Results 91 to 100 of about 1,184,647 (254)
Adenosine triphosphate as a modulator of protein interactions and stability
FEBS Open Bio, EarlyView.ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Molecules, 2014 Phytophenols are considered to have beneficial effects towards human physiology. They are food microcomponents with potent chemopreventive properties towards the most three frequent contemporary human diseases, e.g., cardiovascular alterations, cancer ...
Norbert Latruffe +4 more
doaj +1 more source
THE SEROLOGICAL RELATIONSHIP OF BOVINE WHEY ALBUMIN TO SERUM ALBUMIN
Journal of Biological Chemistry, 1950 Serological reactions are becoming increasingly important in the development of quantitative methods for the determination and characterization of native proteins. These reactions provide a basis for analytical methods that combine high sensitivity with a high degree of specificity.
E J, COULSON, H, STEVENS
openaire +2 more sources
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Design of a bubble-swarm bioreactor for animal cell culture [PDF]
, 1994 Gudermann F, Lütkemeyer D, Lehmann J. Design of a bubble-swarm bioreactor for animal cell culture. Cytotechnology. 1994;15(1-3):301-309.A stationary bubble-swarm has been used to aerate a mammalian cell culture bioreactor with an extremely low gas flow ...
Lehmann, Jürgen +2 more
core +1 more source
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective Facioscapulohumeral muscular dystrophy (FSHD) is one of the most debilitating and common muscular dystrophies. Despite its severity, no approved therapy exists for FSHD patients. However, several therapeutic candidates are currently under development, and some have recently entered clinical trials, marking the need for reliable ...
Mustafa Bilal Bayazit +11 more
wiley +1 more source
SHEAR FLOW OF BOVINE SERUM ALBUMIN SOLUTIONS
Studia Universitatis Babes-Bolyai Chemia, 2019 In this paper, the flow of bovine serum albumin (BSA) solutions in physiological serum was investigated at different concentrations from 1 to 10 g/dL and temperatures from 4°C to 80°C.
Maria BERCEA +3 more
doaj +1 more source
Synthetic nanoparticles of bovine serum albumin with entrapped salicylic acid
, 2016 ES Bronze-Uhle,1 BC Costa,1 VF Ximenes,2 PN Lisboa-Filho1 1Department of Physics, São Paulo State University (Unesp), School of Sciences, Bauru, São Paulo, Brazil; 2Department of Chemistry, São Paulo State University (Unesp), School ...
Ximenes VF +3 more
core
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Background Hereditary Spastic Paraplegia (HSP) comprises a group of rare genetic diseases characterized by length‐dependent axonal degeneration of the corticospinal tracts and dorsal columns, whose main clinical feature is spastic gait. Pathogenic variants in the SPG4 gene cause Spastic Paraplegia Type 4 (SPG4‐HSP), the most common form of HSP.
Gaia Fattorini +12 more
wiley +1 more source