Transposon Tn5 mutagenesis of Brucella abortus [PDF]
We demonstrate that transposon-mediated mutagenesis can be used to construct mutations in the pathogen Brucella abortus. We have used both a plasmid and a bacteriophage to introduce either Tn5 or Tn5 lac into the Brucella chromosome. B. abortus is naturally sensitive to kanamycin.
L D, Smith, F, Heffron
openaire +2 more sources
COMPARISON OF SEROLOGICAL INTERFERENCE FOLLOWING VACCINATION WITH BRUCELLA AB~RTUS STRAINS I N ROUTINE DIAGNOSTIC TESTS [PDF]
The serological interference following Brucella aborfus SR851 and S99 vaccination, in routine diagnostic tests was evaluafed in catf/e of four months and above. Sero negative animals were grouped into three.
K. N. Nimisha +4 more
doaj
Infecção em cão por Brucella abortus: relato de caso Brucella abortus infection in dog: case report
Brucella abortus infection is reported in a dog from a rural area that presented at clinical evaluation left testicular enlargement and right testicular decrease.
J. Megid +5 more
doaj +1 more source
Structural Insights into the HWE Histidine Kinase Family: The Brucella Blue Light-Activated Histidine Kinase Domain [PDF]
In response to light, as part of a two-component system, the Brucella blue light-activated histidine kinase (LOV-HK) increases its autophosphorylation, modulating the virulence of this microorganism.
Arrar, Mehrnoosh +9 more
core +1 more source
Significant Impact of Schmallenberg Virus in Three Ruminant Farms: A Laboratory Experience
Schmallenberg virus (SBV) is endemic in Europe and other parts of the world and represents an underestimated and underdiagnosed cause of abortion and economic losses for livestock farms. In the present study, we evaluated SBV’s impact on three ruminant farms, in particular, two dairy cattle farms with reproductive problems (including abortions ...
Jacopo Guccione +5 more
wiley +1 more source
Highly Sensitive Bacteriophage-Based Detection of Brucella abortus in Mixed Culture and Spiked Blood
For decades, bacteriophages (phages) have been used for Brucella species identification in the diagnosis and epidemiology of brucellosis. Traditional Brucella phage typing is a multi-day procedure including the isolation of a pure culture, a step that ...
Kirill V. Sergueev +2 more
doaj +1 more source
Phylogenetic analysis reveals an ancient gene duplication as the origin of the MdtABC efflux pump. [PDF]
The efflux pumps from the Resistance-Nodulation-Division family, RND, are main contributors to intrinsic antibiotic resistance in Gram-negative bacteria. Among this family, the MdtABC pump is unusual by having two inner membrane components.
Górecki, Kamil, McEvoy, Megan M
core +2 more sources
Abstract In the coming decades, there is expected to be a sharply increased demand for dietary proteins for humans and animals. As a result, there is an increasing focus on reared insects as a new source of protein. According to the Ministry of Agriculture, Nature and Food Quality (LNV), the use of food chain residual flows such as former foodstuffs as
L.F.F. Kox, D.T.H.M. Sijm
wiley +1 more source
The small protein CydX is required for function of cytochrome bd oxidase in Brucella abortus. [PDF]
A large number of hypothetical genes potentially encoding small proteins of unknown function are annotated in the Brucella abortus genome. Individual deletion of 30 of these genes identified four mutants, in BAB1_0355, BAB2_0726, BAB2_0470, and BAB2_0450
de Jong, Maarten F +5 more
core +3 more sources
BLOC1S1 Attenuates B. Melitensis 16M LPS‐Triggered Autophagy by Spatial Confinement of TDP‐43
This study explores how B. melitensis 16M LPS and BLOC1S1 regulate autophagy in goat spermatogonial stem cells. The 16M LPS is activating AMPK‐dependent autophagy, increasing LC3B‐II/I ratios and lysosomal genes, while Escherichia coli LPS shows no effect.
Shicheng Wan +13 more
wiley +1 more source

