Results 1 to 10 of about 584 (94)

Validation and Implementation of a Diagnostic Algorithm for DNA Detection of Bordetella pertussis, B. parapertussis, and B. holmesii in a Pediatric Referral Hospital in Barcelona, Spain [PDF]

Journal of Clinical Microbiology, 2019
This study aimed to validate a comprehensive diagnostic protocol based on real-time PCR for the rapid detection and identification of Bordetella pertussis , Bordetella parapertussis , and Bordetella holmesii , as well as its implementation in the diagnostic routine of ...
Ana Valero-Rello, Desirée Henares, Père Godoy   +2 more
exaly   +10 more sources

A Case Report of Bordetella holmesii Concomitant Vascular Graft Infection and Native Vertebral Osteomyelitis [PDF]

Case Reports in Infectious Diseases
Bordetella holmesii is a fastidious Gram-negative coccobacillus that was first isolated in 1983. It is an infrequent human pathogen, predominantly limited to asplenic and other immunocompromised hosts.
Joya-Rita Hindy, Ryan W. Stevens, Sergio L. Alvarez-Mulett, Aaron J. Tande, Walter R. Wilson, Aditya S. Shah   +5 more
doaj   +2 more sources

Emergence of Bordetella holmesii–Associated Pertussis-Like Illness, Northern India, 2019–2023 [PDF]

Emerging Infectious Diseases
We investigated Bordetella holmesii and Bordetella pertussis in 935 suspected pertussis cases in northern India (2019–2023) using PCR and serology. B. holmesii showed increased prevalence in pertussis cases, particularly in older children, highlighting ...
Nishant Shekhar, Deepa Sharma, Surajit Chakraborty, Rajesh Kumar, Rajneesh Singh Rawat, Nirmal Kaundal, Pawan Kumar, Tigran Avagyan, Deeksha Chauhan, Neha Jain, Megha Sharma, Arun Kumar, Vikas Gautam   +12 more
doaj   +2 more sources

Looking beyond Pertussis in Prolonged Cough Illness of Young Children [PDF]

Vaccines, 2022
Pertussis, commonly known as whooping cough, is one of the most poorly controlled vaccine-preventable diseases in the world. South-East Asia is estimated to contribute the most to childhood disease burden while this remains largely unexplored in India ...
Rajlakshmi Viswanathan, Sanjay Bafna, Manohar Lal Choudhary, Monika Reddy, Savita Katendra, Shradha Maheshwari, Sheetal Jadhav   +6 more
doaj   +2 more sources

Comparison of Bordetella species identification among differing rt-PCR assays in the United States [PDF]

Microbiology Spectrum
In the United States, the general laboratory method for diagnosing pertussis, caused by Bordetella pertussis, is real-time PCR (rt-PCR) targeting insertion sequence 481 (IS481).
Matthew Cole, Ashley K. Simon, Amanda Faulkner, Tami Skoff, Maria L. Tondella, Clemente Montero, Melinda B. Nye, Margaret Williams   +7 more
doaj   +2 more sources

Do not let perfect be the enemy of good: the current reality of Bordetella testing [PDF]

Microbiology Spectrum
Diagnosis of Bordetella pertussis is made by using polymerase chain reaction (PCR) to detect insertion sequence 481 (IS481). However, IS481 is found in both B. pertussis and Bordetella holmesii. In a recent study, Cole et al.
Rebecca Yee
doaj   +2 more sources

Positivity analysis of Bordetella spp. and SARS-CoV-2 coinfections in clinical samples from Colombia, 2021–2022 [PDF]

Microbiology Spectrum
Pertussis and COVID-19 are respiratory diseases with similar clinical manifestations, complicating diagnosis. In Colombia, pertussis surveillance is based on probable cases confirmed through laboratory detection of Bordetella pertussis.
Valentina Bonilla-Bravo, Efrain Montilla-Escudero, Ximena Castro Martinez, Fabiola Rojas, Carolina Duarte, Sandra Aparicio, Sergio Yebrail Gómez-Rangel, Paula Rodriguez Romero, Sandra Lucero Bonilla, Franklyn Prieto Alvarado, Jaid Rojas Sotelo, Gloria Rey Benito   +11 more
doaj   +2 more sources

Genome-based prediction of cross-protective, HLA-DR-presented epitopes as putative vaccine antigens for multiple Bordetella species [PDF]

Microbiology Spectrum
Acellular pertussis vaccines protect against severe pertussis, but vaccine-induced immunity wanes over time. Prior animal studies showed that T-cell responses are integral to long-lasting immunity.
Muktha S. Natrajan, Jesse M. Hall, Michael R. Weigand, Yanhui Peng, Margaret M. Williams, Mohamed Momin, Frederick Heath Damron, Purnima Dubey, Maria Lucia Tondella, Lucia C. Pawloski   +9 more
doaj   +2 more sources

Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site. [PDF]

J Clin Microbiol, 2004
Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussis and Bordetella holmesii.
Vielemeyer O, Crouch JY, Edberg SC, Howe JG.   +3 more
europepmc   +5 more sources

Detection of Bordetella holmesii Using Bordetella pertussis IS 481 PCR Assay [PDF]

Journal of Clinical Microbiology, 2000
After our manuscript comparing the performance of PCR, culture, and direct fluorescent-antibody testing for Bordetella pertussis ([3][1]) went to press, we obtained six isolates of Bordetella holmesii for the purpose of testing with our B. pertussis IS 481 PCR assay.
M J, Loeffelholz, C J, Thompson, K S, Long, M J, Gilchrist   +3 more
openaire   +2 more sources