Results 91 to 100 of about 44,236 (298)

Divergent susceptibilities to AAV-SaCas9-gRNA vector-mediated genome-editing in a single-cell-derived cell population

BMC Research Notes, 2017
Objective Recombinant adeno-associated virus (AAV)-based vectors are characterized by their robust and safe transgene delivery. The CRISPR/Cas9 and guide RNA (gRNA) system present a promising genome-editing platform, and a recent development of a shorter
Salma G. Morsy, Jason M. Tonne, Yaxi Zhu, Brian Lu, Karol Budzik, James W. Krempski, Sherine A. Ali, Mohamed A. El-Feky, Yasuhiro Ikeda   +8 more
doaj   +1 more source

SiDT1 Defines Plant Architecture Reminiscent of Green Revolution in Foxtail Millet

Advanced Science, EarlyView.
SiDT1 encodes a GA3‐oxidase that creates a semi‐dwarf, lodging‐resistant architecture reminiscent of the rice Green Revolution. The resulting ideotype performs well under dense planting and provides a valuable genetic resource for high‐yield, mechanized foxtail millet production. ABSTRACT Foxtail millet (Setaria italica) is a drought‐tolerant C4 cereal
Jianzhen Lv, Jinjin Cheng, Zhen Hu, Qian Lan, Yanjun Yang, Chengcheng Pei, Junhao Luo, Zhuang Li, Peiyong Xin, Jijun Yan, Jinfang Chu, Qian Qian, Zhaosheng Kong, Liang Jiang   +13 more
wiley   +1 more source

MiR-145 mediates zebrafish hepatic outgrowth through progranulin A signaling.

PLoS ONE, 2017
MicroRNAs (miRs) are mRNA-regulatory molecules that fine-tune gene expression and modulate both processes of development and tumorigenesis. Our previous studies identified progranulin A (GrnA) as a growth factor which induces zebrafish hepatic outgrowth ...
Ya-Wen Li, Keng-Yu Chiang, Yen-Hsing Li, Sung-Yu Wu, Wangta Liu, Chia-Ray Lin, Jen-Leih Wu   +6 more
doaj   +1 more source

The impact of mRNA structure on guide RNA targeting in kinetoplastid RNA editing.

PLoS ONE, 2010
Mitochondrial mRNA editing in Trypanosoma brucei requires the specific interaction of a guide RNA with its cognate mRNA. Hundreds of gRNAs are involved in the editing process, each needing to target their specific editing domain within the target message.
Larissa Reifur, Laura E Yu, Jorge Cruz-Reyes, Michelle Vanhartesvelt, Donna J Koslowsky   +4 more
doaj   +1 more source

mRNA/gRNA binding affinity by EMSA.

, 2013
A–D: Representative images of 6% polyacrylamide gels and corresponding binding isotherms with the apparent dissociation constants (KD). Samples contained 5 nM 32P-labeled gRNA (gA6-14, gCYb-558 and gND7-550) or 10 nM 32P-labeled gRNA (gND7-506) and ...
Donna J. Koslowsky (362427), Larissa Reifur (362422), Jorge Cruz-Reyes (12817), Laura E. Yu (362424), Michelle vanHartesvelt (362426)   +4 more
core   +1 more source

A Pan‐Methylome Framework for Population‐Scale Bacterial Epigenomics

Advanced Science, EarlyView.
A scalable quantitative framework unlocks population‐level comparative epigenomics in bacteria. By transforming site‐level data into standardized traits, this approach reconstructs methylation‐informed phylogenies and defines the core epigenome.
Bin Ma, Huimin Gong, Ran Zhuo, Zaixiao Rao, Yanxi Wan, Mingyang Wang, Zahra Zeinalzadeh, Yuan Gao, Aohan Guan, Xinrun Li, Wanting Chen, Xiliang Wang, Yun Wan, Sihua Zhang, Yuncai Xiao, Qi Huang, Paul R. Langford, Xiangru Wang, Rui Zhou, Rui Luo, Hongbo Zhou, Chengliang Zhu, Hui Jin   +22 more
wiley   +1 more source

Variation in guide RNA library representation results in gene effect score bias in genome-wide CRISPR screens

BMC Genomics
Genome wide CRISPR-based perturbation screens are powerful discovery tools enabling the identification of novel gene dependencies through either gain or loss of function. While genome wide guide RNA (gRNA) libraries have advantages when using enAsCas12a,
Paul Metz, Sofia Alves-Vasconcelos, Richard Wallbank, Joey Riepsaame, Stuart Brown, A. Bassim Hassan   +5 more
doaj   +1 more source

Generation of Grna and Grnb KOs using ZFNs.

, 2015
A: Schematic illustration of human GRN and zebrafish Granulins. Human GRN has 7 ½ granulin domains, while 12 granulin domains are found in Grna, 9 in Grnb, and 1 ½ in Grn1 and Grn2. Grey: signal peptide. Black numbers: amino acids.
Christian Haass (261040), Bettina Schmid (362817), Barbara Solchenberger (705072), Claire Russell (484748), Elisabeth Kremmer (17351)   +4 more
core   +1 more source

PEAR1 Promotes Glucose Metabolism Reprogramming in Sepsis‐Associated Acute Lung Injury via AARS1‐Mediated HIF‐1α Lactylation

Advanced Science, EarlyView.
This study revealed that a PEAR1/HIF‐1α/ glycolysis/lactate/H3K18la positive feedback loop in PMVECs that drives the development of S‐ALI. Mechanistically, PEAR1 mediates the binding of HIF‐1α to AARS1, leading to the lactylation of HIF‐1α, the primary lactylation site of which is K172.
Shuai Li, Zongqing Lu, Pu Fang, Qingqing Pang, Xianghui Wu, Bo Wang, Min Liang, Jingjing Pan, Haobo Kong, Haijian Cai, Juanjuan Li, Jiaying Kang, Dongsheng Wang, Dunling Xia, Mengmeng Xu, Qinghai You   +15 more
wiley   +1 more source

Interrogating the Escherichia coli Epitranscriptome Via CRISPR Interference and Nanopore Native RNA Sequencing. [PDF]

Microbiologyopen
Epitranscriptomics is understudied in bacteria but has the ability to modulate antimicrobial resistance, virulence and pathogenicity. Pitt et al. implement two cutting‐edge techniques: CRISPR interference and Nanopore direct RNA sequencing along with functional assays to study RNA modifications in Escherichia coli.
Pitt ME, Zhang J, Nguyen ANT, Hall MB, Jebeli L, Featherstone LA, Myers GSA, Scott NE, Coin LJM.   +8 more
europepmc   +2 more sources