Validation and Implementation of a Diagnostic Algorithm for DNA Detection of Bordetella pertussis, B. parapertussis, and B. holmesii in a Pediatric Referral Hospital in Barcelona, Spain. [PDF]
J Clin Microbiol, 2019 This study aimed to validate a comprehensive diagnostic protocol based on real-time PCR for the rapid detection and identification of Bordetella pertussis , Bordetella parapertussis , and Bordetella holmesii , as well as its implementation in the diagnostic routine of ...
Valero-Rello A +6 more
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Evaluation of real-time PCR for diagnosis of
BMC Infectious Diseases, 2006 Background Nucleic acid amplification of the IS481 region by PCR is more sensitive than culture for detection and diagnosis of Bordetella pertussis but the assay has known cross-reactivity for Bordetella holmesii and its use as a routine diagnostic assay
Fox Julie D +3 more
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Emergence of Bordetella holmesii–Associated Pertussis-Like Illness, Northern India, 2019–2023 [PDF]
Emerging Infectious DiseasesWe investigated Bordetella holmesii and Bordetella pertussis in 935 suspected pertussis cases in northern India (2019–2023) using PCR and serology. B. holmesii showed increased prevalence in pertussis cases, particularly in older children, highlighting ...
Nishant Shekhar +12 more
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Looking beyond Pertussis in Prolonged Cough Illness of Young Children [PDF]
Vaccines, 2022 Pertussis, commonly known as whooping cough, is one of the most poorly controlled vaccine-preventable diseases in the world. South-East Asia is estimated to contribute the most to childhood disease burden while this remains largely unexplored in India ...
Rajlakshmi Viswanathan +6 more
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Comparison of Bordetella species identification among differing rt-PCR assays in the United States [PDF]
Microbiology SpectrumIn the United States, the general laboratory method for diagnosing pertussis, caused by Bordetella pertussis, is real-time PCR (rt-PCR) targeting insertion sequence 481 (IS481).
Matthew Cole +7 more
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Do not let perfect be the enemy of good: the current reality of Bordetella testing [PDF]
Microbiology SpectrumDiagnosis of Bordetella pertussis is made by using polymerase chain reaction (PCR) to detect insertion sequence 481 (IS481). However, IS481 is found in both B. pertussis and Bordetella holmesii. In a recent study, Cole et al.
Rebecca Yee
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Transmission of Bordetella holmesii during Pertussis Outbreak, Japan [PDF]
Emerging Infectious Diseases, 2012 We describe the epidemiology of a pertussis outbreak in Japan in 2010–2011 and Bordetella holmesii transmission. Six patients were infected; 4 patients were students and a teacher at the same junior high school.
Hajime Kamiya +14 more
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Identification of Bordetella holmesii among the patients hospitalized with suspicion of pertussis and pertussis-like illnesses [PDF]
Журнал инфектологии, 2019 Purpose. To reveal and estimate prevalence of B. holmesii among the patients hospitalized with suspicion pertussis and pertussis-like illnesses.Materials and methods.
N. T. Gadua +12 more
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Positivity analysis of Bordetella spp. and SARS-CoV-2 coinfections in clinical samples from Colombia, 2021–2022 [PDF]
Microbiology SpectrumPertussis and COVID-19 are respiratory diseases with similar clinical manifestations, complicating diagnosis. In Colombia, pertussis surveillance is based on probable cases confirmed through laboratory detection of Bordetella pertussis.
Valentina Bonilla-Bravo +11 more
doaj +2 more sources
Genome-based prediction of cross-protective, HLA-DR-presented epitopes as putative vaccine antigens for multiple Bordetella species [PDF]
Microbiology SpectrumAcellular pertussis vaccines protect against severe pertussis, but vaccine-induced immunity wanes over time. Prior animal studies showed that T-cell responses are integral to long-lasting immunity.
Muktha S. Natrajan +9 more
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