gRNA-SeqRET: a universal tool for targeted and genome-scale gRNA design and sequence extraction for prokaryotes and eukaryotes [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 High-throughput genetic screening is frequently employed to rapidly associate gene with phenotype and establish sequence-function relationships. With the advent of CRISPR technology, and the ability to functionally interrogate previously genetically ...
Jan-Fang Cheng
exaly +8 more sources
Deep sampling of gRNA in the human genome and deep-learning-informed prediction of gRNA activities
Cell Discovery, 2023 Life science studies involving clustered regularly interspaced short palindromic repeat (CRISPR) editing generally apply the best-performing guide RNA (gRNA) for a gene of interest.
Heng Zhang +8 more
doaj +4 more sources
BEstimate: a computational tool for the design and interpretation of CRISPR base editing experiments [PDF]
Genome BiologyCRISPR base editors enable scalable targeted DNA mutagenesis and are a powerful tool for analysing the function of variants of uncertain significance and disease modelling.
Cansu Dinçer +3 more
doaj +2 more sources
PCR-mediated One-day Synthesis of Guide RNA for the CRISPR/Cas9 System
Bio-Protocol, 2021 Nowadays, CRISPR (clustered regularly interspaced short palindromic repeats) and the CRISPR-associated protein (Cas9) system play a major role in genome editing. To target the desired sequence of the genome successfully, guide RNA (gRNA) is indispensable
Naim Hassan +3 more
doaj +1 more source
Frontiers in Cellular and Infection Microbiology, 2021 Trypanosoma cruzi, as other kinetoplastids, has a complex mechanism of editing of mitochondrial mRNAs that requires guide RNAs (gRNAs) coded in DNA minicircles in the kinetoplast. There are many variations on this mechanism among species.
Fanny Rusman +3 more
doaj +1 more source
C-SVR Crispr: Prediction of CRISPR/Cas12 guideRNA activity using deep learning models
Alexandria Engineering Journal, 2021 Clustered regularly interspaced short palindromic repeat (CRISPR) technology is the most important tool in gene editing, it can be used to target any gene using guide RNA and Cas enzyme, one limitation of CRISPR systems is low guide RNA (gRNA) activity ...
Zubaida Sa'id Ameen +4 more
doaj +1 more source
Creating a tobacco line with a weaker antifeedant property against colorado potato beetle
Биотехнология и селекция растений, 2020 Background. Genetic modification of plants is one of the promising strategies to increase their resistance to insect pests. The development of metabolic or RNA interference systems for plant protection requires appropriate models of host-insect ...
N. E. Kostina +10 more
doaj +1 more source
mBio, 2022 HIV-1 integrase (IN) has a noncatalytic function in virion maturation through its binding to the viral RNA genome (gRNA). Class II IN substitutions inhibit IN-gRNA binding and result in the formation of virions with aberrant morphologies marked by ...
Christian Shema Mugisha +8 more
doaj +1 more source
Granulin knock out zebrafish lack frontotemporal lobar degeneration and neuronal ceroid lipofuscinosis pathology. [PDF]
PLoS ONE, 2015 Loss of function mutations in granulin (GRN) are linked to two distinct neurological disorders, frontotemporal lobar degeneration (FTLD) and neuronal ceroid lipofuscinosis (NCL).
Barbara Solchenberger +4 more
doaj +1 more source
Tissue Culture Optimization and Construction of a Suitable Vector for Engineering Very Long-Chain Fatty Acids in Camelina sativa L. [PDF]
Agrotechniques in Industrial CropsOil quality from oilseeds is determined by the fatty acid (FA) content. Fatty Acid Elongase 1 (FAE1) is an essential FA gene that has been genetically modified to change the constitution of fatty acids in oilseed plants. In this study, recombinant vector
Hamideh Baghi +3 more
doaj +1 more source