Results 71 to 80 of about 6,432 (211)

Design and optimisation of rapid real‐time PCR assays for the detection of key Culicoides species

Medical and Veterinary Entomology, EarlyView.
Species‐specific real‐time PCR assays were designed and optimised using primer/probe sets targeting C. obsoletus, C. scoticus, C. chiopterus, C. dewulfi, C. pulicaris, C. punctatus and C. impunctatus. Primer specificity and amplification efficiency were validated through in silico specificity analysis and real‐time PCR amplification profiling.
Elsie Isiye, Angela Valcarcel Olmeda, Thomas Curran, David O'Neill, Theo De Waal, Gerald Barry, Aidan O'Hanlon, James O'Shaughnessy, Marion England, Annetta Zintl, Denise O'Meara   +10 more
wiley   +1 more source

Western blot analysis of BTV-8 in KC cells. [PDF]

, 2013
BTV-8 infected KC cell lysates analysed by SDS PAGE/Western blot using anti-BTV NS4 antibodies. BTVKC7 = KC cells harvested at 7 days post-infection. M indicates the molecular weight marker, labelled in kDa.
Mourad Belhouchet (321963), Jonathan M. Grimes (191832), Peter P. C. Mertens (177259), Fauziah Mohd Jaafar (321960), Houssam Attoui (177251), Andrew E. Firth (122998)   +5 more
core   +1 more source

Full-Genome Sequencing as a Basis for Molecular Epidemiology Studies of Bluetongue Virus in India.

PLoS ONE, 2015
Since 1998 there have been significant changes in the global distribution of bluetongue virus (BTV). Ten previously exotic BTV serotypes have been detected in Europe, causing severe disease outbreaks in naïve ruminant populations.
Sushila Maan, Narender S Maan, Manjunatha N Belaganahalli, Pavuluri Panduranga Rao, Karam Pal Singh, Divakar Hemadri, Kalyani Putty, Aman Kumar, Kanisht Batra, Yadlapati Krishnajyothi, Bharat S Chandel, G Hanmanth Reddy, Kyriaki Nomikou, Yella Narasimha Reddy, Houssam Attoui, Nagendra R Hegde, Peter P C Mertens   +16 more
doaj   +1 more source

Vaccination as a Strategy to Prevent Bluetongue Virus Vertical Transmission

Pathogens, 2021
Bluetongue virus (BTV) produces an economically important disease in ruminants of compulsory notification to the OIE. BTV is typically transmitted by the bite of Culicoides spp., however, some BTV strains can be transmitted vertically, and this is ...
José M. Rojas, Verónica Martín, Noemí Sevilla   +2 more
doaj   +1 more source

Maturation of spinal motor control through developmental changes in ion currents

The Journal of Physiology, EarlyView.
Abstract figure legend Ion channels of spinal neurons have been shown to change during development in a wide range of species. These changes occur at specific stages of development. An important developmental process is the maturation of motor control by which the body acquires new movements or refines movements already present.
Stephanie F. Gaudreau, Tuan V. Bui
wiley   +1 more source

The genome-segment composition of reassortants generated between BTV-1 [RSArrrr/01] and BTV-26 [KUW2010/02]. [PDF]

, 2016
The genome-segment composition of reassortants generated between BTV-1 [RSArrrr/01] and BTV-26 [KUW2010/02].
Gillian D. Pullinger (404039), Mark Boyce (510634), Peter P. Mertens (2150410), Marc Guimerà Busquets (2150407), Kyriaki Nomikou (177223), Houssam Attoui (177251)   +5 more
core   +1 more source

Vector Competence of Italian Populations of Culicoides for Some Bluetongue Virus Strains Responsible for Recent Northern African and European Outbreaks

Viruses, 2019
The distribution of Bluetongue virus (BTV) in Europe can be represented by two distinct and interconnected epidemiological systems (episystems), each characterized by different ecological characteristics and vector species.
Valentina Federici, Maria Goffredo, Giuseppe Mancini, Michela Quaglia, Adriana Santilli, Francesca Di Nicola, Matteo De Ascentis, Pierangela Cabras, Carmela Volpicelli, Claudio De Liberato, Giuseppe Satta, Giovanni Federico, Alessandra Leone, Maura Pisciella, Ottavio Portanti, Federica Pizzurro, Liana Teodori, Giovanni Savini   +17 more
doaj   +1 more source

Concurrent Temperature and Light Intensity Fluctuations Promote Stomatal Opening and Non‐Steady State Photosynthesis

Plant, Cell &Environment, Volume 49, Issue 8, Page 5005-5021, August 2026.
ABSTRACT In nature, fluctuations in light intensity (FL) are tightly coupled to rapid changes in leaf temperature (Tleaf), yet the short‐term physiological effects of these concurrent drivers remain largely unresolved. Here, we combined rapid infrared induced Tleaf fluctuations with controlled step changes in light intensity for leaf gas exchange ...
Samikshya Shrestha, Elias Kaiser, Sarah R. Berman, Maarten L. J. Wassenaar, Tom van den Berg, Evi Mavrou, Leo F. M. Marcelis, Silvere Vialet‐Chabrand   +7 more
wiley   +1 more source

Co-expression of the VP2, VP7 and NS1 BTV proteins. [PDF]

, 2013
(A) Immunofluorescence microscopy using a mouse polyclonal antibody against BTV-4 in BHK-21 cells transfected with pcDNA3-VP2, pcDNA3-VP7, pcDNA3-NS1 and different combinations of these plasmids. (B) Immunofluorescence microscopy using a mouse polyclonal
Javier Ortego (15045), Juan Anguita (55227), Eva Calvo-Pinilla (326514), Nicolás Navasa (326515)   +3 more
core   +1 more source

Secreted Nonstructural Protein 3 is a Pathogenic Determinant of Orbivirus

Advanced Science, Volume 13, Issue 42, 28 July 2026.
This study uncovers a conserved PIP2‐dependent secretory pathway of orbivirus NS3 that induces vascular leakage. Pharmacological disruption of PIP2‐NS3 interaction significantly reduces viral pathogenicity and provides protective efficacy in murine models, establishing PIP2‐mediated NS3 secretion as both a key virulence determinant and a promising ...
Junyong Guan, Dong Zhou, Ran Shao, Xing Liu, Jin Peng, Yingran Huang, Shuhui Qi, Cankun Xi, Menghang Wang, Bin Sun, Yinglin Qi, Xin Yin   +11 more
wiley   +1 more source