Results 111 to 120 of about 44,236 (298)

List of gRNA cassettes used in this study for simultaneous gene mutagenesis by CRISPR-Cas9.

, 2021
Sequences of gRNA cassette fragment (HH–20 bp specific determinant sequences–structural gRNA–HDV) with the addition of EcoRI and KpnI restriction sites for pOtAOX-gRNA plasmids construction in this study. EcoRI (gaattc) and KpnI (ggtacc) sequences are in
Sutipa Tanapongpipat (3126720), Aekkachai Puseenam (2902434), Niran Roongsawang (2902437), Worarat Kruasuwan (11495350)   +3 more
core   +1 more source

Ahcy Acts as an Effector of Hnf4a‐Driven Super‐Enhancer Activation to Alleviate MASLD During Intermittent Fasting

Advanced Science, EarlyView.
Our study identifies an Hnf4a‐driven super‐enhancer of hepatic Ahcy that mediates the protective effects of intermittent fasting against MASLD. Disruption of the Ahcy super‐enhancer reduces Ahcy expression and exacerbates lipid accumulation. This pathway prevents aberrant promoter hypermethylation by maintaining the SAM/SAH balance, as exemplified by ...
Huafeng Chen, Shilin Zhang, Xiaojie Deng, Wenqiang Xie, Fen Xu, Jie Shen, Hua Liang   +6 more
wiley   +1 more source

CRISPR/Cas9-Mediated Three Nucleotide Insertion Corrects a Deletion Mutation in MRP1/ABCC1 and Restores Its Proper Folding and Function

Molecular Therapy: Nucleic Acids, 2017
A three-nucleotide deletion in cystic fibrosis transmembrane conductance regulator/ATP-binding cassette transporter C7 (CFTR/ABCC7) resulting in the absence of phenylalanine at 508 leads to mis-fold of the mutated protein and causes cystic fibrosis.
Qinqin Xu, Yue-xian Hou, Xiu-bao Chang
doaj   +1 more source

Are the current gRNA ranking prediction algorithms useful for genome editing in plants?

PLoS ONE, 2020
Introducing a new trait into a crop through conventional breeding commonly takes decades, but recently developed genome sequence modification technology has the potential to accelerate this process. One of these new breeding technologies relies on an RNA-
Fatima Naim, Kylie Shand, Satomi Hayashi, Martin O'Brien, James McGree, Alexander A T Johnson, Benjamin Dugdale, Peter M Waterhouse   +7 more
doaj   +1 more source

Target sequences of gRNA used in this study.

, 2022
This spreadsheet provides the target sequences of gRNA. (XLSX)
Yumiko Saga (176901), Hajime Okada (11944869)   +1 more
core   +1 more source

A Rare De Novo Missense Mutation in IFT122 Confers a Genetic Susceptibility Factor of Idiopathic Pediatric Uveitis Via Trio‐based Whole‐Exome Sequencing

Advanced Science, EarlyView.
A rare de novo IFT122‐A773E variant is identified in idiopathic pediatric uveitis and shown to exacerbate retinal inflammation and barrier dysfunction. Mechanistically, the variant enhances IFT43 interaction, elevates calcium signaling, and activates the MEK/ERK/FRA1 axis, revealing a previously unrecognized cilia‐associated pathway that may increase ...
Qian Zhou, Jiaxing Huang, Yilin Wang, Xingran Li, Xianyang Liu, Lingyu Dai, Hongshun Li, Qingfeng Wang, Jiangyi Liu, Guannan Su, Wanyun Zhang, Yang Deng, Qingfeng Cao, Yujie Lai, Xiang Luo, Changwei Huang, Ling Chen, Shengping Hou, Peizeng Yang   +18 more
wiley   +1 more source

Expansion of mapped gRNA peak to nucleotide level.

, 2015
A mapped gRNA peak from mc71 encoding the gND9X gRNA is expanded to the nucleotide level to illustrate the characteristic 5’ flush edge and 3’ heterogeneity.
Feng Li (30515), Larry Simpson (774451), James A. Lake (774453), Stephen M. Douglass (774452), Matteo Pellegrini (2078)   +4 more
core   +1 more source

OTUD6A‐Mediated Deubiquitination of PRDX1 Protects Against Oral Ulcer by Restoring Mitochondrial Function

Advanced Science, EarlyView.
In human oral keratinocytes, OTUD6A promoted cell migration by deubiquitinating and stabilizing PRDX1. Conversely, OTUD6A deficiency reduced PRDX1 stability, triggering mitochondrial dysfunction and aggravating OU progression. ABSTRACT Oral ulcers (OU), as the most highly prevalent and recurrent oral mucosal lesion, have an unclear pathogenesis that ...
Xiaoyu Sun, Ruiwei Jia, Xingbei Pan, Rui Huang, Jiahong Chen, Ziyi Yao, Lei Yin, Jun Ma, Hongjieliang Wang, Xinyu Huang, Yilin Ma, Yifan Ping, Yuanyuan Chen, Huining Wang, Shengbin Huang, Guang Liang   +15 more
wiley   +1 more source

Targeting CXCR2 in Nociceptive Sensory Neurons Offers Novel Therapeutic Potentials for Postoperative Pain

Advanced Science, EarlyView.
Tissue incision induces CXCL5 release, which activates neuronal CXCR2 on incision‐innervating sensory neurons. Neuronal CXCR2 couples with TRPA1 to drive mechanical pain and engages p38 MAPK signaling to upregulate TRPV1 and promote heat pain. Targeted Cxcr2 knockdown relieves incisional pain while preserving CXCR2‐dependent neutrophil responses ...
Yushuang Pan, Peiyi Li, Yufeng Chen, Zishan Dong, Yuanyuan Li, Boyu Liu, Yan Tai, Chuan Wang, Jianqiao Fang, Chengyu Yin, Boyi Liu   +10 more
wiley   +1 more source

Development of Strand-Specific Real-Time RT-PCR to Distinguish Viral RNAs during Newcastle Disease Virus Infection

The Scientific World Journal, 2014
Newcastle disease virus (NDV) causes large losses in the global fowl industry. To better understand NDV replication and transcription cycle, quantitative detection methods for distinguishing NDV genomic RNA (gRNA), antigenomic RNA (cRNA), and messenger ...
Xusheng Qiu, Yang Yu, Shengqing Yu, Yuan Zhan, Nana Wei, Cuiping Song, Yingjie Sun, Lei Tan, Chan Ding   +8 more
doaj   +1 more source