Results 51 to 60 of about 44,236 (298)
S100A8 gRNA sequences in CRISPR/Cas9 vector.
, 2021 S100A8 gRNA sequences in CRISPR/Cas9 vector.
Lei Li (29537) +7 more
core +1 more source
Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR-Cas12a gRNA switch. [PDF]
, 2021 CRISPR technologies increasingly require spatiotemporal and dosage control of nuclease activity. One promising strategy involves linking nuclease activity to a cell's transcriptional state by engineering guide RNAs (gRNAs) to function only after ...
Beisel, Chase L +9 more
core +1 more source
Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining
Molecular Oncology, EarlyView.IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis +3 more
wiley +1 more source
Frontiers in Plant Science, 2020 Alfalfa (Medicago sativa) is an outcrossing tetraploid legume species widely cultivated in the world. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system has been successfully used for ...
Tezera W. Wolabu +14 more
doaj +1 more source
Viruses, 2022 Retroviruses must selectively recognize their unspliced RNA genome (gRNA) among abundant cellular and spliced viral RNAs to assemble into newly formed viral particles.
Serena Bernacchi
doaj +1 more source
Finding novel vulnerabilities of hypomorphic BRCA1 alleles
Molecular Oncology, EarlyView.Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder +10 more
wiley +1 more source
Nghiên cứu tạo phôi chuột knockout gene lep sử dụng kỹ thuật CRISPR/Cas9
Tạp chí Khoa học Đại học Cần Thơ, 2022 Kỹ thuật CRISPR/Cas 9 đang được sử dụng nhiều trong công nghệ chỉnh sửa gene. Trong nghiên cứu này, phôi chuột knockout gene Lep được tạo ra bằng cách vi tiêm plasmid có cấu trúc CRISPR/Cas9 hướng đến exon 3 của gene.
Âu Dương Tuyết Mai +6 more
doaj +1 more source
Interactions of mRNAs and gRNAs involved in trypanosome mitochondrial RNA editing: Structure probing of a gRNA bound to its cognate mRNA [PDF]
RNA, 2006 Expression of mitochondrial genes in Trypanosoma brucei requires RNA editing of its mRNA transcripts. During editing, uridylates are precisely inserted and deleted as directed by the gRNA template to create the protein open reading frame. This process involves the bimolecular interaction of the gRNA with its cognate pre-edited mRNA and the assembly of ...
Laura E, Yu, Donna J, Koslowsky
openaire +2 more sources
Sheet 1: computational output showing gRNA properties.
, 2021 Sheet 2: gRNA oligonucleotide sequences ordered for cloning into pCFD3.1 vector. (XLSX)
Enas Gad El-Karim (11632158) +3 more
core +1 more source