Results 51 to 60 of about 52,118 (265)

Deep Learning‐Driven Discovery and Engineering of an Efficient PETase for Depolymerization and Detoxification of PET Microplastics Under Physiological Conditions

Advanced Science, EarlyView.
A deep learning–driven pipeline mining 246 million protein sequences uncovers AhPETase, an evolutionarily distinct PET hydrolase. Engineered variant AhPETaseM1 degrades post‐consumer PET microplastics under physiological conditions and reverses microplasticinduced cytotoxicity in human lung and colon cells, establishing enzymatic microplastic ...
Yuxuan Wang, Shijie He, Yuheng Chang, Sheng Mao, Jianan Canal Li, Binbin Chen, Hongxun Gao, Mingchun Xu, Chenxu Liu, Yajie Wang   +9 more
wiley   +1 more source

Extracellular Signals of a Human Epithelial Colorectal Adenocarcinoma (Caco-2) Cell Line Facilitate the Penetration of Pseudomonas aeruginosa PAO1 Strain through the Mucin Layer

Frontiers in Cellular and Infection Microbiology, 2017
Pseudomonas aeruginosa can penetrate the layer of mucus formed by host intestinal epithelial cells, often resulting in sepsis in immunocompromised patients. We have previously demonstrated that P.
Naoki Hayashi, Atsushi Yokotani, Masami Yamamoto, Mariko Kososhi, Mayu Morita, Chiaki Fukunishi, Nagisa Nishizawa, Naomasa Gotoh   +7 more
doaj   +1 more source

Development of Caco-2 cells expressing four CYPs via a mammalian artificial chromosome

BMC Biotechnology, 2020
Background Oral administration is the most common way to deliver drugs to the systemic circulation or target organs. Orally administered drugs are absorbed in the intestine and metabolized in the intestine and liver.
Yumi Ohta, Kanako Kazuki, Satoshi Abe, Mitsuo Oshimura, Kaoru Kobayashi, Yasuhiro Kazuki   +5 more
doaj   +1 more source

Intestinal Cell Tight Junctions Limit Invasion of Candida albicans through Active Penetration and Endocytosis in the Early Stages of the Interaction of the Fungus with the Intestinal Barrier. [PDF]

PLoS ONE, 2016
C. albicans is a commensal yeast of the mucous membranes in healthy humans that can also cause disseminated candidiasis, mainly originating from the digestive tract, in vulnerable patients.
Marianne Goyer, Alicia Loiselet, Fabienne Bon, Coralie L'Ollivier, Michael Laue, Gudrun Holland, Alain Bonnin, Frederic Dalle   +7 more
doaj   +1 more source

Expression pattern of Dkk-3, a secreted Wnt pathway inhibitor, in mouse intestinal tissue and three-dimensional cultured Caco-2 spheroids

Journal of Stem Cells and Regenerative Medicine, 2015
We investigated the expression pattern of Dkk-3, a secreted Wnt pathway inhibitor, in mouse intestinal tissue and three-dimensional cultured Caco-2 spheroids. Dkk-3 was expressed at the bottom of crypts from the mouse small intestine.
Kataoka K, Rikitake Y, Ayabe Y
doaj  

Construction of Caco-2 cell hollow fiber bioreactor for the study on functional substances

浙江大学学报. 农业与生命科学版, 2016
Human colon carcinoma cell line Caco-2 has been extensively used over the last thirty years as a model of the intestinal barrier. The parental cell line undergoes a process of spontaneous differentiation that leads to the formation of a monolayer of ...
XU Dongdong, CHU Qiang, YANG Yunyun, LÜ Xiangmin, ZHANG Yanzhen, ZHENG Xiaodong   +5 more
doaj   +1 more source

Spectroscopic and In Silico Determination of the Structural and Antioxidant Properties of Essential Oils From Lavandula Species Cultivated in the Silivri Region of Istanbul

Journal of the American Oil Chemists' Society, EarlyView.
ABSTRACT Plants are important sources of bioactive compounds that contribute to nutrition, health maintenance, and disease prevention. In this study, the chemical composition and antioxidant potential of essential oils obtained from Lavandula angustifolia “Sevtopolis” and Lavandula × intermedia cultivated in the Silivri region of Istanbul were ...
Bilge Bicak, Beray Kizilkaya, Serda Kecel Gunduz, Aliye Demet Demirag, Sabriye Percin Ozkorucuklu   +4 more
wiley   +1 more source

Evaluation of the Expression of Virulence Factors of V. cholerae After Interaction With the Human Colon Adenocarcinoma (Caco-2) Cell Line

Canadian Journal of Infectious Diseases and Medical Microbiology
Cholera remains a global challenge, and understanding how V. cholerae adapts to environmental condition is essential for innovating new management strategies. This research aims to examine the expression of ctxAB, tcpA, and hlyA genes in V.
Mohammadreza Kheradmand, Masoumeh Saberpour, Bita Bakhshi, Mahboube Bahroudi   +3 more
doaj   +1 more source

Compartmentalized 3D Tissue Culture Arrays under Controlled Microfluidic Delivery

Scientific Reports, 2017
We demonstrate an in vitro microfluidic cell culture platform that consists of periodic 3D hydrogel compartments with controllable shapes. The microchip is composed of approximately 500 discontinuous collagen gel compartments locally patterned in between
Burcu Gumuscu, Hugo J. Albers, Albert van den Berg, Jan C. T. Eijkel, Andries D. van der Meer   +4 more
doaj   +1 more source

Modulation of Animal Gut Health by Milk Oligosaccharide

Animal Research and One Health, EarlyView.
This review summarizes the composition and structural characteristics of MOs across different mammalian species, and elucidates their roles and mechanisms in maintaining intestinal homeostasis by regulating gut microbiome and enhancing intestinal chemical, physical and immune barriers.
Ningtao Chen, Nana Jing, Shiyu Tao, Mingyue Fei, Wentao Lv, Hua Yang, Yingping Xiao, Haiqin Wu   +7 more
wiley   +1 more source