Results 91 to 100 of about 812,191 (301)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Cell membrane patches transfer CAR molecules from a cellular depot to conventional T cells for constructing innovative fused-CAR-T cells without necessitating genetic modification

Experimental Hematology & Oncology
Chimeric antigen receptor (CAR) serves as the foundational element of CAR-T cells. Exogenous CAR molecules can exert functional effects on allogeneic T cells, leading to their activation and subsequent functional alterations.
Jing Hu, Luyi Zhong, Yiqiu Wang, Shiyi Hu, Lijiaqi Zhang, Qingchang Tian   +5 more
doaj   +1 more source

Tisagenlecleucel in children and young adults with B-cell lymphoblastic leukemia [PDF]

, 2018
BACKGROUND: In a single-center phase 1-2a study, the anti-CD19 chimeric antigen receptor (CAR) T-cell therapy tisagenlecleucel produced high rates of complete remission and was associated with serious but mainly reversible toxic effects in children and ...
Bader, P, Balduzzi, A, Baruchel, A, Bittencourt, H, Boissel, N, Boyer, M, Buechner, J, Davis, KL, De Moerloose, Barbara, Grupp, SA, Hiramatsu, H, June, CH, Krueger, J, Laetsch, TW, Lebwohl, D, Leung, M, Levine, BL, Martin, PL, Maude, SL, Mechinaud, F, Mueller, KT, Myers, GD, Nemecek, ER, Peters, C, Pulsipher, MA, Qayed, M, Rives, S, Schlis, K, Sen, K, Stefanski, HE, Taran, T, Verneris, MR, Wood, P, Yanik, GA, Zhang, Y   +34 more
core   +2 more sources

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

A Cross-Reactive Small Protein Binding Domain Provides a Model to Study Off-Tumor CAR-T Cell Toxicity

Molecular Therapy: Oncolytics, 2020
Tumor-targeted chimeric antigen receptor (CAR)-engineered T lymphocytes (CAR-T cells) have demonstrated striking clinical success, but their use has been associated with a constellation of toxicities.
Joanne A. Hammill, Jacek M. Kwiecien, Anna Dvorkin-Gheva, Vivian W.C. Lau, Christopher Baker, Ying Wu, Ksenia Bezverbnaya, Craig Aarts, Christopher W. Heslen, Galina F. Denisova, Heather Derocher, Katy Milne, Brad H. Nelson, Jonathan L. Bramson   +13 more
doaj   +1 more source

Digital twins to accelerate target identification and drug development for immune‐mediated disorders

FEBS Open Bio, EarlyView.
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley   +1 more source

The application of CAR-T cell therapy in hematological malignancies: advantages and challenges

Acta Pharmaceutica Sinica B, 2018
Chimeric antigen receptor T cell (CAR-T cell) therapy is a novel adoptive immunotherapy where T lymphocytes are engineered with synthetic receptors known as chimeric antigen receptors (CAR).
Zijun Zhao, Yu Chen, Ngiambudulu M. Francisco, Yuanqing Zhang, Minhao Wu   +4 more
doaj   +1 more source

Low interleukin-2 concentration favors generation of early memory T cells over effector phenotypes during chimeric antigen receptor T-cell expansion [PDF]

, 2017
Errata: 10.1016/j.jcyt.2017.06.003Background. Adoptive T-cell therapy offers new options for cancer treatment. Clinical results suggest that T-cell persistence, depending on T-cell memory, improves efficacy.
Arvas, Mikko, Belt, Heini, Kaartinen, Tanja, Keto, Joni, Koponen, Jonna, Korhonen, Matti, Loskog, Angelica, Luostarinen, Annu, Maliniemi, Pilvi, Mustjoki, Satu, Porkka, Kimmo, Yla-Herttuala, Seppo   +11 more
core   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source