Results 31 to 40 of about 44,236 (298)
, 2020 The graphs on the left analyzed indel frequencies within ±10 bp from theoretical gRNA breakpoints. The graphs on the right depicted PCR sequence aberrations; theoretical gRNA cuts were indicated by blue lines. A1 & A2. The RhoA gRNA5 region in AGS cells.
Jaffer A. Ajani (8447382) +10 more
core +1 more source
Development of Target-Specific gRNAs for CRISPR-Cas9 Mediated Antifungal Resistance in Sweet Pepper [PDF]
BIO Web of ConferencesUsing CRISPR-Cas9, the gRNA for antifungal resistance to mildew diseases in Pepper species, especially Capsicum annuum, is designed. The Downey Mildew Resistance (DMR), and Enhanced Disease Resistance (EDR) genes were chosen, and the target-specific gRNA
M Sedarshini +5 more
doaj +1 more source
The insect-phase gRNA transcriptome in Trypanosoma brucei [PDF]
Nucleic Acids Research, 2013 One of the most striking examples of small RNA regulation of gene expression is the process of RNA editing in the mitochondria of trypanosomes. In these parasites, RNA editing involves extensive uridylate insertions and deletions within most of the mitochondrial messenger RNAs (mRNAs).
Koslowsky, Donna +4 more
openaire +2 more sources
A multiplex guide RNA expression system and its efficacy for plant genome engineering
Plant Methods, 2020 Background The Streptococcus pyogenes CRISPR system is composed of a Cas9 endonuclease (SpCas9) and a single-stranded guide RNA (gRNA) harboring a target-specific sequence.
Youngbin Oh +3 more
doaj +1 more source
Vavilov Journal of Genetics and Breeding, 2020 It has long been known that defects in the structure of the mitochondrial genome can cause various neuromuscular and neurodegenerative diseases. Nevertheless, at present there is no effective method for treating mitochondrial diseases. The major problem with the treatment of such diseases is associated with mitochondrial DNA (mtDNA) heteroplasmy.
E. G. Zakirova +5 more
openaire +4 more sources
A Meta-Analysis of gRNA Library Screens Enables an Improved Understanding of the Impact of gRNA Folding and Structural Stability on CRISPR-Cas9 Activity [PDF]
The CRISPR Journal, 2021 CRISPR systems are known to be inhibited by unwanted secondary structures that form within the guide RNA (gRNA). The minimum free energy of predicted secondary structures has been used in prediction algorithms. However, the types of structures as well as the degree to which a predicted structure can inhibit Cas9/gRNA activity is not well characterized.
E.A. Moreb, Michael D. Lynch
openaire +2 more sources
Targeted Gene Mutation in Rice Using a CRISPR-Cas9 System
Bio-Protocol, 2014 RNA-guided genome editing (RGE) using bacterial type II cluster regularly interspaced short palindromic repeats (CRISPR)–associated nuclease (Cas) has emerged as a simple and versatile tool for genome editing in many organisms including plant and crop ...
Kabin Xie +2 more
doaj +1 more source
Scientific Reports, 2021 Rapid and accurate species diagnosis accelerates performance in numerous biological fields and associated areas. However, morphology-based species taxonomy/identification might hinder study and lead to ambiguous results.
Kittisak Buddhachat +5 more
doaj +1 more source
Augmenting and Assaying Nav1.1 Protein Quantity for Dravet Syndrome Therapy. [PDF]
Ann Clin Transl NeurolABSTRACT Dravet Syndrome (DS) is a developmental and epileptic encephalopathy predominantly caused by heterozygous loss‐of‐function variants in SCN1A, which encodes Nav1.1. Conserved upstream open reading frames (uORFs) in SCN1A were validated to regulate translation in reporter assays, demonstrating the therapeutic viability of increasing Nav1.1 from ...
Saravanan A +7 more
europepmc +2 more sources
CERV structure and function in gRNA export.
, 2023 A. The left image is the AlphaFold-predicted structural model for the complete CERV protein (aa1-517), with the pLDDT color-coded confidence scores as in Fig 2A.
Craig C. Mello (16469188) +3 more
core +1 more source