Results 61 to 70 of about 2,154,775 (256)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Ninth Flyer 3_3_1993

, 2014
The quarterly newsletter, The Ninth Flyer, published by the Ninth Air Force Association, Inc.
9th Air Force Association
core   +1 more source

The analysis of different partial wear degrees of cutter on the rock-breaking effect

KSCE Journal of Civil Engineering
When the tunnel boring machine (TBM) is engaged in rock-breaking operations, the cutters are subject to wear due to the harsh underground environment.
Peng Zhou, Jianxu Shang, Zinan Wang, Jiacan Xu, Haoxuan Zhao   +4 more
doaj   +1 more source

Draft task force report

, 2014
This archived document is maintained by the Oregon State Library as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Title from PDF cover (viewed on November 19, 2014)"Draft for

core  

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Ninth Flyer 18_2_2008

, 2014
The quarterly newsletter, The Ninth Flyer, published by the Ninth Air Force Association, Inc.
9th Air Force Association
core  

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Rock Breaking Mechanism and Experimental Study of Axe-shaped PDC Cutter

Shiyou jixie, 2022
Axe-shaped PDC cutter has more obvious rock breaking advantage than conventional PDC cutter. In order to analyze the rock breaking mechanism of the axe-shaped PDC cutter, the finite element simulation and test were used to conduct comparison and force ...
Zou Deyong, Pan Long, Cui Yudong, Xu Jianfei   +3 more
doaj  

Ninth Flyer 18_1_2008

, 2014
The quarterly newsletter, The Ninth Flyer, published by the Ninth Air Force Association, Inc.
9th Air Force Association
core  

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source