Bulked-segregant analysis by deep sequencing (BSA-seq) is a widely used method for mapping QTL (quantitative trait loci) due to its simplicity, speed, cost-effectiveness, and efficiency.
Weiren Wu, Lihui Lin, Xiaofang Xie
exaly +3 more sources
Identification of QTLs and new candidate genes affecting ear shank length via BSA-seq and transcriptomic analysis in maize [PDF]
IntroductionThe ear shank, a short branch connecting the stalk and the ear, represented a key agronomic trait influenced both yield and plant architecture in maize, yet the molecular mechanism remained not fully understood.MethodsIn this study, BSA-seq ...
Hongzhou An +6 more
doaj +2 more sources
Identification of candidate genes controlling chilling tolerance of rice in the cold region at the booting stage by BSA-Seq and RNA-Seq [PDF]
Rice is sensitive to low temperatures, specifically at the booting stage. Chilling tolerance of rice is a quantitative trait loci that is governed by multiple genes, and thus, its precise identification through the conventional methods is an arduous task.
Pan Guojun, Guo Zhenhua, Cai Lijun
exaly +2 more sources
Identification of Dwarfing Candidate Genes in Brassica napus L. LSW2018 through BSA–Seq and Genetic Mapping [PDF]
Plant height, as a crucial component of plant architecture, exerts a significant influence on rapeseed (Brassica napus L.) lodging resistance, photosynthetic efficiency, yield, and mechanized harvest level.
Sha Huang +6 more
doaj +2 more sources
Combined BSA-seq and RNA-seq approaches reveal candidate genes associated with seed weight in Brassica napus [PDF]
Brassica napus, a globally significant oilseed crop of the Brassicaceae family, serves as a major source of vegetable oil and biofuel. Seed size/weight is a crucial agronomic trait that directly determines crop yield.
Xinxin Geng +26 more
doaj +2 more sources
As a Brassica crop, Brassica napus typically has single flowers that contain four petals. The double-flower phenotype of rapeseed has been a desirable trait in China because of its potential commercial value in ornamental tourism.
Xiaowei Ma +12 more
doaj +2 more sources
Revealing the key role of the folate synthesis regulatory gene DHNA in tobacco leaf yellowing based on BSA-seq, RNA-seq, and proteomic sequencing [PDF]
Background The degree of yellowing in tobacco leaves is an important indicator for determining the maturity and harvesting time of tobacco leaves. Decreasing chlorophyll levels helps speed up the ripening process of tobacco leaves for easier mechanical ...
Qiuhe Pan +6 more
doaj +2 more sources
Mapping of a QTL associated with sucrose content in peanut kernels using BSA-seq. [PDF]
As an important factor affecting the edible quality of peanut kernels, sucrose content is a complex quantitative trait regulated by multiple factors. In this study, an F2 segregating population and a recombinant inbred line (RIL) population, derived from a cross between the high sucrose content variety Jihuatian 1 and the low sucrose content line ...
Guo J +19 more
europepmc +4 more sources
Identification of Potential Candidate Dwarf Genes in Maize by BSA-Seq and Transcriptomic Analyses. [PDF]
Maize (Zea mays L.) is a globally important crop. Understanding plant height and the underlying dwarfing genes is of critical importance for improving lodging resistance and optimizing plant architecture. In this study, a dwarf mutant designated CM17-2 was obtained by exposing the maize inbred line B73 to carbon ion beam irradiation.
Zhang L +7 more
europepmc +2 more sources
Most plant traits are governed by polygenes including both major and minor genes. Linkage mapping and positional cloning have contributed greatly to mapping genomic loci controlling important traits in crop species. However, they are low-throughput, time-
Aasim Majeed +6 more
doaj +1 more source

