Results 41 to 50 of about 25,994 (307)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A General Copper-Catalyzed Coupling of Azoles with Vinyl Bromides
, 2016 A copper-catalyzed methodology for the coupling of vinyl bromides with azoles has been developed. This protocol uses a combination of 10 mol % of copper iodide and 20 mol % of ethylenediamine as catalyst.
Qian Liao (1489453) +3 more
core +1 more source
Facile synthesis of functionalized spiro[indoline-3,2'-oxiran]-2-ones by Darzens reaction
Beilstein Journal of Organic Chemistry, 2013 A series of functionalized spiro[indoline-3,2'-oxiran]-2-ones was efficiently synthesized by Darzens reaction of phenacyl bromides with isatins both with N-alkyl groups and without N-substituent in the presence of potassium carbonate as a base catalyst ...
Qin Fu, Chao-Guo Yan
doaj +1 more source
FEBS Open Bio, EarlyView.NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks +4 more
wiley +1 more source
Copper-Catalyzed Trifluoromethylation of Alkyl Bromides
, 2019 Copper oxidative addition into organohalides is a challenging two-electron process. In contrast, formal oxidative addition of copper to Csp2 carbon–bromine bonds can be accomplished by employing latent silyl radicals under photoredox conditions.
David W. C. MacMillan (1314639) +3 more
core +1 more source
Molecules, 2014 An efficient and general copper-catalyzed Goldberg reaction at 90–110 °C between aryl bromides and amides providing the desired products in good to excellent yields has been developed using N,N-dimethylglycine as the ligand.
Liqin Jiang
doaj +1 more source
Nature Communications, 2022 Synthetic methodologies that enable the formation of C‒Si bonds in organic molecules are desirable. Here, the authors report the nickel-catalyzed reductive alkylsilylation of acrylonitrile with unactivated alkyl bromides and chlorosilanes.
Jinwei Sun +5 more
doaj +1 more source
FEBS Open Bio, EarlyView.TGF‐β1 stimulation downregulates lysosomal channel TRPML1 in pulmonary fibroblasts. The TRPML1 agonist ML‐SA5 suppressed fibroblast‐to‐myofibroblast activation and collagen production. Mechanistically, ML‐SA5 inhibited mTOR phosphorylation, restored autophagic flux, and its effects were enhanced by rapamycin (mTOR inhibitor) and reversed by MHY1485 ...
Jiatong Yao +10 more
wiley +1 more source
Reductive Cross-Coupling of Nonaromatic, Heterocyclic Bromides with Aryl and Heteroaryl Bromides
, 2016 Reductive cross-coupling allows the direct C–C bond formation between two organic halides without the need for preformation of an organometallic reagent.
Gary A. Molander (1314150) +2 more
core +1 more source
Molecules, 2010 A practical synthetic route for the preparation of 2-pyridyl and 3-pyridyl derivatives has been accomplished by utilizing a simple coupling reaction of stable 2-pyridylzinc bromides and 3-pyridylzinc bromides.
Reuben D. Rieke, Seung-Hoi Kim
doaj +1 more source