Improvement of EPO Transgene Detection From Polymeric Dried Blood Spots for Antidoping Application
This article presents an optimized protocol for gene doping detection. Starting from one spot DBS (20 μL), concentrated DNA was obtained and real‐time PCR parameters were adjusted for increased detection. Robust detection of 5000 copies/mL of transgene was validated for polymeric device TASSO M‐20.
Alexandre Marchand +2 more
wiley +1 more source
Characterization of the Specific Binding Between Aptamers and Cytochrome c With Pressure-Assisted Capillary Electrophoresis Frontal Analysis. [PDF]
Wang S, Li C, Wang S, Li H, Chen DDY.
europepmc +1 more source
Abstract Background Sporadic venous malformation (VM) is associated with the hyperactivating p.L914F mutation in TIE2, a receptor tyrosine kinase essential for vascular development. This mutation is not found in hereditary VM, suggesting incompatibility with life when expressed during early vascular development.
Lindsay J. Bischoff +6 more
wiley +1 more source
Chiral separation of aromatic amino acids by capillary electrophoresis using sulphated β-cyclodextrin as a single chiral selector: an experimental and computational study. [PDF]
Elbashir AA +3 more
europepmc +1 more source
This review focuses on the mechanisms of Zn2+ electrodeposition and interfacial failure together with systematical artificial interphase layer design strategies from carbon materials, zincophilic alloys, to inorganic/organic compound layers, regulating ion flux and desolvation and providing guidance for programmable interface construction in aqueous ...
Bixian Chen +12 more
wiley +1 more source
Impacts of Tween-20, Glycerol, and Trehalose on Hyaluronidase Activity: Insights from Microscale Thermophoresis and Capillary Electrophoresis. [PDF]
Nasreddine R +3 more
europepmc +1 more source
Sustainability in Analytical Chemistry Illustrated by Pharmaceutical Nitrosamine Testing
ABSTRACT Following the valsartan scandal in 2018, the testing of drug substances and drug products for N‐nitrosamines has become a critical and mandatory quality control measure. The European Pharmacopoeia chapter 2.5.42 currently describes three analytical methods for this purpose: HPLC–MS/MS, GC–MS, and GC–MS/MS.
Felix Bredendiek +2 more
wiley +1 more source
Quantification of Interactions between Small Molecules and RNA Probes Representative of Myotonic Dystrophy Type 1 Using Affinity Capillary Electrophoresis and UPLC-UV. [PDF]
Leveque M +5 more
europepmc +1 more source

