Results 121 to 130 of about 200,296 (309)
CRISPR Cas9 in Pancreatic Cancer Research
Frontiers in Cell and Developmental Biology, 2019 Pancreatic cancer is now becoming a common cause of cancer death with no significant change in patient survival over the last 10 years. The main treatment options for pancreatic cancer patients are surgery, radiation therapy and chemotherapy, but there ...
Hai Yang +2 more
doaj +1 more source
Genes Linked to Production of Secondary Metabolites in Talaromyces atroroseus Revealed Using CRISPR-Cas9 [PDF]
, 2017 The full potential of fungal secondary metabolism has until recently been impeded by the lack of universal genetic tools for most species. However, the emergence of several CRISPR-Cas9-based genome editing systems adapted for several genera of ...
Maria Lund Nielsen +20 more
core +1 more source
Advanced Science, EarlyView.TRMT6‐mediated m1A modification in CDK9 mRNA enhances its mRNA stability and translation efficiency, thereby increasing the protein levels of CDK9. Upregulated CDK9 promotes the progression of HCC by elevating the levels of oncogenic factors including p‐STAT3, MCL1, and BCL‐2. On the other hand, CDK9 phosphorylates TARDBP at Ser254 to activate HBV core
Rui Zhang +12 more
wiley +1 more source
Optimized Plasmid Construction Strategy for Cas9
Cellular Physiology and Biochemistry, 2018 Background/Aims: The target genome editing technology not only plays an important role in basic biology studies but also holds a great promise for potential clinical applications.
Jianyong Xu +5 more
doaj +1 more source
Improved Cas9 activity by specific modifications of the tracrRNA
, 2019 CRISPR/Cas is a transformative gene editing tool, that offers a simple and effective way to target a catalytic Cas9, the most widely used is derived from Streptococcus pyogenes (SpCas9), with a complementary small guide RNA (sgRNA) to inactivate ...
Ryan Urak +7 more
core +1 more source
Advanced Science, EarlyView.A genome‐wide microRNA CRISPR screen identifies miR‐18a as a master regulator of cross‐resistance in melanoma. Loss of miR‐18a activates the AJUBA–YAP/Hippo axis to confer BRAFi resistance and enhances THBS1–CD47 interaction to impair CD8+ T cell immunity. hnRNP A1 is identified as an upstream regulator of miR‐18a processing.
Zhao Wang +19 more
wiley +1 more source
Adipocyte‐Derived Leptolin Enhances Energy Expenditure and Prevents Obesity
Advanced Science, EarlyView.We identified a novel adipokine, which we named leptolin. In humans, leptolin levels in white adipose tissue were positively correlated with exercise and negatively associated with body mass index. We observed elevated leptolin in serum from athletes and lower leptolin in serum from obese individuals.
Jiarui Liu +17 more
wiley +1 more source
Enhanced Biosynthesis Performance of Heterologous Proteins in CHO-K1 Cells Using CRISPR-Cas9
, 2018 Chinese hamster ovary (CHO) cells are the famous expression system for industrial production of recombinant proteins, such as therapeutic antibodies. However, there still remain bottlenecks in protein quality and weakness in expression efficiency because
Wenpeng Wang (5147411) +7 more
core +1 more source
A Rare Allele of ST5 From Wild Rice Enhances Salt Tolerance in Rice
Advanced Science, EarlyView.A novel salt‐tolerance gene ST5W, characterized by a unique 36‐bp promoter insertion, is found exclusively in a small subset of Oryza rufipogon and is absent in cultivated rice. Field trials confirm ST5W significantly enhances rice yield across diverse genetic backgrounds under saline conditions.
Meng Xing +26 more
wiley +1 more source
In vivo evaluation of guide-free Cas9-induced safety risks in a pig model
Signal Transduction and Targeted TherapyThe CRISPR/Cas9 system has shown great potential for treating human genetic diseases through gene therapy. However, there are concerns about the safety of this system, specifically related to the use of guide-free Cas9.
Weikai Ge +14 more
doaj +1 more source