Results 141 to 150 of about 46,126 (304)
Advanced Science, EarlyView.MSNs serve as a robust vehicle for CRISPRa‐mediated Reln induction, driving bone repair through the establishment of a neurogenic‐supportive microenvironment. ABSTRACT Neurogenic bone regeneration is essential for the effective restoration of bone tissue functionality, while the mechanisms and new strategies underlying neurogenic bone regeneration ...
Shuai Lin +9 more
wiley +1 more source
3-color system for studying HIV-1 gRNA trafficking.
, 2016 (A) Depiction of surrogate gRNA transcripts bearing either the RRE (RRE-gRNA) or lacking an export element (ΔEE-gRNA). Transcripts retained the native viral leader sequence including the packaging signal and include the major splice donor (SD) and two ...
Nathan M. Sherer (86591) +4 more
core +1 more source
Combinatorial Tuning of Multigene Biosynthetic Pathways based on RNA Interference
Advanced Science, EarlyView.Small RNA sequencing identifies candidate endogenous Pol III promoters, and subsequent fluorescence screening measures knockdown efficiency to assess promoter strength. Combining promoter strength and shRNA target position enables fine‐grained RNAi regulation of multigene pathways. This strategy enhances β‐carotene production in Schizochytrium sp.
Zixu Zhang +6 more
wiley +1 more source
Summary of the gRNA data coverage for each gene.
, 2016 Summary of the gRNA data coverage for each gene.
Laura E. Kirby (3155058) +4 more
core +1 more source
TIPIN Couples Epigenetic Rewiring to EGFR Stabilization and Lenvatinib Resistance in Liver Cancer
Advanced Science, EarlyView.DNA hypomethylation elevates TIPIN expression in liver cancer. TIPIN competes with E3 ligase c‐Cbl for the EGFR intracellular domain, inhibiting EGFR ubiquitination and lysosomal degradation to sustain oncogenic EGFR‐ ERK signaling. High TIPIN confers lenvatinib resistance, while TIPIN depletion restores drug sensitivity in liver cancer. ABSTRACT Aims:
Xing Jia +15 more
wiley +1 more source
MOESM11 of gRNA validation for wheat genome editing with the CRISPR-Cas9 system
, 2019 Additional file 11 Homoeoallele-specific amplification of EPSPS on chromosomes 7AS, 4AL and 7DS following transient co-expression of Cas9 and gRNA in wheat protoplasts. Nulli-tetra (â) genomic DNA template does not contain the target chromosome.
Ryan Whitford (6055841) +5 more
core +1 more source
Advanced Science, EarlyView.Pharmacokinetic tuning acts as a programmable mechanistic switch for β‐lap. Sustained low‐concentration exposure redirects β‐lap from NQO1 bioactivation to direct oxidation of vimentin, driving disulfide crosslinking, proteasomal overload and ferroptosis.
Lei Dou +5 more
wiley +1 more source
gRNA generated using ribozymes is as efficient as gRNA generated by transcription alone.
, 2016 PAGE analysis of smo 5’ UTR shows the formation of heteroduplex after injection of gRNA and Cas9 mRNA compared to the uninjected control. Comparison of intensity of heteroduplex and homoduplex shows that gRNA with or without ribozymes have similar ...
Ashley Shu Mei Ng (493205) +2 more
core +1 more source
Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing
Animal Research and One Health, EarlyView.Multiplex genome editing is transforming genome engineering from single‐gene perturbation to network‐level control, yet its broader application remains limited by challenges in gRNA array engineering, delivery technologies, and safety management. Emerging AI‐driven approaches are accelerating guide RNA design and CRISPR effector optimization for ...
Linli Wang, Yongbin Liu, Hongbing Han
wiley +1 more source
CRISPR-Cas guide RNA indel analysis using CRISPResso2 with Nanopore sequencing data
BMC Research NotesObjective Insertion and deletion (indel) analysis of CRISPR-Cas guide RNAs (gRNAs) is crucial in gene editing to assess gRNA efficiency and indel frequency.
Gus Rowan McFarlane +2 more
doaj +1 more source