Results 111 to 120 of about 46,126 (304)

Table1_gRNA-SeqRET: a universal tool for targeted and genome-scale gRNA design and sequence extraction for prokaryotes and eukaryotes.XLSX

, 2023
High-throughput genetic screening is frequently employed to rapidly associate gene with phenotype and establish sequence-function relationships. With the advent of CRISPR technology, and the ability to functionally interrogate previously genetically ...
Jan-Fang Cheng (48619), Lisa Simirenko (74525), Ian Blaby (8204187)   +2 more
core   +1 more source

Targeting the YAP‐BST2 Axis Overcomes Intrinsic Anti‐PD‐1 Resistance in Metastatic Gastric Cancer

Advanced Science, EarlyView.
Concurrent YAP hyperactivation and Tp53 loss in Atp4b‐expressed parietal cell lineage drives gastric cancer initiation, multi‐organ metastasis, and intrinsic resistance to anti‐PD‐1 therapy. BST2 emerges as a direct YAP‐TEAD transcriptional target that engages PIRA2 on myeloid cells to establish an immunosuppressive microenvironment.
Weihong Zhang, Shilong Wang, Meng Wang, Ruixian Yu, Jingwu Yue, Lin Shao, Hui Zhang, Mengwen Zhu, Luyang Tian, Shuting Cheng, Weimin Qin, Yang Tang, Yi Han, Wenjia Wang, Liwei An, Yan Meng, Shi Jiao, Zhaocai Zhou   +17 more
wiley   +1 more source

Role of Purine-Rich Regions in Mason-Pfizer Monkey Virus (MPMV) Genomic RNA Packaging and Propagation

Frontiers in Microbiology, 2020
A distinguishing feature of the Mason-Pfizer monkey virus (MPMV) packaging signal RNA secondary structure is a single-stranded purine-rich sequence (ssPurines) in close vicinity to a palindromic stem loop (Pal SL) that functions as MPMV dimerization ...
Lizna Mohamed Ali, Fathima Nuzra Nagoor Pitchai, Valérie Vivet-Boudou, Akhil Chameettachal, Ayesha Jabeen, Vineeta N. Pillai, Farah Mustafa, Farah Mustafa, Roland Marquet, Tahir A. Rizvi, Tahir A. Rizvi   +10 more
doaj   +1 more source

Hazelnut allergome overview and Cor a gRNAs identification

BMC Plant Biology
Corylus species (hazelnuts) are a valuable source of nutrients and are widely consumed worldwide. Nevertheless, Corylus avellana (Cor a) contains 13 allergens (Cor a 1, Cor a 2, Cor a 6, Cor a 8, Cor a 9, Cor a 10, Cor a 11, Cor a 12, Cor a 13, Cor a 14, Cor a 15, Cor a 16, and Cor a TLP) that have been deposited into the official database (WHO/IUIS ...
Amoroso, Ciro Gianmaria, Andolfo, Giuseppe   +1 more
openaire   +4 more sources

Exploring Non-transgenic CRISPR-Cas9 gRNA Delivery Using Transactivation in Nicotiana benthamiana [PDF]

, 2020
Gene editing via CRISPR-Cas9 (Clustered, Regularly Interspaced, Short Palindromic Repeats – CRISPR-associated protein 9) is a powerful tool in biotechnology.

core   +2 more sources

Design of a Novel Diamine Biosensor to Guide the Engineering of Escherichia coli for Cadaverine Overproduction

Advanced Science, EarlyView.
A high‐throughput screening platform was established by integrating an in vivo cadaverine biosensor with a fluorescence‐activated cell sorting (FACS) system. Biosensor‐driven specific exporter tuning, pathway optimization and genome‐wide target mining were conducted to maximize cadaverine production in Escherichia coli.
Jia Feng, Getao Liu, Ding Ma, Jiaxiang Yuan, Xinyi Zhang, Weihao Wang, Chen Ma, Yang Liao, Siyuan Gao, Lei Qin, Jiao Feng, Sheng Xu, Xin Wang, Kequan Chen   +13 more
wiley   +1 more source

TP53 Loss Elevates NF‐κB‐IFN‐β‐MHC‐Ia Signaling to Promote NK Cell Resistance in Osteosarcoma

Advanced Science, EarlyView.
TP53 loss in a transforming or osteosarcoma cell promotes cytosolic DNA accumulation, activating NF‐κB‐dependent IFN‐β production. Autocrine IFN‐β signaling increases cell‐surface HLA‐Ia expression, strengthens inhibitory KIR signaling in natural killer cells, and thereby enables the affected cell to evade NK cell‐mediated cytotoxicity.
Guihui Qin, Cheung Kwan Yeung, Ye Yi, Dejin Zheng, Miaoman Ye, Chi‐Chong Chio, Jinjie Wu, Siyi Fu, Chu‐Xia Deng, Ren‐He Xu   +9 more
wiley   +1 more source

CRISPR/Cas9-Mediated Three Nucleotide Insertion Corrects a Deletion Mutation in MRP1/ABCC1 and Restores Its Proper Folding and Function

Molecular Therapy: Nucleic Acids, 2017
A three-nucleotide deletion in cystic fibrosis transmembrane conductance regulator/ATP-binding cassette transporter C7 (CFTR/ABCC7) resulting in the absence of phenylalanine at 508 leads to mis-fold of the mutated protein and causes cystic fibrosis.
Qinqin Xu, Yue-xian Hou, Xiu-bao Chang
doaj   +1 more source

Are the current gRNA ranking prediction algorithms useful for genome editing in plants?

PLoS ONE, 2020
Introducing a new trait into a crop through conventional breeding commonly takes decades, but recently developed genome sequence modification technology has the potential to accelerate this process. One of these new breeding technologies relies on an RNA-
Fatima Naim, Kylie Shand, Satomi Hayashi, Martin O'Brien, James McGree, Alexander A T Johnson, Benjamin Dugdale, Peter M Waterhouse   +7 more
doaj   +1 more source

Multiplexed gene activation using the SAM system with gRNA arrays.

, 2018
(a) Diagram depicting the elements encoded in plasmids used for multiplex gene activation using the SAM system combined with gRNA arrays containing MS2 sequences. (b) RT-PCR results of gene activation at 5 gRNA target sites 3 days post transfection using
Kai Hui (5753966), Natalie K. Wolf (5753951), Walker S. Lahr (5753954), Mitchell G. Kluesner (5753960), Samantha Lee (5753963), Morito Kurata (154677), Madison T. Weg (5753957), Masano Shiraiwa (5753969), Branden S. Moriarity (559376), Beau R. Webber (5753972)   +9 more
core   +1 more source