Results 151 to 160 of about 44,236 (298)

Bridging the translational gap in clinical nanomedicine: From rational design to clinical reality

BMEMat, EarlyView.
Nanotechnology offers a multi‐faceted approach to modern healthcare, encompassing high‐sensitivity diagnostic imaging, innovative vaccine delivery, and targeted therapeutics. This review explores how these nanomedicine applications address critical challenges in cancer recurrence and the treatment of neurologic and respiratory diseases to combat rising
Ahmed H. Ghonaim, Marwa Ahmed El‐Naka, Wedad M. Nageeb, Atta Ullah Khan, Noura M. Seleem, Aliaa Abdelghafar, Doaa Zamel, Yang Liu, Qigai He, Wentao Li   +9 more
wiley   +1 more source

Cas9/gRNA-mediated multiple gene disruptions in goat fibroblasts.

, 2014
Cas9/gRNA-mediated multiple gene disruptions in goat fibroblasts.
Xinxia Zhao (625584), Jun Qiao (328589), Shengwei Hu (392403), Chuangfu Chen (328590), Misha Regouski (625585), Min Yang (146229), Wei Ni (286780), Irina A. Polejaeva (504731)   +7 more
core   +1 more source

RNA‐centric world of retroviruses: unravelling the molecular strategies of genomic RNA packaging

Biological Reviews, EarlyView.
ABSTRACT Retroviruses constitute a unique group of RNA viruses that have profoundly influenced both evolutionary trajectories and biomedical research. Their ability to reverse transcribe and integrate into host genomes has shaped genomic architecture across species and contributed to our understanding of oncogenes, gene regulation, and RNA biology ...
Mohammad Abdullah Jehad, Suresha G. Prabhu, Lizna M. Ali, Vineeta N. Pillai, Farah Mustafa, Tahir A. Rizvi   +5 more
wiley   +1 more source

Map of the H11 locus in the CHO-S genome and gRNA validation.

, 2019
(A) The genomic region between the EIF4ENIF1 and DRG1 genes was targeted for CRISPR knock-in. Targeting locations of the two gRNA candidates, g1 and g2, are shown.
Ling-Jie Kong (7042007), Xiuling Chi (2023792), Ruhong Jiang (7042001), Ruby Yanru Chen-Tsai (7042004), Qi Zheng (241020)   +4 more
core   +1 more source

4′‐ThioRNA Substitution and Sense‐Strand Segmentation for the Design of Functional Fully Modified siRNAs Targeting SARS‐CoV‐2

Chemistry – A European Journal, EarlyView.
4′‐ThioRNA was broadly tolerated across multiple positions in SARS‐CoV‐2‐targeting siRNAs, but full 4'‐thio modification of both strands caused a reduction in RNAi activity. Sense‐strand segmentation improved RNAi and antiviral activity, particularly when the nick was located at position 10 from the 5′‐end of the sense strand, thereby unlocking the ...
Yuhei Nogi, Jun Tsukimoto, Noriko Saito‐Tarashima, Takaaki Koma, Masako Nomaguchi, Noriaki Minakawa   +5 more
wiley   +1 more source

MIT/TFE family transcription factors, Mitf and Tfec, redundantly promote pigment cell differentiation and retinal epithelium pigmentation in teleosts

Developmental Dynamics, EarlyView.
Abstract Background Transcription factors of the MIT/TFE family—mitfa, mitfb, and tfec—play essential roles in specifying pigment cells derived from neural crest cells in vertebrates. In teleosts, which possess multiple pigment cell types, these factors exhibit partially redundant but largely distinct functions across different pigment lineages.
James Lister, Keisuke Sugiwaka, Tetsuaki Kimura, Rieko Tanaka, Makoto Goda, Hikaru Kobayashi, Chikako Inoue, Kawilarang W. A. Masengi, Kazunori Yamahira, Kiyoshi Naruse, Satoshi Ansai, Robert N. Kelsh, Masahiko Hibi, Hisashi Hashimoto   +13 more
wiley   +1 more source

Determination of gRNA Efficiency In Vivo v1

Dissecting how specific genes causally contribute to the function of defined cell types requires knocking them out in vivo. While CRISPR/Cas9 has revolutionized gene editing and genomic engineering, methods to characterize its editing efficiency after in vivo expression have been lacking. This protocol addresses that gap by quantifying guide RNA (gRNA)
Carl V. L. Olson, Ryan Gillis, Molly Logsdon, Bowen Tan   +3 more
openaire   +1 more source

Assembly of mitochondrial ribonucleoprotein complexes involves specific guide RNA (gRNA)-binding proteins and gRNA domains but does not require preedited mRNA.

, 1994
RNA editing in kinetoplastids probably employs a macromolecular complex, the editosome, that is likely to include the guide RNAs (gRNAs) which specify the edited sequence. Specific ribonucleoprotein (RNP) complexes which form in vitro with gRNAs (H.
Stuart, K., Read, L. K., Göringer, H. Ulrich   +2 more
core  

Summary of the gRNA data coverage for each gene.

, 2016
Summary of the gRNA data coverage for each gene.
Laura E. Kirby (3155058), David Judah (166627), Donna Koslowsky (3155064), Scooter Nowak (3155061), Yanni Sun (27928)   +4 more
core   +1 more source

Computational Intelligence Accelerates CRISPR‐Cas‐Based Diagnostic Design

iLABMED, EarlyView.
Computational intelligence, including pangenome‐guided target mining, machine‐learning‐based guide RNA optimization, and autonomous AI agents, is shifting CRISPR diagnostic design from empirical screening to systematic, data‐driven engineering and accelerating the path from pathogen discovery to deployable diagnostics.
Hengyu Wang, Qiurun He, Xuhu Mao, Yong Sheng   +3 more
wiley   +1 more source