Results 41 to 50 of about 132,531 (238)

CBMDB: A Database for Accessing, Analyzing, and Mining CBM Information

Applied Sciences, 2022
Carbohydrate-binding modules (CBMs) are important substrate-binding domains that are mainly contained within carbohydrate-active enzymes. To elucidate the mechanism of enzyme-carbohydrate recognition and to promote the process of enzymatic engineering ...
Xu Lin, Xiaoqi Xie, Xueyan Wang, Zhimin Yu, Xiaoyi Chen, Fan Yang   +5 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Rahnella sp., a Dominant Symbiont of the Core Gut Bacteriome of Dendroctonus Species, Has Metabolic Capacity to Degrade Xylan by Bifunctional Xylanase-Ferulic Acid Esterase

Frontiers in Microbiology, 2022
Rahnella sp. ChDrAdgB13 is a dominant member of the gut bacterial core of species of the genus Dendroctonus, which is one of the most destructive pine forest bark beetles. The objectives of this study were identified in Rahnella sp. ChDrAdgB13 genome the
Rosa María Pineda-Mendoza, Gerardo Zúñiga, María Fernanda López, María Eugenia Hidalgo-Lara, Alejandro Santiago-Hernández, Azucena López-López, Flor N. Rivera Orduña, Claudia Cano-Ramírez   +7 more
doaj   +1 more source

Carbohydrate‐active enzymes exemplify entropic principles in metabolism

Molecular Systems Biology, 2011
Glycans comprise ubiquitous and essential biopolymers, which usually occur as highly diverse mixtures. The myriad different structures are generated by a limited number of carbohydrate-active enzymes (CAZymes), which are unusual in that they catalyze multiple reactions by being relatively unspecific with respect to substrate size. Existing experimental
O. Kartal, S. Mahlow, A. Skupin, O. Ebenhoh   +3 more
openaire   +6 more sources

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

Multimodular fused acetyl–feruloyl esterases from soil and gut Bacteroidetes improve xylanase depolymerization of recalcitrant biomass

Biotechnology for Biofuels, 2020
Background Plant biomass is an abundant and renewable carbon source that is recalcitrant towards both chemical and biochemical degradation. Xylan is the second most abundant polysaccharide in biomass after cellulose, and it possesses a variety of ...
Cathleen Kmezik, Cyrielle Bonzom, Lisbeth Olsson, Scott Mazurkewich, Johan Larsbrink   +4 more
doaj   +1 more source

Reliable and Scalable Identification and Prioritization of Putative Cellulolytic Anaerobes With Large Genome Data

Frontiers in Bioinformatics, 2022
In the era of high-throughput sequencing, genetic information that is inherently whispering hints of the microbes’ functional niches is becoming easily accessible; however, properly identifying and characterizing these genetic hints to infer the microbes’
Yubo Wang, Liguan Li, Yu Xia, Yu Xia, Tong Zhang, Tong Zhang, Tong Zhang   +6 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Genome data mining of a novel Stutzerimonas marianensis strain LB-0542 isolated from pelagic Sargassum seaweed waste for plastic-degrading and plant growth-promoting traitsGenBank-National Center for Biotechnology Information

Data in Brief
This study reports the whole-genome sequence data and functional annotations of a novel Stutzerimonas marianensis strain LB-0542 isolated from the decomposing pelagic Sargassum biomass stranded on Long Beach, Barbados.
Bidyut R. Mohapatra, Linel S. Moralez, Kiya E. James   +2 more
doaj   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source