Results 161 to 170 of about 4,652 (211)
Immunoblotting for Calpastatin Expression
, 2019 Immunoblotting is a procedure routinely used to analyze calpastatin expression. However, immunoblotting alone may not be adequate for this task, since calpastatin isoforms can vary by tissue, can be modified by partial digestion, and can undergo posttranslational modifications.
Averna, Monica, De Tullio, Roberta
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Isolation of Endogenous Calpastatin
, 2019 We here describe the purification of calpastatin from human erythrocytes. When calpastatin is purified from tissues, it is necessary to measure its inhibitory activity against calpain in the presence of Ca2+ to specifically identify the protein. Thus, the purification steps necessary to obtain the inhibitor protein were originally designed to obtain ...
De Tullio, Roberta, Averna, Monica
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Immunoaffinity purification of calpastatin and calpastatin constructs
BBA - Proteins and Proteomics, 2002It has been difficult to purify calpastatin without using a step involving heating to 90-100 degrees C. Preparations of calpastatin obtained after heating often contain several polypeptides that have been ascribed to proteolytic degradation. Because calpastatin is highly susceptible to proteolytic degradation and several different calpastatin isoforms ...
Valery F Thompson, Darrel E Goll
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Production and Purification of Recombinant Calpastatin
, 2019 The production of recombinant calpastatin in E. coli has become an efficient tool to obtain discrete amounts of a specific calpastatin species that can be present concomitantly with other calpastatin fragments/forms in the same tissue or cell type in a given condition.
De Tullio, Roberta, Averna, Monica
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Correlation between a novel calpastatin biosensor and traditional calpastatin assay techniques
Biosensors and Bioelectronics, 2008An optical fiber biosensor to detect calpastatin has been investigated as a preliminary step in developing tenderness detection instrumentation. Longissimus dorsi samples were taken from beef carcasses (n=21) at 0, 24, 36 and 48h postmortem. Muscle homogenates were assayed for calpastatin activity using traditional methods and an optical fiber ...
Sheila A Grant, C L Lorenzen
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Expression of calpastatin isoforms in muscle and functionality of multiple calpastatin promoters
Archives of Biochemistry and Biophysics, 2004 Calpastatin is the specific endogenous inhibitor of calpain proteinase that is encoded by a single gene. Transient transfection assays in both a non-fusing skeletal muscle and non-muscle cell-line demonstrated that the putative porcine calpastatin promoter regions 5' to exons 1xa, 1xb, and 1u were functional.
Tim, Parr +5 more
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Calpastatin level in spontaneously hypertensive rats
Biochemical and Biophysical Research Communications, 1991 We studied calpastatin activity in erythrocytes of Milan hypertensive and prehypertensive rats, in their normotensive controls, in F1 and F2 hybrids, and in two inbred strains derived from F2, one hypertensive and the other normotensive. Our results show that the decrease in calpastatin activity observed in Milan hypertensive rats was not caused by ...
L. Soldati +6 more
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Biochemical and Biophysical Research Communications, 2008
Calpastatin is an endogenous inhibitor of calpain, which has been implicated in various physiological and pathological processes. In the present study we determined the molecular and inhibitory properties of HMWCaMBP, calpastatin I, and calpastatin II. Western blot analysis with antibodies raised against either full length HMWCaMBP or internal peptides
Nisha Singh +2 more
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Calpastatin is an endogenous inhibitor of calpain, which has been implicated in various physiological and pathological processes. In the present study we determined the molecular and inhibitory properties of HMWCaMBP, calpastatin I, and calpastatin II. Western blot analysis with antibodies raised against either full length HMWCaMBP or internal peptides
Nisha Singh +2 more
exaly +3 more sources
Calpain–calpastatin system and cancer progression
Biological Reviews, 2021ABSTRACTThe calpain system is required by many important physiological processes, including the cell cycle, cytoskeleton remodelling, cellular proliferation, migration, cancer cell invasion, metastasis, survival, autophagy, apoptosis and signalling, as well as the pathogenesis of a wide range of disorders, in which it may function to promote ...
Hong Nian, Binyun Ma
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Interaction of calpastatin with calpain: a review
Biological Chemistry, 2004Calpastatin is a multiheaded inhibitor capable of inhibiting more than one calpain molecule. Each inhibitory domain of calpastatin has three subdomains, A, B, and C; A binds to domain IV and C binds to domain VI of the calpains. Crystallographic evidence shows that binding of C to domain VI involves hydrophobic interactions at a site near the first EF ...
Amanda, Wendt +2 more
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