Results 11 to 20 of about 352,185 (244)

Loss of function of the Cik1/Kar3 motor complex results in chromosomes with syntelic attachment that are sensed by the tension checkpoint. [PDF]

PLoS Genetics, 2012
The attachment of sister kinetochores by microtubules emanating from opposite spindle poles establishes chromosome bipolar attachment, which generates tension on chromosomes and is essential for sister-chromatid segregation.
Fengzhi Jin, Hong Liu, Ping Li, Hong-Guo Yu, Yanchang Wang   +4 more
doaj   +1 more source

Extensive 5′-surveillance guards against non-canonical NAD-caps of nuclear mRNAs in yeast

Nature Communications, 2020
NAD (nicotinamide adenine dinucleotide) acts as a non-canonical RNA cap structure in bacteria and eukaryotes. Here the authors demonstrate the whole landscape of budding yeast NAD-RNAs which are subject to diverse surveillance pathways, suggesting that ...
Yaqing Zhang, David Kuster, Tobias Schmidt, Daniel Kirrmaier, Gabriele Nübel, David Ibberson, Vladimir Benes, Hans Hombauer, Michael Knop, Andres Jäschke   +9 more
doaj   +1 more source

GC-rich DNA elements enable replication origin activity in the methylotrophic yeast Pichia pastoris. [PDF]

PLoS Genetics, 2014
The well-studied DNA replication origins of the model budding and fission yeasts are A/T-rich elements. However, unlike their yeast counterparts, both plant and metazoan origins are G/C-rich and are associated with transcription start sites. Here we show
Ivan Liachko, Rachel A Youngblood, Kyle Tsui, Kerry L Bubb, Christine Queitsch, M K Raghuraman, Corey Nislow, Bonita J Brewer, Maitreya J Dunham   +8 more
doaj   +1 more source

Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ

eLife, 2023
Nuclear processes depend on the organization of chromatin, whose basic units are cylinder-shaped complexes called nucleosomes. A subset of mammalian nucleosomes in situ (inside cells) resembles the canonical structure determined in vitro 25 years ago ...
Zhi Yang Tan, Shujun Cai, Alex J Noble, Jon K Chen, Jian Shi, Lu Gan   +5 more
doaj   +1 more source

Single-molecule study reveals Hmo1, not Hho1, promotes chromatin assembly in budding yeast

mBio, 2023
Linker histone H1 plays a crucial role in various biological processes, including nucleosome stabilization, high-order chromatin structure organization, gene expression, and epigenetic regulation in eukaryotic cells.
Mengxue Wang, Jinghua Li, Yong Wang, Hang Fu, Haoning Qiu, Yanying Li, Ming Li, Ying Lu, Yu Vincent Fu   +8 more
doaj   +1 more source

Development of AlissAID system targeting GFP or mCherry fusion protein.

PLoS Genetics, 2023
Conditional control of target proteins using the auxin-inducible degron (AID) system provides a powerful tool for investigating protein function in eukaryotes. Here, we established an Affinity-linker based super-sensitive auxin-inducible degron (AlissAID)
Yoshitaka Ogawa, Kohei Nishimura, Keisuke Obara, Takumi Kamura   +3 more
doaj   +1 more source

Reliable budding pattern classification of yeast cells with time-resolved measurement of metabolite production

BioTechniques, 2022
Filamentous growth in Saccharomyces cerevisiae is a stress response commonly induced under nutrient deprivation and by certain alcohols. It is a compound phenotype characterized by pseudohyphal growth, invasion and a shift to more polarized budding ...
Michela Winters, Violetta Aru, Kate Howell, Nils Arneborg   +3 more
doaj   +1 more source

How to halve ploidy : lessons from budding yeast meiosis [PDF]

, 2012
Maintenance of ploidy in sexually reproducing organisms requires a specialized form of cell division called meiosis that generates genetically diverse haploid gametes from diploid germ cells.
Kerr, Gary W., Kerr, Gary, Sarkar, Sourav, Arumugam, Prakash   +3 more
core   +1 more source

The rim15-Endosulfine-PP2ACdc55 signalling module regulates entry into gametogenesis and quiescence via distinct mechanisms in budding yeast [PDF]

, 2013
Quiescence and gametogenesis represent two distinct survival strategies in response to nutrient starvation in budding yeast. Precisely how environmental signals are sensed by yeast cells to trigger quiescence and gametogenesis is not fully understood.
Sourav Sarkar, Dalgaard Jacob Z., Prakash Arumugam, Arumugam P., Sarkar, Sourav, Jonathan B. A. Millar, Sarkar S., Arumugam Prakash, Millar, Jonathan B. A., Jacob Z. Dalgaard (303312), Jacob Z. Dalgaard, Arumugam, Prakash, Sourav Sarkar (430861), Millar Jonathan B. A., Jonathan B. A. Millar (430863), Sarkar Sourav, Millar J.B.A., Jonathan B A Millar, Dalgaard, Jacob Z., Jacob Z Dalgaard, Dalgaard J.Z., Prakash Arumugam (303310)   +21 more
core   +1 more source

CDK-dependent nuclear localization of B-Cyclin Clb1 promotes FEAR activation during meiosis I in budding yeast [PDF]

, 2013
Cyclin-dependent kinases (CDK) are master regulators of the cell cycle in eukaryotes. CDK activity is regulated by the presence, post-translational modification and spatial localization of its regulatory subunit cyclin.
Sarkar, S. (Sourav), Sourav Sarkar, Novák, Béla, Tibbles, Katherine L., Prakash Arumugam, Arumugam P., Bela Novak (5648875), Sarkar S., Arumugam Prakash, Arumugam, Prakash, Sourav Sarkar (430861), Katherine Louise Tibbles (478963), Katherine Louise Tibbles, Bela Novak, Sarkar Sourav, Novak Bela, Tibbles Katherine Louise, Prakash Arumugam (303310), Tibbles K.L., Novak B.   +19 more
core   +2 more sources