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In vivo Calcium Imaging of Evoked Calcium Waves in the Embryonic Cortex [PDF]
Frontiers in Cellular Neuroscience, 2016 The dynamics of intracellular calcium fluxes are instrumental in the proliferation, differentiation, and migration of neuronal cells. Knowledge thus far of the relationship between these calcium changes and physiological processes in the developing brain has derived principally from ex vivo and in vitro experiments.
Ville Jokinen +2 more
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Calcium imaging: a technique to monitor calcium dynamics in biological systems
Physiology and Molecular Biology of Plants, 2023Calcium ion (Ca2+) is a multifaceted signaling molecule that acts as an important second messenger. During the course of evolution, plants and animals have developed Ca2+ signaling in order to respond against diverse stimuli, to regulate a large number of physiological and developmental pathways.
Soma Ghosh +4 more
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Calcium imaging of gut activity
Neurogastroenterology & Motility, 2004AbstractThe major cell types regulating gut motility include enteric neurones, interstitial cells of Cajal (ICC) and their effector smooth muscle cells. These cells are arranged conveniently in nested layers through the gut wall. Our knowledge of how many of these cells in each layer are integrated to produce the various patterns of motility is largely
J, Tack, T K, Smith
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Calcium Imaging in the Zebrafish
2012The zebrafish (Danio rerio) has emerged as a widely used model system during the last four decades. The fact that the zebrafish larva is transparent enables sophisticated in vivo imaging, including calcium imaging of intracellular transients in many different tissues. While being a vertebrate, the reduced complexity of its nervous system and small size
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Calcium Imaging in Mouse Superior Colliculus
Journal of Visualized Experiments, 2023The superior colliculus (SC), an evolutionarily conserved midbrain structure in all vertebrates, is the most sophisticated visual center before the emergence of the cerebral cortex. It receives direct inputs from ~30 types of retinal ganglion cells (RGCs), with each encoding a specific visual feature.
Zhe, Li, Ruixiang, Wu, Ya-Tang, Li
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Ex vivo calcium imaging in Drosophila opens an expansive amount of research avenues for the study of live signal propagation through complex tissue. Here, we describe how to isolate Drosophila organs of interest, like the developing wing imaginal disc and larval brain, culture them for extended periods, up to 10 h, and how to image the calcium dynamics
David V, Gazzo, Jeremiah J, Zartman
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David V, Gazzo, Jeremiah J, Zartman
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Calcium Imaging: A Primer for Mycologists
Fungal Genetics and Biology, 1998This article aims to encourage more fungal biologists to consider the imaging of cytoplasmic Ca2+ fluxes. Compared to other organisms, for fungi there have been remarkably few attempts to characterize the role of Ca2+ fluxes in signal transduction and general cellular activities, even though other approaches indicate that fungal growth and development ...
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Calcium Imaging of Non-adherent Cells
2023Live-cell imaging can reveal dynamic and multimodal cell signaling by monitoring calcium flux. Spatiotemporal changes in Ca2+ concentrations instigate specific downstream processes and by categorizing these events, we can examine the language cells use to communicate both to themselves and with each other.
Bye, Lydia J. +2 more
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Blood cells, 1993
We review herein the following questions related to Ca2+ imaging: what are the key elements to consider in a Ca2+ imaging system? What are the limits of such systems? What are the main artifacts and to what specific applications are a Ca2+ imaging systems best adapted?
A, Trautmann, Y P, Tan
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We review herein the following questions related to Ca2+ imaging: what are the key elements to consider in a Ca2+ imaging system? What are the limits of such systems? What are the main artifacts and to what specific applications are a Ca2+ imaging systems best adapted?
A, Trautmann, Y P, Tan
openaire +1 more source
2003
As our understanding of the biological sciences expands, the boundaries between traditional disciplines tend to blur at the edges. Physiologists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and molecular biologists.
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As our understanding of the biological sciences expands, the boundaries between traditional disciplines tend to blur at the edges. Physiologists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and molecular biologists.
openaire +2 more sources