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Dopaminergic Regulation of Dendritic Calcium: Fast Multisite Calcium Imaging
2012Optimal dopamine tone is required for the normal cortical function; however it is still unclear how cortical-dopamine-release affects information processing in individual cortical neurons. Thousands of glutamatergic inputs impinge onto elaborate dendritic trees of neocortical pyramidal neurons.
Wen-Liang, Zhou +3 more
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High-Resolution Calcium Imaging Method for Local Calcium Signaling
2019All eukaryotic cells respond to extracellular signals in a physiologically meaningful way. For multicellular organisms, physiologically relevant cooperation is only possible, if cell-cell communication works properly. Here, the extracellular signals must be translated into intracellular signals that ultimately result in cellular responses. This process
Björn-Philipp, Diercks +4 more
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Fluorescence lifetime imaging of intracellular calcium
Journal of Fluorescence, 1993Fluorescence lifetime imaging microscopy (FLIM) is a new methodology for studying the spatial and temporal dynamics of macromolecule, molecules, and ions in living cells. In FLIM image contrast is derived from the mean fluorescence lifetime at each point in a two-dimensional image.
H, Szmacinski +4 more
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Imaging approaches to measuring lysosomal calcium
2015Endolysosomes are emerging as key players that generate as well as respond to intracellular Ca(2+) signals. The role of Ca(2+) in modulating acidic organelle function has long been recognized, but it is now emerging that acidic organelles also act as intracellular Ca(2+) stores; they actively sequester Ca(2+) in their lumina and release it to the ...
Anthony J, Morgan +2 more
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Hybrid Imaging: Calcium Score and Myocardial Perfusion Imaging
Seminars in Nuclear MedicineCoronary heart disease (CHD) remains the top cause of death due to cardiovascular conditions worldwide, with someone suffering a myocardial infarction every 40 seconds. This highlights the importance of non-invasive imaging technologies like myocardial perfusion imaging (MPI), which are crucial for detecting coronary artery disease (CAD) early, even ...
Malek Nayfeh +5 more
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Image Analysis of Calcium Release Dynamics
2008Many aspects of animal development are dependent on the dynamic release of calcium (Ca2+) ions. Although Ca2+ release within a cell is tightly controlled, how the release dynamics result in a specific biological output in embryonic development is less clear.
Christina M, Freisinger +2 more
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1999
Confocal fluorescence microscopy will, in principle, collect light emitted by only that dye that lies in the plane of focus. Signal from dye in regions of the tissue above and below the plane of focus is rejected. Many of us who study intracellular calcium concentration ([Ca++]i) use monolayers of cells in culture. After loading a calcium indicator dye
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Confocal fluorescence microscopy will, in principle, collect light emitted by only that dye that lies in the plane of focus. Signal from dye in regions of the tissue above and below the plane of focus is rejected. Many of us who study intracellular calcium concentration ([Ca++]i) use monolayers of cells in culture. After loading a calcium indicator dye
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Two-photon calcium imaging of neuronal activity
Nature Reviews Methods Primers, 2022Christine Grienberger +2 more
exaly
Imaging of Localized Neuronal Calcium Influx
2003Intracellular Ca2+ controls such diverse processes as growth, cell division, contraction, secretion, and cell death. In neurons Ca2+ influx triggers neurotransmitter release, causes activation of various enzyme cascades, and regulates gene expression.
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