Results 221 to 230 of about 29,655,632 (304)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Pd(II)-Catalyzed C-H Activation/<i>O</i>‑Annulation toward Succinimide-Embedded Tetracyclic Pyranonaphthoquinones and Their Application as Metal Sensors. [PDF]

ACS Omega
Kamaraj E, Kumar A, Mohandoss S, Thombal RS, Lim HN, Lee YR.   +5 more
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Directed C-H activation of 13α-estrone: a pathway to promising AKR1C inhibitors <i>via</i> docking and biological studies. [PDF]

RSC Adv
Mernyák E, Gjorgoška M, Sinreih M, Kotnik A, Zanoškar Z, Godec A, Jukič M, Bren U, Ignácz R, Szőri K, Kele Z, Hunyadi A, Rižner TL.   +12 more
europepmc   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Dynamic Hydrogen-Bonding Nanonetworks and Asymmetric Dual-Interface Built-In Electric Fields Cooperatively Mediate Proton-Coupled Electron Transfer for C─H Activation. [PDF]

Adv Sci (Weinh)
Han YW, Liu RY, Chen Y, Ye L, Gong TJ, Lu XB, Yan N, Fu Y.   +7 more
europepmc   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

<i>De novo</i> construction of C-O axial chirality <i>via</i> cobalt-catalyzed atroposelective C-H activation/annulation. [PDF]

Chem Sci
Zhang Y, Xie Y, Guo B, Dou Y, Yang D, Niu JL.   +5 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Selective Functionalization of 1-substituted-3-arylquinoxalin-2(1<i>H</i>)-ones <i>via</i> C-H activation. [PDF]

RSC Adv
Kumar P, Priya, Dutta S, Mishra D, Kumar N.   +4 more
europepmc   +1 more source