Results 101 to 110 of about 44,236 (298)

Characterization of gRNA-dependent and gRNA-independent off-target binding sites of PspCas13b and RfxCas13d in mammalian cells

Nucleic Acids Research
Abstract CRISPR–Cas13 systems, harnessed for RNA-guided transcriptome editing, hold significant promise for clinical and in vivo therapeutic applications. However, understanding their in vivo target specificity and recognition rules remains a challenge.
Huanhuan Feng, Zhixue Li, Hongdao Zhang, Yuli Zheng, Beiying Xu, Yao Zhang, Lin Zou, Ligang Wu   +7 more
openaire   +2 more sources

Multiplexed gene activation using the SAM system with gRNA arrays.

, 2018
(a) Diagram depicting the elements encoded in plasmids used for multiplex gene activation using the SAM system combined with gRNA arrays containing MS2 sequences. (b) RT-PCR results of gene activation at 5 gRNA target sites 3 days post transfection using
Kai Hui (5753966), Natalie K. Wolf (5753951), Walker S. Lahr (5753954), Mitchell G. Kluesner (5753960), Samantha Lee (5753963), Morito Kurata (154677), Madison T. Weg (5753957), Masano Shiraiwa (5753969), Branden S. Moriarity (559376), Beau R. Webber (5753972)   +9 more
core   +1 more source

Microprotein MP104 Promotes Malignant Progression of Colorectal Cancer Through Regulating Protein Translation

Advanced Science, EarlyView.
A previously unrecognized microprotein, MP104, encoded by ZEB1‐AS1, emerges as a critical driver of colorectal cancer progression. MP104 links ubiquitin‐mediated protein degradation with translational reprogramming via the UBE2O–AMPKα2–mTOR–EIF4B axis, revealing an unrecognized layer of oncogenic regulation.
Fang Chen, Miao Wang, Hongmei Yong, Jin Ding, Shiping Xu, Qirui Ge, Yan Wang, Lei Zhang, Qianqian Xiao, Benli Li, Li Lin, Sufang Chu, Qianqing Wang, Jin Bai, Pingfu Hou   +14 more
wiley   +1 more source

Role of Purine-Rich Regions in Mason-Pfizer Monkey Virus (MPMV) Genomic RNA Packaging and Propagation

Frontiers in Microbiology, 2020
A distinguishing feature of the Mason-Pfizer monkey virus (MPMV) packaging signal RNA secondary structure is a single-stranded purine-rich sequence (ssPurines) in close vicinity to a palindromic stem loop (Pal SL) that functions as MPMV dimerization ...
Lizna Mohamed Ali, Fathima Nuzra Nagoor Pitchai, Valérie Vivet-Boudou, Akhil Chameettachal, Ayesha Jabeen, Vineeta N. Pillai, Farah Mustafa, Farah Mustafa, Roland Marquet, Tahir A. Rizvi, Tahir A. Rizvi   +10 more
doaj   +1 more source

Nucleotide sequence of gRNA to target site.

, 2017
Nucleotide sequence of gRNA to target site.
Chen Zhang (66790), Yanqin Ji (3778729), Jing Jin (18601), Wenhui Zhang (425538), Man Wang (110466), Yingjie Wang (354135), Qisheng Zuo (690242), Zhenyu Lu (1531282), Dong Li (212687), Fei Wang (19534), Yani Zhang (446928), Bichun Li (446931)   +11 more
core   +1 more source

gRNA target sites for lrrk2 locus deletion.

, 2021
gRNA target sites for lrrk2 locus deletion.
Volker Kroehne (672204), Stefan Hans (186256), Michael Brand (186270), Avinash Chekuru (11426773), Jan Kaslin (186253), Reiner Ahrendt (11426767), Svetlana A. Semenova (11426770), Shady Sayed (11426782), Stefano Suzzi (11426764), Anja Machate (186262), Paul Wirsching (11426776), Sylke Winkler (2541403), Pertti Panula (4695), Saygın Bilican (11426779), Sandra Spieß (11426785)   +14 more
core   +1 more source

Computation and Structure‐Guided Arginine Scanning Engineers a Hyperactive AP Endonuclease for Multiplex Viral RNA Sensing

Advanced Science, EarlyView.
Computational and structure‐guided arginine scanning rewires the DNA‐binding interface of APE1 to create APE1‐Evo, a hyperactive yet specific AP endonuclease. Integrated into the NAPTUNE‐V2.0 cascade, APE1‐Evo enables amplification‐free, multiplex viral RNA sensing for dengue virus and influenza A/B, highlighting a general strategy for engineering ...
Junlan Wang, Ting Wu, Feizuo Wang, Boyang Wang, Xin Yu Wong, Senfeng Zhang, Shengsheng Ma, Yao Liu, Fangyao Yao, Zhifeng Zeng, Siyuan Yang, Kunming Liu, Qi Wang, Yilong Wang, Yunxiang Zang, Xiaolong Wang, Xingxu Huang, Shao Li, Quanquan Ji, Tao Hu, Chunyi Hu   +20 more
wiley   +1 more source

Visualizing Association of the Retroviral Gag Protein with Unspliced Viral RNA in the Nucleus

mBio, 2020
Packaging of genomic RNA (gRNA) by retroviruses is essential for infectivity, yet the subcellular site of the initial interaction between the Gag polyprotein and gRNA remains poorly defined.
Rebecca J. Kaddis Maldonado, Breanna Rice, Eunice C. Chen, Kevin M. Tuffy, Estelle F. Chiari, Kelly M. Fahrbach, Thomas J. Hope, Leslie J. Parent   +7 more
doaj   +1 more source

Hazelnut allergome overview and Cor a gRNAs identification

BMC Plant Biology
Corylus species (hazelnuts) are a valuable source of nutrients and are widely consumed worldwide. Nevertheless, Corylus avellana (Cor a) contains 13 allergens (Cor a 1, Cor a 2, Cor a 6, Cor a 8, Cor a 9, Cor a 10, Cor a 11, Cor a 12, Cor a 13, Cor a 14, Cor a 15, Cor a 16, and Cor a TLP) that have been deposited into the official database (WHO/IUIS ...
Amoroso, Ciro Gianmaria, Andolfo, Giuseppe   +1 more
openaire   +4 more sources

Efficiency of mutation induction by each gRNA.

, 2018
(A) Schematics of the drug resistance genes and targeted region of each gRNA. Underlines represent the gRNA recognition region, the PAM sequence (blue), and the target sequence (red).
Satoshi Abe (524075), Narumi Uno (202615), Takeshi Endo (743010), Tetsuya Ohbayashi (229099), Kazuhisa Honma (4911754), Yasuhiro Kazuki (202667), Mitsuo Oshimura (8238)   +6 more
core   +1 more source