Results 51 to 60 of about 46,126 (304)
, 2022 (A) A schematic showing the structure of ptgs2 genes and the target sites for gRNA binding. (B) Wild-type larvae were injected with gRNAs targeting both ptgs2a and ptgs2b or gfp control.
Mengyao Niu (12295356) +3 more
core +1 more source
List of gRNA cassettes used in this study for multiplex gene activation by CRISPR-dCas9.
, 2021 Sequences of gRNA cassette fragment (HH–20 bp specific determinant sequences–structural gRNA–HDV) with the addition of EcoRI and KpnI restriction sites for pOtAOX-gRNA plasmids construction in this study. EcoRI (gaattc) and KpnI (ggtacc) sequences are in
Sutipa Tanapongpipat (3126720) +3 more
core +1 more source
On the Selective Packaging of Genomic RNA by HIV-1
Viruses, 2016 Like other retroviruses, human immunodeficiency virus type 1 (HIV-1) selectively packages genomic RNA (gRNA) during virus assembly. However, in the absence of the gRNA, cellular messenger RNAs (mRNAs) are packaged.
Mauricio Comas-Garcia +2 more
doaj +1 more source
, 2023 A. The protein products of Cer1 are diagrammed at top; the GAG polyprotein contains a nucleocapsid domain (NC) and a capsid-like (CA-like) domain defined in this study.
Craig C. Mello (16469188) +3 more
core +1 more source
Molecular Oncology, EarlyView.This study identifies ARHGAP5, in addition to the frequently mutated ARHGAP35, as significantly mutated in endometrial cancer. Mutations in both genes co‐occur and are associated with their correlated downregulation. Functional CRISPR studies show that both paralogs regulate similar pathways, including actin cytoskeleton organization.
Mathilde Pinault +12 more
wiley +1 more source
Structure and use of ribozyme to generate gRNA.
, 2016 (A) Sequence of HH ribozyme. Map of HH-gRNA. First 6 bases (grey text) of the ribozyme are complementary to the first six bases of the gRNA (red text) and cleavage occurs at the 5’ end of the gRNA (arrow). (B) Denaturing PAGE gel of IVT RNA.
Ashley Shu Mei Ng (493205) +2 more
core +1 more source
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
iScienceEradication of HIV is challenging because of the integration of proviral DNA in reservoir cells. In this study, we evaluated the antiviral effects of synthetic gRNA/Cas12a and gRNA/Cas9 ribonucleoproteins (RNPs) in HIV-infected T cells and demonstrated their specificity and efficacy in disrupting HIV gene replication and expression. Notably, sequential
Puja Banik +14 more
openaire +4 more sources
Frontiers in Plant Science, 2020 Alfalfa (Medicago sativa) is an outcrossing tetraploid legume species widely cultivated in the world. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system has been successfully used for ...
Tezera W. Wolabu +14 more
doaj +1 more source