Results 31 to 40 of about 46,126 (304)
PLoS Genetics, 2023 Retroviruses and closely related LTR retrotransposons export full-length, unspliced genomic RNA (gRNA) for packaging into virions and to serve as the mRNA encoding GAG and POL polyproteins.
Bing Sun +3 more
doaj +1 more source
Development of Target-Specific gRNAs for CRISPR-Cas9 Mediated Antifungal Resistance in Sweet Pepper [PDF]
BIO Web of ConferencesUsing CRISPR-Cas9, the gRNA for antifungal resistance to mildew diseases in Pepper species, especially Capsicum annuum, is designed. The Downey Mildew Resistance (DMR), and Enhanced Disease Resistance (EDR) genes were chosen, and the target-specific gRNA
M Sedarshini +5 more
doaj +1 more source
A Meta-Analysis of gRNA Library Screens Enables an Improved Understanding of the Impact of gRNA Folding and Structural Stability on CRISPR-Cas9 Activity [PDF]
The CRISPR Journal, 2021 CRISPR systems are known to be inhibited by unwanted secondary structures that form within the guide RNA (gRNA). The minimum free energy of predicted secondary structures has been used in prediction algorithms. However, the types of structures as well as the degree to which a predicted structure can inhibit Cas9/gRNA activity is not well characterized.
E.A. Moreb, Michael D. Lynch
openaire +2 more sources
, 2020 The graphs on the left analyzed indel frequencies within ±10 bp from theoretical gRNA breakpoints. The graphs on the right depicted PCR sequence aberrations; theoretical gRNA cuts were indicated by blue lines. A1 & A2. The RhoA gRNA5 region in AGS cells.
Jaffer A. Ajani (8447382) +10 more
core +1 more source
Selected piezo1 gRNA creates indels in target sequence.
, 2023 (A) Sanger sequencing of 3 individual animals following injection with a scrambled gRNA along with Cas9 (Top). Sanger sequencing of 3 individual animals following injection with a piezo1 gRNA along with Cas9 (Bottom).
Cody J. Smith (6630881) +1 more
core +1 more source
Cer1 gRNA persistence after RNAi.
, 2023 For each experiment in this figure, worms were grown on empty-vector (control) bacteria until the stage indicated in brackets (see Fig 1A for staging).
Craig C. Mello (16469188) +3 more
core +1 more source
Targeted Gene Mutation in Rice Using a CRISPR-Cas9 System
Bio-Protocol, 2014 RNA-guided genome editing (RGE) using bacterial type II cluster regularly interspaced short palindromic repeats (CRISPR)–associated nuclease (Cas) has emerged as a simple and versatile tool for genome editing in many organisms including plant and crop ...
Kabin Xie +2 more
doaj +1 more source
A multiplex guide RNA expression system and its efficacy for plant genome engineering
Plant Methods, 2020 Background The Streptococcus pyogenes CRISPR system is composed of a Cas9 endonuclease (SpCas9) and a single-stranded guide RNA (gRNA) harboring a target-specific sequence.
Youngbin Oh +3 more
doaj +1 more source
A mRNA determinant of gRNA-directed kinetoplastid editing [PDF]
Nucleic Acids Research, 2001 Several mitochondrial mRNAs of the kinetoplastid protozoa do not encode a functional open reading frame until they have been edited through the addition or deletion of U nucleotides at specific sites. Genetic information specifying the location and extent of editing is present on guide RNAs (gRNAs).
A L, Kabb +3 more
openaire +2 more sources
The insect-phase gRNA transcriptome in Trypanosoma brucei [PDF]
Nucleic Acids Research, 2013 One of the most striking examples of small RNA regulation of gene expression is the process of RNA editing in the mitochondria of trypanosomes. In these parasites, RNA editing involves extensive uridylate insertions and deletions within most of the mitochondrial messenger RNAs (mRNAs).
Koslowsky, Donna +4 more
openaire +2 more sources