Results 131 to 140 of about 44,236 (298)
Cell GenomicsSummary: Single-cell RNA sequencing CRISPR (perturb-seq) screens enable high-throughput investigation of the genome, allowing for characterization of thousands of genomic perturbations on gene expression. Ambient gRNAs, which are contaminating gRNAs, are
Siyan Liu +12 more
doaj +1 more source
Advanced Science, EarlyView.Sun et al. report a self‐assembling trimeric subunit vaccine platform termed RHS, which integrates the JN.1 receptor‐binding domain (RBD) with the heptad repeat 1 (HR1), stem helix (SH), and heptad repeat 2 (HR2) regions. The SARS‐CoV‐2 RHS vaccine elicits variant‐reactive RBD‐specific antibodies and cross‐reactive SH‐specific antibodies against ...
Xikui Sun +18 more
wiley +1 more source
Dynamic Operations in Macromolecular Data Storage
Angewandte Chemie, EarlyView.With the exponential growth of digital data and the limitations of conventional silicon‐based storage and computing technologies, macromolecular data storage, the practice of encoding digital information within large, complex molecules like DNA or synthetic polymers, has emerged as a scalable and sustainable alternative to traditional digital ...
Jakub Ossowski +3 more
wiley +2 more sources
Survey of 3 prime GG gRNA sites in 8 genomes
, 2015 gRNA sites with a 3' GG motif adjacent to the PAM site have been shown to exhibit substantially higher genome-editing efficiency in C. elegans. I scanned 6 model genomes (Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Danio ...
Elisha Roberson (98532)
core +1 more source
Advanced Science, EarlyView.An optimized Cas9‐enriched nanopore sequencing workflow, combined with STRiker, enables simultaneous analysis of disease‐associated STR loci from patient blood. The nCATS–STRiker workflow detects repeat expansions, de novo repeat motifs, interruption patterns, and methylation in a single assay, improving the genetic diagnosis of previously undiagnosed ...
Seungbok Lee +11 more
wiley +1 more source
Advanced Science, EarlyView.D4F‐functionalized ceria nanozyme–CasRx nanoparticles preferentially target renal macrophages through SR‐B1‐mediated uptake in ischemia–reperfusion injury. Ceria scavenges excessive ROS, while CasRx silences STING signaling, shifting macrophages toward a less inflammatory, pro‐resolving state and increasing the proportion of regulatory T cells.
Weibo Chen +11 more
wiley +1 more source
Fragment analysis testing of 5 different gRNA designs.
, 2019 a. Digital gel image of products resulting from Cas9 digestion of CTG18.1 plasmid with five different gRNAs (lanes 1–5). No gRNA was added to the sample shown in lane C. b.
Shubham Basu (6920210) +12 more
core +1 more source
Advanced Science, EarlyView.CDegSR, a new tool for imaging RNA at single molecule level in living cells was developed by researchers. Background noise was eliminated by protein engineering and making it degrade when not bound to its target. This allowed individual RNA molecules to be clearly imaged and tracked.
Shipeng Shao, Hongchen Zhang
wiley +1 more source
Advanced Science, EarlyView.This work develops a metabolic sharing strategy that employs cytidine monophosphate (CMP) to competitively reprogram floxuridine (FUDR) pyrimidine metabolism in colorectal tumor cells. Elevated intracellular FdUMP induces nucleotide imbalance, drives mitochondrial DNA release and cGAS‐STING innate immune cascade activation, and potentiates robust ...
Chao Wang +10 more
wiley +1 more source
3-color system for studying HIV-1 gRNA trafficking.
, 2016 (A) Depiction of surrogate gRNA transcripts bearing either the RRE (RRE-gRNA) or lacking an export element (ΔEE-gRNA). Transcripts retained the native viral leader sequence including the packaging signal and include the major splice donor (SD) and two ...
Nathan M. Sherer (86591) +4 more
core +1 more source