Results 21 to 30 of about 44,236 (298)
In Planta Processing of the SpCas9–gRNA Complex [PDF]
Plant and Cell Physiology, 2017 In CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9)-mediated genome editing in plants, Streptococcus pyogenes Cas9 (SpCas9) protein and the required guide RNA (gRNA) are, in most cases, expressed from a stably integrated transgene.
Mikami, Masafumi +2 more
openaire +2 more sources
Screening of hFOB transfected with the pSpCas9(BB)-2A-GFP and pSpCas9(BB)-2A-GFP-gRNA-2 vectors.
, 2021 (A) Screening by GFP fluorescent microscopy analysis. Representative image of hFOB transfected with the pSpCas9(BB)-2A-GFP vector. Bright field (upper panel) and fluorescent (lower panel) images are shown.
Francesca Tonelli (11495396) +6 more
core +1 more source
Mutations identified in gRNA-2 and gRNA-3.2 transfected clones.
, 2021 Mutations identified in gRNA-2 and gRNA-3.2 transfected clones.
Francesca Tonelli (11495396) +6 more
core +1 more source
, 2023 A. The protein products of Cer1 are diagrammed at top; the GAG polyprotein contains a nucleocapsid domain (NC) and a capsid-like (CA-like) domain defined in this study.
Craig C. Mello (16469188) +3 more
core +1 more source
, 2022 (A) A schematic showing the structure of ptgs2 genes and the target sites for gRNA binding. (B) Wild-type larvae were injected with gRNAs targeting both ptgs2a and ptgs2b or gfp control.
Mengyao Niu (12295356) +3 more
core +1 more source
Frontiers in Plant Science, 2023 CRISPR/Cas9-based genome editing system is a powerful tool for plant genetic improvement. However, the variable efficiency of guide RNA(s) (gRNA) represents a key limiting factor that hampers the broad application of the CRISPR/Cas9 system in crop ...
Zhibo Wang +6 more
doaj +1 more source
Cer1 gRNA persistence after RNAi.
, 2023 For each experiment in this figure, worms were grown on empty-vector (control) bacteria until the stage indicated in brackets (see Fig 1A for staging).
Craig C. Mello (16469188) +3 more
core +1 more source
Unbiased and Tailored CRISPR/Cas gRNA Libraries by Synthesizing Covalently-closed-circular (3Cs) DNA
Bio-Protocol, 2020 Simplicity, efficiency and versatility of the CRISPR/Cas system greatly contributed to its rapid use in a broad range of fields. Applications of unbiased CRISPR/Cas screenings are increasing and thus there is a growing need for unbiased and tailored ...
Martin Wegner +2 more
doaj +1 more source
PLoS Genetics, 2023 Retroviruses and closely related LTR retrotransposons export full-length, unspliced genomic RNA (gRNA) for packaging into virions and to serve as the mRNA encoding GAG and POL polyproteins.
Bing Sun +3 more
doaj +1 more source
Sequencing of a pCas-UL39 gRNA expression vector. Chromatograms corresponding to cloned UL39 gRNA.
, 2023 Sequencing of a pCas-UL39 gRNA expression vector. Chromatograms corresponding to cloned UL39 gRNA.
Hosein Shahsavarani (12457063) +5 more
core +1 more source