Results 111 to 120 of about 194,133 (208)

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

, 2013
Kevin M. Esvelt, Prashant Mali, Jonathan L. Braff, Mark Moosburner, Stephanie J. Yaung, George M. Church   +5 more
openalex   +2 more sources

crRNA and tracrRNA guide Cas9-mediated DNA interference inStreptococcus thermophilus

, 2013
Tautvydas Karvelis, Giedrius Gasiūnas, Algirdas Miksys, Rodolphe Barrangou, Philippe Horvath, Virginijus Šikšnys   +5 more
openalex   +1 more source

Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production

Nature Communications
The CRISPR-Cas9 system has frequently been used for genome editing in Streptomyces; however, cytotoxicity, caused by off-target cleavage, limits its application.
Duck Gyun Kim, Boncheol Gu, Yujin Cha, Jeonghan Ha, Yongjae Lee, Gahyeon Kim, Byung-Kwan Cho, Min-Kyu Oh   +7 more
doaj   +1 more source

Enhanced Specificity and Efficiency of the CRISPR/Cas9 System with Optimized sgRNA Parameters in Drosophila [PDF]

, 2014
Xingjie Ren, Zhihao Yang, Xu Jiang, Jin Sun, Decai Mao, Yanhui Hu, Sujuan Yang, Huanhuan Qiao, Xia Wang, Qun Hu, Patricia Deng, Luping Liu, Jun‐Yuan Ji, Jin Billy Li, Jian‐Quan Ni   +14 more
openalex   +1 more source

Targeting non-coding RNAs with the CRISPR/Cas9 system in human cell lines [PDF]

, 2014
Tsui-Ting Ho, Nanjiang Zhou, Jianguo Huang, Pratirodh Koirala, Min Xu, Roland Fung, Fangting Wu, Yin‐Yuan Mo   +7 more
openalex   +1 more source

Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification

Molecular Therapy: Nucleic Acids, 2018
The Cas9/guide RNA (Cas9/gRNA) system is commonly used for genome editing. mRNA expressing Cas9 can induce innate immune responses, reducing Cas9 expression.
Sriram Vaidyanathan, Krist T. Azizian, A.K.M. Ashiqul Haque, Jordana M. Henderson, Ayal Hendel, Sabrina Shore, Justin S. Antony, Richard I. Hogrefe, Michael S.D. Kormann, Matthew H. Porteus, Anton P. McCaffrey   +10 more
doaj  

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

Nature, 2014
S. Konermann, M. Brigham, Alexandro E. Trevino, J. Joung, Omar O. Abudayyeh, Clea Bárcena, P. Hsu, Naomi Habib, J. Gootenberg, H. Nishimasu, O. Nureki, Feng Zhang   +11 more
semanticscholar   +1 more source

Efficient and Heritable Gene Targeting in Tilapia by CRISPR/Cas9 [PDF]

, 2014
Minghui Li, Hui‐Hui Yang, Jiue Zhao, Lingling Fang, Hongjuan Shi, Mengru Li, Yun-Lv Sun, Xianbo Zhang, Dongneng Jiang, Linyan Zhou, Deshou Wang   +10 more
openalex   +1 more source

CRISPRs extending their reach: prokaryotic RNAi protein Cas9 recruited for gene regulation

, 2013
Nadja Heidrich, Jörg Vogel
openalex   +2 more sources

Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells [PDF]

, 2014
Yilan Zhang, Xianglian Ge, Fayu Yang, Ping Zhang, Jiayong Zheng, Xuefang Tan, Zi‐Bing Jin, Jia Qu, Feng Gu   +8 more
openalex   +1 more source