Results 271 to 280 of about 2,798,160 (306)
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Ex vivo calcium imaging in Drosophila opens an expansive amount of research avenues for the study of live signal propagation through complex tissue. Here, we describe how to isolate Drosophila organs of interest, like the developing wing imaginal disc and larval brain, culture them for extended periods, up to 10 h, and how to image the calcium dynamics
David V, Gazzo, Jeremiah J, Zartman
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David V, Gazzo, Jeremiah J, Zartman
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Calcium Imaging: A Primer for Mycologists
Fungal Genetics and Biology, 1998This article aims to encourage more fungal biologists to consider the imaging of cytoplasmic Ca2+ fluxes. Compared to other organisms, for fungi there have been remarkably few attempts to characterize the role of Ca2+ fluxes in signal transduction and general cellular activities, even though other approaches indicate that fungal growth and development ...
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Blood cells, 1993
We review herein the following questions related to Ca2+ imaging: what are the key elements to consider in a Ca2+ imaging system? What are the limits of such systems? What are the main artifacts and to what specific applications are a Ca2+ imaging systems best adapted?
A, Trautmann, Y P, Tan
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We review herein the following questions related to Ca2+ imaging: what are the key elements to consider in a Ca2+ imaging system? What are the limits of such systems? What are the main artifacts and to what specific applications are a Ca2+ imaging systems best adapted?
A, Trautmann, Y P, Tan
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Calcium Imaging of Non-adherent Cells
2023Live-cell imaging can reveal dynamic and multimodal cell signaling by monitoring calcium flux. Spatiotemporal changes in Ca2+ concentrations instigate specific downstream processes and by categorizing these events, we can examine the language cells use to communicate both to themselves and with each other.
Bye, Lydia J. +2 more
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Dopaminergic Regulation of Dendritic Calcium: Fast Multisite Calcium Imaging
2012Optimal dopamine tone is required for the normal cortical function; however it is still unclear how cortical-dopamine-release affects information processing in individual cortical neurons. Thousands of glutamatergic inputs impinge onto elaborate dendritic trees of neocortical pyramidal neurons.
Wen-Liang, Zhou +3 more
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High-Resolution Calcium Imaging Method for Local Calcium Signaling
2019All eukaryotic cells respond to extracellular signals in a physiologically meaningful way. For multicellular organisms, physiologically relevant cooperation is only possible, if cell-cell communication works properly. Here, the extracellular signals must be translated into intracellular signals that ultimately result in cellular responses. This process
Björn-Philipp, Diercks +4 more
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2003
As our understanding of the biological sciences expands, the boundaries between traditional disciplines tend to blur at the edges. Physiologists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and molecular biologists.
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As our understanding of the biological sciences expands, the boundaries between traditional disciplines tend to blur at the edges. Physiologists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and molecular biologists.
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Imaging approaches to measuring lysosomal calcium
2015Endolysosomes are emerging as key players that generate as well as respond to intracellular Ca(2+) signals. The role of Ca(2+) in modulating acidic organelle function has long been recognized, but it is now emerging that acidic organelles also act as intracellular Ca(2+) stores; they actively sequester Ca(2+) in their lumina and release it to the ...
Anthony J, Morgan +2 more
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Hybrid Imaging: Calcium Score and Myocardial Perfusion Imaging
Seminars in Nuclear MedicineCoronary heart disease (CHD) remains the top cause of death due to cardiovascular conditions worldwide, with someone suffering a myocardial infarction every 40 seconds. This highlights the importance of non-invasive imaging technologies like myocardial perfusion imaging (MPI), which are crucial for detecting coronary artery disease (CAD) early, even ...
Malek Nayfeh +5 more
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1999
Confocal fluorescence microscopy will, in principle, collect light emitted by only that dye that lies in the plane of focus. Signal from dye in regions of the tissue above and below the plane of focus is rejected. Many of us who study intracellular calcium concentration ([Ca++]i) use monolayers of cells in culture. After loading a calcium indicator dye
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Confocal fluorescence microscopy will, in principle, collect light emitted by only that dye that lies in the plane of focus. Signal from dye in regions of the tissue above and below the plane of focus is rejected. Many of us who study intracellular calcium concentration ([Ca++]i) use monolayers of cells in culture. After loading a calcium indicator dye
openaire +1 more source