Results 211 to 220 of about 29,655,632 (304)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Phenoxazines with a Phototransferable <i>N</i>-Acetyl Group and Acrylate Linker: Assembly by C-H Activation, Photoconversion to Fluorescent Dyes, Biolabeling, and Super-Resolution Imaging. [PDF]

J Am Chem Soc
Savicheva E, Hubrich J, Khan TA, Bossi ML, Belov VN, Ackermann L, Hell SW.   +6 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Synthesis of Aza-indeno-aza-fluoranthene and Constitutional Isomers by Ring-Size Selective C-H Activation. [PDF]

Chemistry
Keck C, Rominger F, Garg S, Elstner M, Mastalerz M.   +4 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

From Research to an Undergraduate Lab Bench: An Undergraduate-Level Guided-Inquiry Experiment to Explore a Cp*Ir(III)-Catalyzed <i>ortho-</i>C-H Activation. [PDF]

J Chem Educ
Roque Peña J, Albaladejo-Flores CD, Alvarez-Zavala MG, De Jesús-Ortiz GY, Morales-Báez NM, Flores-Pérez G.   +5 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

A Change in C-H Activation Mechanism: Experimental and Computational Investigations of Rh-Catalyzed Disubstituted Benzene Functionalization. [PDF]

Organometallics
Reid CW, Zhang C, Baptiste LE, Houk KN, Goddard Iii WA, Gunnoe TB.   +5 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Versatile Butenolide Syntheses via a Structure-Oriented C-H Activation Reaction. [PDF]

J Am Chem Soc
Lin YK, Kim D, Ouyang Y, Yu JQ.
europepmc   +1 more source