Results 71 to 80 of about 46,126 (304)

A Customizable Protocol for String Assembly gRNA Cloning (STAgR) [PDF]

Journal of Visualized Experiments, 2018
The bacterial CRISPR/Cas9 system has substantially increased methodological options for life scientists. Due to its utilization, genetic and genomic engineering became applicable to a large range of systems. Moreover, many transcriptional and epigenomic engineering approaches are now generally feasible for the first time.
Breunig, C., Neuner, A.M., Giehrl-Schwab, J., Wurst, W., Götz, M., Stricker, S.H.   +5 more
openaire   +3 more sources

KDAC6 alters cell morphology and motility through positive and negative modulation of F‐actin distribution

FEBS Open Bio, EarlyView.
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph, Andrea B. Pham, Kiara E. Bornes, Razan H. Hammad, Chelsea A. Rousseve‐Ross, Thomas M. Huckaba, Terry J. Watt, Tasha B. Toro   +7 more
wiley   +1 more source

Comprehensive approaches to design efficient gRNA for SDN1-CRISPR/Cas9 genome editing in wheat

Frontiers in Genome Editing
BackgroundCRISPR/Cas9 technology has gained popularity due to its efficient, widely applicable, and relatively easy genome editing. Furthermore, the removal of regulation on site-directed nuclease1- (SDN1) and SDN2-developed products in many countries ...
Tushadri Singh, Tushadri Singh, H. M. Mamrutha, Rajender Singh, J. P. Jaiswal, Zeenat Wadhwa, Rakesh Kumar, Omvir Singh, O. P. Ahlawat, Ratan Tiwari   +9 more
doaj   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Retroviral RNA Dimerization: From Structure to Functions

Frontiers in Microbiology, 2018
The genome of the retroviruses is a dimer composed by two homologous copies of genomic RNA (gRNA) molecules of positive polarity. The dimerization process allows two gRNA molecules to be non-covalently linked together through intermolecular base-pairing.
Noé Dubois, Roland Marquet, Jean-Christophe Paillart, Serena Bernacchi   +3 more
doaj   +1 more source

Intronic gRNAs for the Construction of Minimal Gene Drive Systems

Frontiers in Bioengineering and Biotechnology, 2022
Gene drives are promising tools for the genetic control of insect vector or pest populations. CRISPR-based gene drives are generally highly complex synthetic constructs consisting of multiple transgenes and their respective regulatory elements. This complicates the generation of new gene drives and the testing of the behavior of their constituent ...
Nash, A, Paolo, C, Hoermann, A, Papathanos, P, Windbichler, N   +4 more
openaire   +4 more sources

Augmenting and Assaying Nav1.1 Protein Quantity for Dravet Syndrome Therapy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Dravet Syndrome (DS) is a developmental and epileptic encephalopathy predominantly caused by heterozygous loss‐of‐function variants in SCN1A, which encodes Nav1.1. Conserved upstream open reading frames (uORFs) in SCN1A were validated to regulate translation in reporter assays, demonstrating the therapeutic viability of increasing Nav1.1 from ...
Aiswarya Saravanan, Jordan Safran, Anna Loughman, Grace Lin, Shivanshi Vaid, Dana Allababidi, Peter M. Tessier, Louis T. Dang   +7 more
wiley   +1 more source

Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering

eLife, 2017
Chemical modification of the gRNA and donor DNA has great potential for improving the gene editing efficiency of Cas9 and Cpf1, but has not been investigated extensively.
Kunwoo Lee, Vanessa A Mackley, Anirudh Rao, Anthony T Chong, Mark A Dewitt, Jacob E Corn, Niren Murthy   +6 more
doaj   +1 more source

Cell Compartment-Specific Folding of Ty1 Long Terminal Repeat Retrotransposon RNA Genome

Viruses, 2022
The structural transitions RNAs undergo during trafficking are not well understood. Here, we used the well-developed yeast Ty1 retrotransposon to provide the first structural model of genome (g) RNA in the nucleus from a retrovirus-like transposon ...
Małgorzata Zawadzka, Angelika Andrzejewska-Romanowska, Julita Gumna, David J. Garfinkel, Katarzyna Pachulska-Wieczorek   +4 more
doaj   +1 more source

CRISPRon/off: CRISPR/Cas9 on- and off-target gRNA design

Bioinformatics, 2022
Abstract Summary The effectiveness of CRISPR/Cas9-mediated genome editing experiments largely depends on the guide RNA (gRNA) used by the CRISPR/Cas9 system for target recognition and cleavage activation.
Christian Anthon, Giulia Ilaria Corsi, Jan Gorodkin   +2 more
openaire   +5 more sources