Results 11 to 20 of about 44,236 (298)
Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging
Genome Biology, 2018 CRISPR/dCas9 binds precisely to defined genomic sequences through targeting of guide RNA (gRNA) sequences. In vivo imaging of genomic loci can be achieved by recruiting fluorescent proteins using either dCas9 or gRNA.
Yu Hong +4 more
doaj +2 more sources
CRISPRon/off: CRISPR/Cas9 on- and off-target gRNA design
Bioinformatics, 2022 Abstract Summary The effectiveness of CRISPR/Cas9-mediated genome editing experiments largely depends on the guide RNA (gRNA) used by the CRISPR/Cas9 system for target recognition and cleavage activation.
Christian Anthon +2 more
openaire +6 more sources
One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR) [PDF]
PLOS ONE, 2018 Novel applications based on the bacterial CRISPR system make genetic, genomic, transcriptional and epigenomic engineering widely accessible for the first time. A significant advantage of CRISPR over previous methods is its tremendous adaptability due to its bipartite nature. Cas9 or its engineered variants define the molecular effect, while short gRNAs
Christopher T. Breunig +8 more
openaire +8 more sources
Determinants of crispr nuclease grna function [PDF]
, 2023 CRISPR (clustered regularly interspaced palindromic repeats) systems are sought-after genome editing tools for therapeutics and diagnostics. The use of CRISPR systems for gene editing is not without difficulties: the specificity of Cas12a nuclease, a Cas9 alternative, presents a limit in its use for gene editing due to its inability to successfully ...
Adelakin, Adegunle Daniel,
openaire +2 more sources
Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity [PDF]
Nature Communications, 2021 Abstract CRISPR-Cas9 is a powerful DNA editing tool. A gRNA directs Cas9 to cleave any DNA sequence with a PAM. However, some gRNA sequences mediate cleavage at higher efficiencies than others. To understand this, numerous studies have screened large gRNA libraries and developed algorithms to predict gRNA sequence dependent activity ...
E. A. Moreb, M. D. Lynch
openaire +3 more sources
iScienceEradication of HIV is challenging because of the integration of proviral DNA in reservoir cells. In this study, we evaluated the antiviral effects of synthetic gRNA/Cas12a and gRNA/Cas9 ribonucleoproteins (RNPs) in HIV-infected T cells and demonstrated ...
Puja Banik +14 more
openaire +2 more sources
Structure-based design of gRNA for Cas13 [PDF]
Scientific Reports, 2020 AbstractCas13 endonuclease activity depends on the RNA local secondary structure with strong preference for single-stranded (SS) regions. Hence, it becomes indispensable to identify the SS regions for effective Cas13 mediated RNA knockdown. We herein present rational gRNA design by integrating experimental structure-seq data and predicted structural ...
Bandaru, Srinivas +9 more
openaire +2 more sources
Compact 9dBEs Enable Efficient and Precise Genome Editing in Mammalian Cells and In Vivo. [PDF]
Adv Sci (Weinh)As a compact type II‐D system, the Cas9d‐based platform holds great potential for in vivo applications. Through rational engineering, its derived base editors (9dBEs) enable efficient disease modeling while facilitating single‐vector AAV delivery for in vivo genome editing. These miniature tools offer a robust strategy for basic research and biomedical
Xiao Q +12 more
europepmc +2 more sources
Generation of the CTL4-gRNA-expressing transgenic line.
, 2022 The generation of the CTL4-gRNA-expressing transgenic line is outlined. (DOCX)
George Dimopoulos (17514) +3 more
core +1 more source
gRNA-target-sites for CCSAS paper (Zhong et al., 2021 Microbiome)
, 2023 This dataset contains CRISPR-Cas9 compatible and taxon-specific gRNA-target-site sequences, specifically designed to target host 18S rRNA genes, without affecting microeukaryotes.
Kevin Zhong (16470363)
core +1 more source