011.
Tan Xue Ting, Rohaidah Hashim, Norazah Ahmad, Khairul Hafizi Abdullah, Sam R. Telford   +4 more
wiley   +1 more source

Molecular characterization of the BvgA response regulator of Bordetella holmesii

Microbiological Research, 2009
The BvgAS system controls the expression of most virulence factors in Bordetella pertussis. Recently, we identified an orthologous system in the related human pathogen Bordetella holmesii. However, while we found that the orthologous histidine kinases BvgS could be functionally exchanged between the two species, the B.
Horvat, Aleksandra, Gross, Roy
openaire   +2 more sources

Validation and Implementation of a Diagnostic Algorithm for DNA Detection of Bordetella pertussis, B. parapertussis, and B. holmesii in a Pediatric Referral Hospital in Barcelona, Spain [PDF]

, 2020
This study aimed to validate a comprehensive diagnostic protocol based on real-time PCR for the rapid detection and identification of Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii, as well as its implementation in the diagnostic
Jordán García, Iolanda, Godoy, Pere, Jane, Mireia, Valero-Rello, Ana, Henares, Desiree, Muñoz-Almagro, Carmen, Acosta, Lesly   +6 more
core   +3 more sources

Evaluation of real-time PCR for diagnosis of Bordetella pertussis infection

BMC Infectious Diseases, 2006
Background Nucleic acid amplification of the IS481 region by PCR is more sensitive than culture for detection and diagnosis of Bordetella pertussis but the assay has known cross-reactivity for Bordetella holmesii and its use as a routine diagnostic assay
Fox Julie D, Knorr Laina, Tilley Peter AG, Ahmed-Bentley Jasmine   +3 more
doaj   +1 more source

Characterization of a Highly Conserved Island in the Otherwise Divergent Bordetella holmesii and Bordetella pertussis Genomes [PDF]

Journal of Bacteriology, 2006
ABSTRACT The recently discovered pathogen Bordetella holmesii has been isolated from the airways and blood of diseased humans. Genetic events contributing to the emergence of B. holmesii are not understood, and its phylogenetic position among the bordetellae remains unclear.
Diavatopoulos, DA, Cummings, CA, van der Heide, HGJrivm, van Gent, Mrivm, Liew, S, Relman, DA, Mooi, FRrivm   +6 more
openaire   +3 more sources

Treatments affecting splenic function as a risk factor for valvular heart disease in Childhood Cancer Survivors: A DCCSS‐LATER study

Pediatric Blood &Cancer, Volume 71, Issue 11, November 2024.
Abstract Purpose Splenectomy might be a risk factor for valvular heart disease (VHD) in adult Hodgkin lymphoma survivors. As this risk is still unclear for childhood cancer survivors (CCS), the aim of this study is to evaluate the association between treatments affecting splenic function (splenectomy and radiotherapy involving the spleen) and VHD in ...
Bente M. Houtman, Iris Walraven, Livia Kapusta, Arco J. Teske, Eline van Dulmen‐den Broeder, Wim J. E. Tissing, Marry M. van den Heuvel‐Eibrink, A. B. Birgitta Versluys, Dorine Bresters, Margriet van der Heiden‐van der Loo, Cécile Ronckers, Wouter E. M. Kok, Helena J. H. van der Pal, Saskia M. F. Pluijm, Geert O. Janssens, Nicole M. A. Blijlevens, Leontien C. M. Kremer, Jacqueline J. Loonen, E. A. M. Lieke Feijen   +18 more
wiley   +1 more source

Identification and regulation of expression of a gene encoding a filamentous hemagglutinin-related protein in Bordetella holmesii

BMC Microbiology, 2007
Background Bordetella holmesii is a human pathogen closely related to B. pertussis, the etiological agent of whooping cough. It is able to cause disease in immunocompromised patients, but also whooping cough-like symptoms in otherwise healthy individuals.
Gross Roy, Beier Dagmar, Schmitt Karin, Link Stefanie   +3 more
doaj   +1 more source