Results 61 to 70 of about 721,610 (311)

Translational and rotational motions of albumin sensed by a non-covalent associated porphyrin under physiological and acidic conditions: a fluorescence correlation spectroscopy and time resolved anisotropy study. [PDF]

, 2008
The interaction between a free-base, anionic water-soluble porphyrin, TSPP, and the drug carrier protein, bovine serum albumin (BSA) has been studied by time-resolved fluorescence anisotropy (TRFA) and fluorescence correlation spectroscopy (FCS) at two ...
Andrade, S.M., Borst, J.W., Costa, S.M., Hoek, A., van, Visser, A.J.W.G.   +4 more
core   +2 more sources

CDK11 inhibition induces cytoplasmic p21WAF1 splice variant by p53 stabilisation and SF3B1 inactivation

Molecular Oncology, EarlyView.
CDK11 inhibition stabilises the tumour suppressor p53 and triggers the production of an alternative p21WAF1 splice variant p21L, through the inactivation of the spliceosomal protein SF3B1. Unlike the canonical p21WAF1 protein, p21L is localised in the cytoplasm and has reduced cell cycle‐blocking activity.
Radovan Krejcir, Lukasz Arcimowicz, Lucia Martinkova, Vaclav Hrabal, Filip Zavadil Kokas, Tomas Henek, Martina Kucerikova, Ondrej Bonczek, Pavlina Zatloukalova, Lenka Hernychova, Philip J. Coates, Borivoj Vojtesek, David P. Lane   +12 more
wiley   +1 more source

Terminal deoxynucleotidyltransferase. Serological studies and radioimmunoassay [PDF]

, 1976
Mouse antisera against calf terminal deoxynucleotidyltransferase (terminal transferase) have been prepared. The sera have been used to characterize terminal transferase both by studying inhibition of enzyme activity and by developing a competition ...
Baltimore, David, Gottlieb, Paul D., Kung, Patrick C.   +2 more
core  

Cytoplasmic p21 promotes stemness of colon cancer cells via activation of the NFκB pathway

Molecular Oncology, EarlyView.
Cytoplasmic p21 promotes colorectal cancer stem cell (CSC) features by destabilizing the NFκB–IκB complex, activating NFκB signaling, and upregulating BCL‐xL and COX2. In contrast to nuclear p21, cytoplasmic p21 enhances spheroid formation and stemness transcription factor CD133.
Arnatchai Maiuthed, Kerstin Huebner, Katharina Erlenbach‐Wuensch, Chuanpit Hampel, Daniela Thalheim, Adriana Vial‐Roehe, Bodee Nutho, Susanne Merkel, Arndt Hartmann, Pithi Chanvorachote, Regine Schneider‐Stock   +10 more
wiley   +1 more source

Milk whey protein concentration and mRNA associated with β-lactoglobulin phenotype [PDF]

, 2000
Two common genetic variants of β-lactoglobulin (β-lg), A and B, exist as co- dominant alleles in dairy cattle (Aschaffenburg, 1968). Numerous studies have shown that cows homozygous for β-lg A have more β-lg and less α-lactalbumin (α-la) and casein in ...
Auldist, Martin J., L'Huillier, Phil J., Langley, Brett, McLaren, Robert D., Molan, Peter C., Prosser, Colin G., Turner, Sally-Anne   +6 more
core   +2 more sources

Three method-combination protocol for improving purity of extracellular vesicles [PDF]

, 2020
Extracellular vesicles (EVs) are nanosized structures able to carry proteins, lipids and genetic material from one cell to another with critical implications in intercellular communication mechanisms.
Giamas, Georgios, Kumaran, Anish, Simon, Thomas, Veselu, Diana-Florentina   +3 more
core   +1 more source

ATF4‐mediated stress response as a therapeutic vulnerability in chordoma

Molecular Oncology, EarlyView.
We screened 5 chordoma cell lines against 100+ inhibitors of epigenetic and metabolic pathways and kinases and identified halofuginone, a tRNA synthetase inhibitor. Mechanistically halofuginone induces an integrated stress response, with eIF2alpha phosphorylation, activation of ATF4 and its target genes CHOP, ASNS, INHBE leading to cell death ...
Lucia Cottone, James Dunford, Eleanor Calcutt, Vicki Gamble, Filiz Senbabaoglu Aksu, Lorena Ligammari, Georgina Wherry, Giorgia Gaeta, John C. Christianson, Adrienne M. Flanagan, Udo Oppermann, Adam P. Cribbs   +11 more
wiley   +1 more source

Diffusion‐based size determination of solute particles: a method adapted for postsynaptic proteins

FEBS Open Bio, EarlyView.
We present a diffusion‐based approach for measuring the size of macromolecules and their complexes, and demonstrate its use on postsynaptic proteins. The method requires fluorescein‐labelled protein samples, a microfluidic device that maintains laminar flow for said samples, a microscope recording the emitted fluorescent signals, and an analytic ...
András László Szabó, Eszter Nagy‐Kanta, Soma Varga, Edit Andrea Jáger, Csaba István Pongor, Mária Laki, András József Laki, Zoltán Gáspári   +7 more
wiley   +1 more source

Serum-free cultured meat production by using Pichia pastoris-derived recombinant albumin

iScience
Summary: Cultured meat production involves the growth, differentiation, and food processing of muscle satellite cells. However, its heavy reliance on fetal bovine serum (FBS) significantly increases production costs, which has prompted interest in ...
Ye Rim Kim, Doyoung Hwang, Si Won Jang, Jae Ho Han, Ga Rim Na, Ji Hoon Park, Gyeongho Tae, Seung Moon Park, Hyun Woo Choi   +8 more
doaj   +1 more source

Human serum albumin nanoparticles loaded with phthalocyanine dyes for potential use in photodynamic therapy of atherosclerotic plaques [PDF]

, 2019
Diseases caused by obstruction or rupture of vulnerable plaques in the arterial walls such as cardiovascular infarction or stroke are the leading cause of death in the world.
Aljarilla, Ana, Banerjee, Shubhadeep, Escosura, Andres de la, Holappa, Lari, Krol, Silke, Martinelli, Chiara, Mäkinen, Petri I., Sengupta, Jayeeta, Setaro, Francesca, Szebeni, Janos, Torres, Tomas, Trohopoulos, Panagiotis N., Urbanics, Rudolf, Wu, LinPing, Ylä-Herttuala, Seppo   +14 more
core   +1 more source