Results 271 to 280 of about 93,083 (312)

Gene transfer in cardiac myocytes

Surgical Clinics of North America, 2004
Congestive heart failure (CHF) represents an enormous clinical problem and remains a leading cause of death despite advances in treatment. New treatments significantly impact mortality and disease course; they do not cure the underlying pathology. Gene transfer, the ability to genetically reprogram the heart in relevant cardiovascular disease models ...
Babar B, Chaudhri, Federica, del Monte, Sian E, Harding, Roger J, Hajjar   +3 more
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Electrical stimulation of cardiac myocytes

Annals of Biomedical Engineering, 1995
The influence of nonuniform cell shape and field orientation on the field stimulation thresholds of cardiac myocytes was studied both experimentally and computationally. The percent change in excitation threshold, which was studied with patch clamp technique, was found to be 182 +/- 83.1% (mean +/- SD) higher when the electric field (EF) was parallel ...
R, Ranjan, N V, Thakor
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How to isolate cardiac myocytes

Cardiovascular Research, 1994
A complete technique is described for the isolation of myocytes from mammalian hearts using the Langendorff perfusion technique. The use of calcium-free solution containing collagenase and protease, followed by low calcium solution, consistently results in a large number of calcium tolerant myocytes which are well suited for long periods of ...
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Cardiac Myocyte Terminal Differentiation

Annals of the New York Academy of Sciences, 1995
The exact mechanism of terminal differentiation in cardiac myocytes is currently unknown. Studies in the skeletal muscle system provided a model where muscle lineage termination gene directly interacts with Rb to produce and maintain the terminally differentiated state. This interaction provided the critical components for the lock in cell cycle arrest
S K, Tam, W, Gu, V, Mahdavi, B, Nadal-Ginard   +3 more
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Confocal Microscopy of Cardiac Myocytes

2013
Detailed methods are provided for the preparation and confocal imaging of cardiac myocyte development and differentiation. Examples include protocols for the analysis of cultured myocytes as well as vibratome sections of hearts from embryonic and adult tissue.
Robert L, Price, Stephen T, Haley, Tara, Bullard, Jeffrey, Davis, Thomas K, Borg, Louis, Terracio   +5 more
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Scaffolding Proteins in Cardiac Myocytes

2008
Post-translational modification, such as protein phosphorylation, plays a critical role to reversibly amplify and modulate signaling pathways. Since kinases and phosphatases have broad substrate recognition motifs, compartmentalization and localization of signaling complexes are required to achieve specific signals.
N L, Chudasama, S O, Marx, S F, Steinberg   +2 more
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Adrenergic regulation of cardiac myocyte apoptosis

Journal of Cellular Physiology, 2001
AbstractThe direct effects of catecholamines on cardiac myocytes may contribute to both normal physiologic adaptation and pathologic remodeling, and may be associated with cellular hypertrophy, apoptosis, and alterations in contractile function.
K, Singh, L, Xiao, A, Remondino, D B, Sawyer, W S, Colucci   +4 more
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Calcium Signaling in Cardiac Ventricular Myocytes

Annals of the New York Academy of Sciences, 2005
Abstract: Calcium (Ca) is a multifunctional regulator of diverse cellular functions. In cardiac muscle Ca is a direct central mediator of electrical activation, ion channel gating, and excitation‐contraction (E‐C) coupling that all occur on the millisecond time scale. The key amplification step in E‐C coupling is under tight control of very local [Ca].
Donald M, Bers, Tao, Guo
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Regulation of cardiac myocyte cell death

Pharmacology & Therapeutics, 2003
Cardiac myocyte death, whether through necrotic or apoptotic mechanisms, is a contributing factor to many cardiac pathologies. Although necrosis and apoptosis are the widely accepted forms of cell death, they may utilize the same cell death machinery. The environment within the cell probably dictates the final outcome, producing a spectrum of response ...
Angela, Clerk, Sharon M, Cole, Timothy E, Cullingford, Joanne G, Harrison, Mika, Jormakka, Donna M, Valks   +5 more
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Electrophysiology of cardiac myocytes of Aplysia brasiliana

Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2002
The electrical properties of Aplysia brasiliana myogenic heart were evaluated. Two distinct types of action potentials (APs) were recorded from intact hearts, an AP with a slow rising phase followed by a slow repolarizing phase and an AP with a 'fast' depolarizing phase followed by a plateau.
Marta M, Souza, Arlete, Stucchi-Zucchi, Antonio C, Cassola, Eliana, Scemes   +3 more
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