Integration of Proteomics and Metabolomics Into the Design, Build, Test, Learn Cycle to Improve 3-Hydroxypropionic Acid Production in Aspergillus pseudoterreus [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 Biological engineering of microorganisms to produce value-added chemicals is a promising route to sustainable manufacturing. However, overproduction of metabolic intermediates at high titer, rate, and yield from inexpensive substrates is challenging in ...
Kyle R. Pomraning +21 more
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The human plasma lipidome response to exertional heat tolerance testing [PDF]
Lipids in Health and DiseaseBackground The year of 2023 displayed the highest average global temperatures since it has been recorded—the duration and severity of extreme heat are projected to increase. Rising global temperatures represent a major public health threat, especially to
Igor L. Estevao +14 more
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High-Throughput Large-Scale Targeted Proteomics Assays for Quantifying Pathway Proteins in Pseudomonas putida KT2440 [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 Targeted proteomics is a mass spectrometry-based protein quantification technique with high sensitivity, accuracy, and reproducibility. As a key component in the multi-omics toolbox of systems biology, targeted liquid chromatography-selected reaction ...
Yuqian Gao +40 more
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Further engineering of R. toruloides for the production of terpenes from lignocellulosic biomass [PDF]
Biotechnology for Biofuels, 2021 Background Mitigation of climate change requires that new routes for the production of fuels and chemicals be as oil-independent as possible. The microbial conversion of lignocellulosic feedstocks into terpene-based biofuels and bioproducts represents ...
James Kirby +21 more
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Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity [PDF]
mSphereCoronaviruses (CoV) emerge suddenly from animal reservoirs to cause novel diseases in new hosts. Discovered in 2012, the Middle East respiratory syndrome coronavirus (MERS-CoV) is endemic in camels in the Middle East and is continually causing local ...
Amy C. Sims +21 more
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Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolution [PDF]
Nature Communications, 2020 Imaging mass spectrometry is a powerful emerging tool for mapping the spatial distribution of biomolecules across tissue surfaces. Here the authors showcase an automated technology for deep proteome imaging that utilizes ultrasensitive microfluidics and ...
Paul D. Piehowski +13 more
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Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics [PDF]
Communications Biology, 2021 Tsai, Zhang, Scholten et al. develop a surfactant- assisted one-pot sample preparation coupled with mass spectrometry method (SOP-MS) for label-free global single-cell proteomics. This method allows researchers to measure hundreds of proteins from single
Chia-Feng Tsai +27 more
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Production of ent-kaurene from lignocellulosic hydrolysate in Rhodosporidium toruloides [PDF]
Microbial Cell Factories, 2020 Background Rhodosporidium toruloides has emerged as a promising host for the production of bioproducts from lignocellulose, in part due to its ability to grow on lignocellulosic feedstocks, tolerate growth inhibitors, and co-utilize sugars and lignin ...
Gina M. Geiselman +15 more
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Regulation of β-cell death by ADP-ribosylhydrolase ARH3 via lipid signaling in insulitis [PDF]
Cell Communication and SignalingBackground Lipids are regulators of insulitis and β-cell death in type 1 diabetes development, but the underlying mechanisms are poorly understood. Here, we investigated how the islet lipid composition and downstream signaling regulate β-cell death ...
Soumyadeep Sarkar +24 more
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A compendium of multi-omics data illuminating host responses to lethal human virus infections [PDF]
Scientific DataHuman infections caused by viral pathogens trigger a complex gamut of host responses that limit disease, resolve infection, generate immunity, and contribute to severe disease or death.
Amie J. Eisfeld +28 more
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