Results 101 to 110 of about 46,126 (304)
Advanced Science, EarlyView.D4F‐functionalized ceria nanozyme–CasRx nanoparticles preferentially target renal macrophages through SR‐B1‐mediated uptake in ischemia–reperfusion injury. Ceria scavenges excessive ROS, while CasRx silences STING signaling, shifting macrophages toward a less inflammatory, pro‐resolving state and increasing the proportion of regulatory T cells.
Weibo Chen +11 more
wiley +1 more source
PLOS ONE, 2018 Novel applications based on the bacterial CRISPR system make genetic, genomic, transcriptional and epigenomic engineering widely accessible for the first time. A significant advantage of CRISPR over previous methods is its tremendous adaptability due to its bipartite nature. Cas9 or its engineered variants define the molecular effect, while short gRNAs
Christopher T. Breunig +8 more
openaire +6 more sources
Expansion of mapped gRNA peak to nucleotide level.
, 2015 A mapped gRNA peak from mc71 encoding the gND9X gRNA is expanded to the nucleotide level to illustrate the characteristic 5’ flush edge and 3’ heterogeneity.
Feng Li (30515) +4 more
core +1 more source
Advanced Science, EarlyView.CDegSR, a new tool for imaging RNA at single molecule level in living cells was developed by researchers. Background noise was eliminated by protein engineering and making it degrade when not bound to its target. This allowed individual RNA molecules to be clearly imaged and tracked.
Shipeng Shao, Hongchen Zhang
wiley +1 more source
MiR-145 mediates zebrafish hepatic outgrowth through progranulin A signaling.
PLoS ONE, 2017 MicroRNAs (miRs) are mRNA-regulatory molecules that fine-tune gene expression and modulate both processes of development and tumorigenesis. Our previous studies identified progranulin A (GrnA) as a growth factor which induces zebrafish hepatic outgrowth ...
Ya-Wen Li +6 more
doaj +1 more source
Nucleic Acids ResearchAbstract CRISPR–Cas13 systems, harnessed for RNA-guided transcriptome editing, hold significant promise for clinical and in vivo therapeutic applications. However, understanding their in vivo target specificity and recognition rules remains a challenge.
Huanhuan Feng +7 more
openaire +2 more sources
Advanced Science, EarlyView.This work develops a metabolic sharing strategy that employs cytidine monophosphate (CMP) to competitively reprogram floxuridine (FUDR) pyrimidine metabolism in colorectal tumor cells. Elevated intracellular FdUMP induces nucleotide imbalance, drives mitochondrial DNA release and cGAS‐STING innate immune cascade activation, and potentiates robust ...
Chao Wang +10 more
wiley +1 more source
BMC Research Notes, 2017 Objective Recombinant adeno-associated virus (AAV)-based vectors are characterized by their robust and safe transgene delivery. The CRISPR/Cas9 and guide RNA (gRNA) system present a promising genome-editing platform, and a recent development of a shorter
Salma G. Morsy +8 more
doaj +1 more source
Advanced Science, EarlyView.A low dose of dexmedetomidine, delivered by ROS‐responsive nanoparticles, preferentially accumulates in the ischemic intestine and restores gut vascular barrier integrity after ischemia/reperfusion injury. This nanoparticle‐based strategy prevents bacterial translocation and liver damage without cardiorespiratory side effects, working through HDAC ...
Hu‐Fei Zhang +10 more
wiley +1 more source
The impact of mRNA structure on guide RNA targeting in kinetoplastid RNA editing.
PLoS ONE, 2010 Mitochondrial mRNA editing in Trypanosoma brucei requires the specific interaction of a guide RNA with its cognate mRNA. Hundreds of gRNAs are involved in the editing process, each needing to target their specific editing domain within the target message.
Larissa Reifur +4 more
doaj +1 more source