C-SVR Crispr: Prediction of CRISPR/Cas12 guideRNA activity using deep learning models
Alexandria Engineering Journal, 2021 Clustered regularly interspaced short palindromic repeat (CRISPR) technology is the most important tool in gene editing, it can be used to target any gene using guide RNA and Cas enzyme, one limitation of CRISPR systems is low guide RNA (gRNA) activity ...
Zubaida Sa'id Ameen +4 more
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Mutations identified in gRNA-2 and gRNA-3.2 transfected clones.
, 2021 Mutations identified in gRNA-2 and gRNA-3.2 transfected clones.
Francesca Tonelli (11495396) +6 more
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Screening of hFOB transfected with the pSpCas9(BB)-2A-GFP and pSpCas9(BB)-2A-GFP-gRNA-2 vectors.
, 2021 (A) Screening by GFP fluorescent microscopy analysis. Representative image of hFOB transfected with the pSpCas9(BB)-2A-GFP vector. Bright field (upper panel) and fluorescent (lower panel) images are shown.
Francesca Tonelli (11495396) +6 more
core +1 more source
mBio, 2022 HIV-1 integrase (IN) has a noncatalytic function in virion maturation through its binding to the viral RNA genome (gRNA). Class II IN substitutions inhibit IN-gRNA binding and result in the formation of virions with aberrant morphologies marked by ...
Christian Shema Mugisha +8 more
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In Planta Processing of the SpCas9–gRNA Complex [PDF]
Plant and Cell Physiology, 2017 In CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9)-mediated genome editing in plants, Streptococcus pyogenes Cas9 (SpCas9) protein and the required guide RNA (gRNA) are, in most cases, expressed from a stably integrated transgene.
Mikami, Masafumi +2 more
openaire +2 more sources
Sequencing of a pCas-UL39 gRNA expression vector. Chromatograms corresponding to cloned UL39 gRNA.
, 2023 Sequencing of a pCas-UL39 gRNA expression vector. Chromatograms corresponding to cloned UL39 gRNA.
Hosein Shahsavarani (12457063) +5 more
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Tissue Culture Optimization and Construction of a Suitable Vector for Engineering Very Long-Chain Fatty Acids in Camelina sativa L. [PDF]
Agrotechniques in Industrial CropsOil quality from oilseeds is determined by the fatty acid (FA) content. Fatty Acid Elongase 1 (FAE1) is an essential FA gene that has been genetically modified to change the constitution of fatty acids in oilseed plants. In this study, recombinant vector
Hamideh Baghi +3 more
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Granulin knock out zebrafish lack frontotemporal lobar degeneration and neuronal ceroid lipofuscinosis pathology. [PDF]
PLoS ONE, 2015 Loss of function mutations in granulin (GRN) are linked to two distinct neurological disorders, frontotemporal lobar degeneration (FTLD) and neuronal ceroid lipofuscinosis (NCL).
Barbara Solchenberger +4 more
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Unbiased and Tailored CRISPR/Cas gRNA Libraries by Synthesizing Covalently-closed-circular (3Cs) DNA
Bio-Protocol, 2020 Simplicity, efficiency and versatility of the CRISPR/Cas system greatly contributed to its rapid use in a broad range of fields. Applications of unbiased CRISPR/Cas screenings are increasing and thus there is a growing need for unbiased and tailored ...
Martin Wegner +2 more
doaj +1 more source
Frontiers in Plant Science, 2023 CRISPR/Cas9-based genome editing system is a powerful tool for plant genetic improvement. However, the variable efficiency of guide RNA(s) (gRNA) represents a key limiting factor that hampers the broad application of the CRISPR/Cas9 system in crop ...
Zhibo Wang +6 more
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