Results 161 to 170 of about 298,386 (364)

Adaptive Machine Learning Framework for Optimizing the Affinity Purification of Adeno‐Associated Viral Vectors

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Adeno‐associated viral (AAV) vectors for gene therapy are becoming integral to modern medicine, providing therapeutic options for diseases once deemed incurable. Currently, viral vector purification is a critical bottleneck in the gene therapy industry, impacting product efficacy and safety as well as accessibility and cost to patients ...
Kelvin P. Idanwekhai, Shriarjun Shastry, Morgan R. Hurst, Arianna Minzoni, Eduardo Barbieri, Luke Remmler, Eugene N. Muratov, Michael A. Daniele, Stefano Menegatti, Alexander Tropsha   +9 more
wiley   +1 more source

Applying Derivative-Free Optimization to Fit Kinetic Parameters of Viral Capsid Self-Assembly Models from Multi-Source Bulk in vitro Data [PDF]

, 2015
Lu Xie, Gregory R. Smith, Russell Schwartz   +2 more
openalex   +1 more source

Performance of large virus removal filters during AAV processing: Influence of flux and process disruptions

Biotechnology Progress, EarlyView.
Abstract As adeno‐associated viral vectors (AAV) continue to advance through the clinical pipeline, effective downstream purification strategies must be developed to ensure bulk drug purity and safety. AAV are produced within mammalian cells, bringing forth risks associated with viral contamination.
Akshay S. Chaubal, Asingsa W. Arachchige, Annabelle J. Zahn, S. Ranil Wickramasinghe, Xianghong Qian, Andrew L. Zydney   +5 more
wiley   +1 more source

3.5Å cryoEM structure of hepatitis B virus core assembled from full-length core protein. [PDF]

, 2013
The capsid shell of infectious hepatitis B virus (HBV) is composed of 240 copies of a single protein called HBV core antigen (HBc). An atomic model of a core assembled from truncated HBc was determined previously by X-ray crystallography.
Jih, Jonathan, Jin, Lei, Shih, Chiaho, Yu, Xuekui, Zhou, Z Hong   +4 more
core  

Diversity, transfer potential, and transcriptional activity of virus‐carried antibiotic resistance genes in global estuaries

iMetaOmics, EarlyView.
Estuaries are vital hotspots for antibiotic resistance genes (ARGs) due to substantial antibiotic pollution. Although viruses have been proposed as key reservoirs and important disseminators of ARGs in environments, their contribution to the estuarine antibiotic resistome remains largely unknown.
Xiao‑Qing Luo, Xiyang Dong, Jia‑Ling Li, Ji‐Cheng Yao, Ziqi Peng, Qi Li, Zi‐Wen Yang, Yang Yuan, Wen‑Jun Li, Pandeng Wang   +9 more
wiley   +1 more source

Direct Detection of Viruses Using Antibody‐Modified Gold Nanorods

Journal of Biophotonics, EarlyView.
Gold nanorods functionalized with pathogen‐specific antibodies can be used for the confirmatory detection of viruses via dynamic light scattering–based analysis of particle sizes. ABSTRACT Seasonal and sporadic viral infections put enormous burdens on global health and society. Although polymerase chain reaction (PCR) and other molecular approaches can
Axell Rodriguez, Yana Purvinsh, Aidan P. Holman, Dmitry Kurouski   +3 more
wiley   +1 more source

Biophysical and Ultrastructural Characterization of Adeno-Associated Virus Capsid Uncoating and Genome Release [PDF]

, 2012
Eric D. Horowitz, Kazi Shefaet Rahman, Brian D. Bower, David Dismuke, Michael R. Falvo, Jack D. Griffith, Stephen C. Harvey, Aravind Asokan   +7 more
openalex   +1 more source

Capture of adeno‐associated viruses from clarified lysate by continuous flow ultracentrifugation: a comparative study of iodixanol and sucrose gradients

Journal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND Adeno‐Associated Virus vectors are widely used in gene therapy due to their safety and efficacy. Despite the interest in their therapeutical application, AAV purification remains a significant challenge. Density gradient ultracentrifugation using cesium chloride, sucrose, or iodixanol gradients are common methods for AAV ...
Sabrina Leigheb, Maral Rahimzadeh, Nikolaus Virgolini, Aida Tleuova, Mahbod Mousavian, Astrid Dürauer, Patricia Pereira Aguilar   +6 more
wiley   +1 more source

From the Cytoplasm into the Nucleus—Hepatitis B Virus Travel and Genome Repair

Microorganisms
Hepatitis B virus (HBV) is a major global health concern, affecting millions of people worldwide. HBV is part of the hepadnaviridae family and one of the primary causes of acute and chronic liver infections, leading to conditions such as cirrhosis and ...
Johan Ringlander, Gustaf E. Rydell, Michael Kann   +2 more
doaj   +1 more source

Self-assembly of virus-like particles of porcine circovirus type 2 capsid protein expressed from Escherichia coli [PDF]

, 2010
Shuanghui Yin, Shiqi Sun, Shunli Yang, Youjun Shang, Xuepeng Cai, Xiangtao Liu   +5 more
openalex   +1 more source