Results 11 to 20 of about 46,126 (304)
gRNA-SeqRET: A Universal Tool for Targeted and Genome-Scale gRNA Design and Sequence Extraction for Prokaryotes and Eukaryotes [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 High-throughput genetic screening is frequently employed to rapidly associate gene with phenotype and establish sequence-function relationships. With the advent of CRISPR technology, and the ability to functionally interrogate previously genetically ...
Jan-Fang Cheng
exaly +8 more sources
Deep sampling of gRNA in the human genome and deep-learning-informed prediction of gRNA activities
Cell Discovery, 2023 Life science studies involving clustered regularly interspaced short palindromic repeat (CRISPR) editing generally apply the best-performing guide RNA (gRNA) for a gene of interest.
Heng Zhang +8 more
doaj +4 more sources
Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging
Genome Biology, 2018 CRISPR/dCas9 binds precisely to defined genomic sequences through targeting of guide RNA (gRNA) sequences. In vivo imaging of genomic loci can be achieved by recruiting fluorescent proteins using either dCas9 or gRNA.
Yu Hong +4 more
doaj +2 more sources
CRISPR-Cas9 Dual-gRNA Attack Causes Mutation, Excision and Inversion of the HIV-1 Proviral DNA
Viruses, 2020 Although several studies demonstrated that the HIV proviral DNA can be effectively targeted and inactivated by the CRISPR-Cas9 system, the precise inactivation mechanism has not yet been analyzed.
Caroline S. Binda +3 more
doaj +2 more sources
Jurnal Hama dan Penyakit Tumbuhan Tropika, 2023 The xa13 gene is a recessive resistance gene against Xanthomonas oryzae pv. oryzae (Xoo) found in several rice varieties. Activation of this gene will trigger the formation of sucrose as a nutrient supply to Xoo for their growth in the plant.
Wulan Arum Hardiyani +3 more
doaj +1 more source
PCR-mediated One-day Synthesis of Guide RNA for the CRISPR/Cas9 System
Bio-Protocol, 2021 Nowadays, CRISPR (clustered regularly interspaced short palindromic repeats) and the CRISPR-associated protein (Cas9) system play a major role in genome editing. To target the desired sequence of the genome successfully, guide RNA (gRNA) is indispensable
Naim Hassan +3 more
doaj +1 more source
Generation of the CTL4-gRNA-expressing transgenic line.
, 2022 The generation of the CTL4-gRNA-expressing transgenic line is outlined. (DOCX)
George Dimopoulos (17514) +3 more
core +2 more sources
Structure-based design of gRNA for Cas13 [PDF]
Scientific Reports, 2020 AbstractCas13 endonuclease activity depends on the RNA local secondary structure with strong preference for single-stranded (SS) regions. Hence, it becomes indispensable to identify the SS regions for effective Cas13 mediated RNA knockdown. We herein present rational gRNA design by integrating experimental structure-seq data and predicted structural ...
Bandaru, Srinivas +9 more
openaire +2 more sources
Frontiers in Cellular and Infection Microbiology, 2021 Trypanosoma cruzi, as other kinetoplastids, has a complex mechanism of editing of mitochondrial mRNAs that requires guide RNAs (gRNAs) coded in DNA minicircles in the kinetoplast. There are many variations on this mechanism among species.
Fanny Rusman +3 more
doaj +1 more source
Creating a tobacco line with a weaker antifeedant property against colorado potato beetle
Биотехнология и селекция растений, 2020 Background. Genetic modification of plants is one of the promising strategies to increase their resistance to insect pests. The development of metabolic or RNA interference systems for plant protection requires appropriate models of host-insect ...
N. E. Kostina +10 more
doaj +1 more source