Results 171 to 180 of about 234,827 (207)

Microtopographical assembly of cardiomyocytes

Integrative Biology, 2011
One of the central challenges in cardiac tissue engineering is the control of the assembly and organization of functional cardiac tissue. Maintenance of a three-dimensional tissue architecture is key to myocardial function in vivo, and a variety of studies hint that provision of topological cues within scaffolds can facilitate the engineering of ...
Anuj A, Patel, Tejal A, Desai, Sanjay, Kumar   +2 more
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Glycation in the cardiomyocyte

Glycation is a protein post-translational modification that can occur on lysine and arginine residues as a result of a non-enzymatic process known as the Maillard reaction. This modification is irreversible, so the only way it can be removed is by protein degradation and replacement.
Christine E, Delligatti, Jonathan A, Kirk   +1 more
openaire   +2 more sources

Isolation and Differentiation of Human Cardiomyocyte Progenitor Cells into Cardiomyocytes

2012
To date, there is no suitable in vitro model to study human adult cardiac cell biology. Here, we describe a method for efficient isolation and expansion of human cardiomyocyte progenitor cells (CMPCs) from cardiac surgical waste or, alternatively, from fetal heart tissue.
Anke M, Smits, Angelique A, van Oorschot, Marie-José, Goumans   +2 more
openaire   +2 more sources

Pathophysiology of Cardiomyocytes^a

Annals of the New York Academy of Sciences, 1988
Isolated cardiomyocytes lend themselves very well to the quantification of pathological damage and to the determination of reversible versus irreversible changes. These single cells were used to study the cellular response to a variety of pathologic stimuli that impair structure and function.
M, Borgers, L, Ver Donck, G, Vandeplassche   +2 more
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Transdifferentiation of mesenchymal stem cells into cardiomyocytes by direct cell-to-cell contact with neonatal cardiomyocyte but not adult cardiomyocytes

Annals of Hematology, 2005
Recent studies have demonstrated that direct cell-to-cell interaction is one of the microenvironment factors for transdifferentiation of adult stem cells into cardiomyocytes. We investigated whether transdifferentiation of mesenchymal stem cells (MSCs) into cardiomyocytes was dependent on developmental stages of cocultured cardiomyocytes, and direct ...
Do-Sun Lim
exaly   +3 more sources

Cryopreservation of Neonatal Cardiomyocytes

2015
Cardiomyocytes are frequently used for in vitro models for cardiac research. The isolation of cells is time-consuming and, due to the cells limited proliferative abilities, must be performed frequently. To reduce the time requirements and the impact on research animals, we describe a method for cryopreserving neonatal rat cardiomyocytes (NRCMs), and ...
Adam C, Vandergriff, M Taylor, Hensley, Ke, Cheng   +2 more
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Differentiation of Cardiomyocytes in Floating Embryoid Bodies is Comparable to Fetal Cardiomyocytes

Journal of Molecular and Cellular Cardiology, 2000
Embryonic stem cells will cluster and differentiate into embryoid bodies, which can develop spontaneous rhythmic contractions. From these embryoid bodies, cardiomyocytes can be isolated based on density by a discontinuous Percoll gradient. These cardiomyocytes differentiate into ventricular myocytes, which is demonstrated by the expression of the ...
Pieter A Doevendans, Kenneth R Chien
exaly   +3 more sources

The electrophysiological development of cardiomyocytes

Advanced Drug Delivery Reviews, 2016
The generation of human cardiomyocytes (CMs) from human pluripotent stem cells (hPSCs) has become an important resource for modeling human cardiac disease and for drug screening, and also holds significant potential for cardiac regeneration. Many challenges remain to be overcome however, before innovation in this field can translate into a change in ...
Jie, Liu, Zachary, Laksman, Peter H, Backx   +2 more
openaire   +2 more sources

Doxorubicin Degradation in Cardiomyocytes

The Journal of Pharmacology and Experimental Therapeutics, 2007
Antitumor therapy with the anthracycline doxorubicin is limited by a severe cardiotoxicity, which seems to correlate with the cardiac levels of doxorubicin and its metabolization to reactive oxygen species. Previous biochemical studies showed that hydrogen peroxide-activated myoglobin caused an oxidative degradation of doxorubicin; however, a ...
Pierantonio, Menna, Emanuela, Salvatorelli, Giorgio, Minotti   +2 more
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Resuscitation of a dead cardiomyocyte

Heart Failure Reviews, 2015
Although cardiac resuscitation can revive the whole body, the mechanisms are unclear. To this end, we propose that reviving a dead/dysfunctional cardiomyocyte will shed light on resuscitation mechanisms and pave the way to treat cardiac myopathies. The degradation of the myocyte cytoskeleton by the proteasome system which involves calpains, ubiquitin ...
George H, Kunkel, Pankaj, Chaturvedi, Suresh C, Tyagi   +2 more
openaire   +2 more sources