Results 131 to 140 of about 46,126 (304)

Epithelial SLC25A20 Deficiency Drives Immunosuppressive Neutrophil Remodeling via ARG1 Palmitoylation to Promote Colorectal Cancer

Advanced Science, EarlyView.
SLC25A20 deficiency in tumor cells compromises mitochondrial LCFA transport, which suppresses FAO and drives compensatory release of palmitic acid‐enriched lipids into the TME. These tumor‐derived palmitic acids are taken up by TANs, promoting ZDHHC18‐catalyzed ARG1 palmitoylation.
Yue Han, Tian‐Yuan Tan, Bin Zhang, Ye‐Bin Fu, Wen Chen, Min‐Hui Yang, Yi‐Qing Wang, Yan‐Qing Ding, Shuang Wang   +8 more
wiley   +1 more source

Fragment analysis testing of 5 different gRNA designs.

, 2019
a. Digital gel image of products resulting from Cas9 digestion of CTG18.1 plasmid with five different gRNAs (lanes 1–5). No gRNA was added to the sample shown in lane C. b.
Shubham Basu (6920210), Malinda L. Butz (297437), Eric D. Wieben (278400), Cristiane M. Ida (494909), Keith H. Baratz (297439), Jenny M. Ekholm (6920219), Vivekananda Sarangi (497993), Brett Bowman (745776), Michael P. Fautsch (292351), John R. Mills (2815033), Edward W. Highsmith (6920216), Ross A. Aleff (297436), Ian J. McLaughlin (6920213)   +12 more
core   +1 more source

Scaffold RNA Reengineering for a Leakage‐Suppressed CRISPR/Cas12a Cascade System Toward Precise Nucleic Acid Diagnostics

Advanced Science, EarlyView.
Schematic illustration of the CALCas system, in which a conformational switch in the 3' terminal DNA extension of scaffold RNA (3'D‐SR) drives cascade‐activatable Cas12a cleavage while suppressing leakage. ABSTRACT Amplification‐free CRISPR detection has attracted significant attention for its capacity to circumvent the intricacies of nucleic acid ...
Xingrong Li, Honghua Hu, Xinli Jiang, Xuhuai Fu, Lin Hu, Qinghua Wu, Jiatong Chai, Diangeng Li, Jinhong Guo, Zhiqiang Li, Yi Li, Yirong Li   +11 more
wiley   +1 more source

Large Oncosomes Induce Pro‐Metastatic Stromal‐Myeloid Reprogramming

Advanced Science, EarlyView.
Large oncosomes systemically reprogram bone marrow mesenchymal stem cells through innate nucleic acid sensing, driving inflammatory and immunosuppressive remodeling that supports a metastasis‐permissive bone marrow environment, revealing a novel tumor‐host communication axis in cancer. ABSTRACT Bone metastasis can be facilitated by systemic remodeling,
Taylon Felipe Silva, Virginia Marcia Concato‐Lopes, Fatima Bedier, Lauren Newman, Diana Kitka, Jamelle Brown, Blandine Victor, Minhyung Kim, Tatyana Vagner, Catherine Grasso, Dmitriy Sheyn, Sungyong You, Michael R. Freeman, Fayyaz S. Sutterwala, Helen S. Goodridge, Caroline Jefferies, Paola de Candia, Jlenia Guarnerio, Dolores Di Vizio   +18 more
wiley   +1 more source

In Vivo CRISPR Screen Identifies RPS19 as an Immunotherapy Target in Hepatocellular Carcinoma

Advanced Science, EarlyView.
By employing in vivo CRISPR functional screening, we identify ribosomal protein RPS19 as a pivotal mediator driving immune evasion in hepatocellular carcinoma, wherein tumor‑released RPS19 binds C5aR1 on tumor‑associated macrophages to initiate downstream immunosuppressive signaling; SP94‑peptide‑modified SNPSS nanoparticles deliver Cas9/sgRPS19 ...
Lei He, Duo Yu, Fei Xie, Kaisheng Hou, Zhaozhao Li, Meifang Dai, Jingyi Yun, Haiyan Cao, Yang Xiao, Yueyuan Qiu, Gege Shi, Wangqian Zhang, Shuning Wang, Qiang Hao, Jieyu Zhang, Zhaowei Wang, Yingqi Zhang, Kuo Zhang, Jintao Gu, Haichen Du, Meng Li   +20 more
wiley   +1 more source

Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER

Cell Genomics
Summary: Single-cell RNA sequencing CRISPR (perturb-seq) screens enable high-throughput investigation of the genome, allowing for characterization of thousands of genomic perturbations on gene expression. Ambient gRNAs, which are contaminating gRNAs, are
Siyan Liu, Marisa C. Hamilton, Thomas Cowart, Alejandro Barrera, Lexi R. Bounds, Alexander C. Nelson, Sophie F. Dornbaum, Julia W. Riley, Richard W. Doty, Andrew S. Allen, Gregory E. Crawford, William H. Majoros, Charles A. Gersbach   +12 more
doaj   +1 more source

mRNA/gRNA binding affinity by EMSA.

, 2013
A–D: Representative images of 6% polyacrylamide gels and corresponding binding isotherms with the apparent dissociation constants (KD). Samples contained 5 nM 32P-labeled gRNA (gA6-14, gCYb-558 and gND7-550) or 10 nM 32P-labeled gRNA (gND7-506) and ...
Donna J. Koslowsky (362427), Larissa Reifur (362422), Jorge Cruz-Reyes (12817), Laura E. Yu (362424), Michelle vanHartesvelt (362426)   +4 more
core   +1 more source

Lactate Drives Pathological Browning of White Adipose Tissue to Aggravate Sepsis‐Associated Metabolic Disorders in a GPR81‐Dependent Manner

Advanced Science, EarlyView.
Sepsis‐triggered lactate accumulation induces pathological white adipose tissue browning. Lactate activates adipocyte GPR81 to sequentially upregulate CTRP6‐p38 signalling and UCP1 expression. This cascade exacerbates sepsis‐associated metabolic disorders.
Chunliang Zheng, Xiaohua Li, Shuo Li, Chungen Xing, Chun Cao   +4 more
wiley   +1 more source

BEstimate: a computational tool for the design and interpretation of CRISPR base editing experiments

Genome Biology
CRISPR base editors enable scalable targeted DNA mutagenesis and are a powerful tool for analysing the function of variants of uncertain significance and disease modelling.
Cansu Dinçer, Bo Fussing, Mathew J. Garnett, Matthew A. Coelho   +3 more
doaj   +1 more source

Cas9/gRNA-mediated multiple gene disruptions in goat fibroblasts.

, 2014
Cas9/gRNA-mediated multiple gene disruptions in goat fibroblasts.
Xinxia Zhao (625584), Jun Qiao (328589), Shengwei Hu (392403), Chuangfu Chen (328590), Misha Regouski (625585), Min Yang (146229), Wei Ni (286780), Irina A. Polejaeva (504731)   +7 more
core   +1 more source