Results 221 to 230 of about 359,534 (258)
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Calcium imaging in the NIR

Nature Methods, 2021
The near-infrared calcium sensor iGECI shows promise for imaging neuronal activity in vivo.
openaire   +2 more sources

Calcium imaging with chemiluminescence

Microscopy Research and Technique, 1999
This review updates the imaging of free cytosolic calcium with the chemiluminescent aequorins. Basic principles of chemiluminescence are discussed and the biochemistry of aequorins is briefly described. The review provides practical tips on handling and microinjecting aequorins and describes available ultra low light imaging systems.
R, Créton, J A, Kreiling, L F, Jaffe
openaire   +2 more sources

Calcium imaging: a technique to monitor calcium dynamics in biological systems

Physiology and Molecular Biology of Plants, 2023
Calcium ion (Ca2+) is a multifaceted signaling molecule that acts as an important second messenger. During the course of evolution, plants and animals have developed Ca2+ signaling in order to respond against diverse stimuli, to regulate a large number of physiological and developmental pathways.
Soma Ghosh   +4 more
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Calcium imaging of gut activity

Neurogastroenterology & Motility, 2004
AbstractThe major cell types regulating gut motility include enteric neurones, interstitial cells of Cajal (ICC) and their effector smooth muscle cells. These cells are arranged conveniently in nested layers through the gut wall. Our knowledge of how many of these cells in each layer are integrated to produce the various patterns of motility is largely
J, Tack, T K, Smith
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Calcium Imaging in the Zebrafish

2012
The zebrafish (Danio rerio) has emerged as a widely used model system during the last four decades. The fact that the zebrafish larva is transparent enables sophisticated in vivo imaging, including calcium imaging of intracellular transients in many different tissues. While being a vertebrate, the reduced complexity of its nervous system and small size
openaire   +3 more sources

Calcium Imaging in Drosophila

Ex vivo calcium imaging in Drosophila opens an expansive amount of research avenues for the study of live signal propagation through complex tissue. Here, we describe how to isolate Drosophila organs of interest, like the developing wing imaginal disc and larval brain, culture them for extended periods, up to 10 h, and how to image the calcium dynamics
David V, Gazzo, Jeremiah J, Zartman
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Calcium Imaging: A Primer for Mycologists

Fungal Genetics and Biology, 1998
This article aims to encourage more fungal biologists to consider the imaging of cytoplasmic Ca2+ fluxes. Compared to other organisms, for fungi there have been remarkably few attempts to characterize the role of Ca2+ fluxes in signal transduction and general cellular activities, even though other approaches indicate that fungal growth and development ...
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Calcium imaging.

Blood cells, 1993
We review herein the following questions related to Ca2+ imaging: what are the key elements to consider in a Ca2+ imaging system? What are the limits of such systems? What are the main artifacts and to what specific applications are a Ca2+ imaging systems best adapted?
A, Trautmann, Y P, Tan
openaire   +1 more source

Calcium Imaging of Non-adherent Cells

2023
Live-cell imaging can reveal dynamic and multimodal cell signaling by monitoring calcium flux. Spatiotemporal changes in Ca2+ concentrations instigate specific downstream processes and by categorizing these events, we can examine the language cells use to communicate both to themselves and with each other.
Bye, Lydia J.   +2 more
openaire   +3 more sources

Single-Cell Calcium Imaging

2003
As our understanding of the biological sciences expands, the boundaries between traditional disciplines tend to blur at the edges. Physiologists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and molecular biologists.
openaire   +2 more sources

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